Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

Liu, Qian; Xu, Bo; Zhou, Wan-Shan

doi:10.3892/ol.2017.7355

Cited by 9 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multiple markers of tumor aggressiveness have been identified in exploratory studies, and while not prospectively validated may provide insight into the biology of these tumors. In osteosarcoma mouse models, ezrin was associated with increased metastatic potential and poorer prognosis while high ezrin expression correlated with