Correlation between cell survival and micronuclei formation in V79 cells treated with vindesine before exposure to different doses of γ-radiation

Jagetia, Ganesh Chandra; Adiga, Satish Kumar

doi:10.1016/s0027-5107(99)00240-7

Cited by 17 publications

(9 citation statements)

References 38 publications

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“…The relationship between lethal events (micronucleus) and surviving fraction was found to be linear quadratic. A similar relationship was reported earlier (Jagetia and Adiga, 1995;Jagetia and Adiga, 1997;Jagetia and Adiga, 2000). A close correlation between cell survival and micronuclei-induction has been observed (Midander and Revesz, 1980;Stap and Aten, 1990;Abend et al, 1995).…”

Section: Discussionsupporting

confidence: 88%

“…Several chemotherapeutic agents such as vincristine, vinblastine, vindesine, taxol, doxorubicin and teniposide have been reported to induce micronuclei (Jagetia and Jacob, 1992;Jagetia and Nayak, 1996;Adiga, 1995, 1998;Adiga and Jagetia, 1999;Jagetia and Baliga, 2002). Similarly, ionizing radiations have been reported to increase the frequency of micronuclei in a dose dependent manner in vitro and in vivo (Jagetia and Ganapathi, 1994;Jagetia and Adiga, 2000;Jagetia and Reddy, 2002). This increase in micronucleus formation after bleomycin treatment was two to three fold higher at a concentration of 10 µg ml −1 and more.…”

Section: Discussionmentioning

confidence: 96%

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Naringin, a grapefruit flavanone, protects V79 cells against the bleomycin‐induced genotoxicity and decline in survival

Jagetia

Jha

2006

J of Applied Toxicology

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The effect of naringin, a grapefruit flavonone was studied on bleomycin-induced genomic damage and alteration in the survival of cultured V79 cells. Exposure of V79 cells to bleomycin induced a concentration dependent elevation in the frequency of binucleate cells bearing micronuclei (MNBNC) and a maximum number of MNBNCs were observed in the cells treated with 50 microg ml(-1) bleomycin, the highest concentration evaluated. This genotoxic effect of bleomycin was reflected in the cell survival, where a concentration dependent decline was observed in the cells treated with different concentrations of bleomycin. Treatment of cells with 1 mm naringin before exposure to different concentrations of bleomycin arrested the bleomycin-induced decline in the cell survival accompanied by a significant reduction in the frequency of micronuclei when compared with bleomycin treatment alone. The cell survival and micronuclei induction were found to be inversely correlated. The repair kinetics of DNA damage induced by bleomycin was evaluated by exposing the cells to 10 microg ml(-1) bleomycin using single cell gel electrophoresis. Treatment of V79 cells with bleomycin resulted in a continuous increase in DNA damage up to 6 h post-bleomycin treatment as evident by migration of more DNA into the tails (% tail DNA) of the comets and a subsequent increase in olive tail moment (OTM), an index of DNA damage. Treatment of V79 cells with 1 mm naringin reduced bleomycin-induced DNA damage and accelerated DNA repair as indicated by a reduction in % tail DNA and OTM with increasing assessment time. A maximum reduction in the DNA damage was observed at 6 h post-bleomycin treatment, where it was 5 times lower than bleomycin alone. Our study, which was conducted on the basis of antioxidant, free radical scavenging and metal chelating properties of naringin demonstrates that naringin reduced the genotoxic effects of bleomycin and consequently increased the cell survival and therefore may act as a chemoprotective agent in clinical situations.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 96%

Naringin, a grapefruit flavanone, protects V79 cells against the bleomycin‐induced genotoxicity and decline in survival

Jagetia

Jha

2006

J of Applied Toxicology

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“…Treatment of HeLa cells with different concentrations of TCE before irradiation caused radiation dose-dependent decline in the cell viability. An identical effect has been observed earlier where, various extracts of giloe were found to increase the effect of γ-radiation [44]. Similarly, berberine, an alkaloid present in the giloe has been found to increase the effect of ionizing radiation in HeLa cells by Pratt and Willis test [45].…”

Section: Discussionsupporting

confidence: 76%

Dichloromethane Extract of Giloe (Tinospora Cordifolia, Wild) Increases the Radiosensitivity of Cultured Hela Cells Exposed to Different Doses of Γ-Radiation

Gc¹

2017

JONAM

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Ionizing radiations are in frequent clinical use to treat cancer either alone or in combination with surgery or chemotherapy. The radioresistance of tumors is a stumbling block to realize the full potential of radiotherapy. Therefore, pharmacophores that reduce the radioresistance of tumors may be of great importance during tumor therapy. In the present study an attempt has been made to evaluate the potential of dichloromethane extract of giloe i.e. Tinospora cordifolia (TCE) in cultured HeLa cells. Exposure of HeLa cells to TCE for 4 h before exposure to 2 Gy γ-radiation led to a significant decrease in the cell viability (approximately 50 %) accompanied by a reduction in the surviving fraction (SF) up to 0.52 after 4 h of TCE treatment. Thereafter, clonogenecity of HeLa cells declined negligibly with increase in treatment duration up to 6 h post-treatment. There has been a dose dependent attrition in the cell viability of HeLa cells exposed to 1-4 Gy γ-irradiation, whereas treatment of HeLa cells with various doses of TCE further decreased the cell viability depending not only on the irradiation dose but also on the concentration of TCE. The irradiation of HeLa cells resulted in a radiation dose dependent decline in the SF and increasing TCE concentration before irradiation caused a further TCE concentration dependent reduction in SF, and a lowest SF was observed for 4 µg/ml TCE for all irradiation doses. Treatment of HeLa cells with different concentrations of TCE before 3 Gy irradiation resulted in a concentration dependent depletion in glutathione-S-transferase activity until 12 h post-irradiation, whereas lactate dehydrogenase and lipid peroxidation increased up to 4 h post-irradiation and declined gradually thereafter up to 12 h post-irradiation. The TCE treatment increased the radiosensitivity of HeLa cells by reducing glutathione-S-transferase activity and increasing the activity of lactate dehydrogenase and lipid peroxidation.

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“…A similar effect has been observed for V79 cells treated with taxol, vindesine, and teniposide before irradiation. [17][18][19] Lipid peroxidation and LDH are hallmarks of membrane damage. Lipid peroxidation is an important event related to cell death and has been reported to cause severe impairment of membrane function through increased membrane permeability and membrane protein oxidation that eventually leads to cell death by damaging the cellular DNA.…”

Section: Discussionmentioning

confidence: 99%

Alteration in the Radiosensitivity of HeLa Cells by Dichloromethane Extract of Guduchi (Tinospora cordifolia)

Rao

2010

Integr Cancer Ther

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Exposure of HeLa cells to TCE (dichloromethane extract of Tinospora cordifolia) for 4 hours before exposure to 2-Gy γ-radiation caused a significant decrease in the cell viability (approximately 50%). The surviving fraction (SF) was reduced to 0.52 after 4 hours of TCE treatment; thereafter, clonogenecity of HeLa cells declined negligibly with treatment duration up to 6 hours posttreatment. Exposure of HeLa cells to different doses of γ-radiation resulted in a dose-dependent decline in the viability of HeLa cells, whereas treatment of HeLa cells with various doses of TCE further decreased the cell viability depending not only on the irradiation dose but also on the concentration of TCE. Treatment of HeLa cells with various doses of TCE caused a significant decline in cell viability after exposure to 1 to 4 Gy γ-radiation. The increase in TCE concentration before irradiation caused a concentration-dependent reduction in the SF, and a lowest SF was observed for 4 μg/mL TCE for all exposure doses. HeLa cells treated with TCE showed an increase in lactate dehydrogenase and decrease in glutathione-S-transferase activity at all postirradiation times. Lipid peroxidation increased up to 4 hours postirradiation and declined gradually up to 12 hours postirradiation.

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Correlation between cell survival and micronuclei formation in V79 cells treated with vindesine before exposure to different doses of γ-radiation

Cited by 17 publications

References 38 publications

Naringin, a grapefruit flavanone, protects V79 cells against the bleomycin‐induced genotoxicity and decline in survival

Naringin, a grapefruit flavanone, protects V79 cells against the bleomycin‐induced genotoxicity and decline in survival

Dichloromethane Extract of Giloe (Tinospora Cordifolia, Wild) Increases the Radiosensitivity of Cultured Hela Cells Exposed to Different Doses of Γ-Radiation

Alteration in the Radiosensitivity of HeLa Cells by Dichloromethane Extract of Guduchi (Tinospora cordifolia)

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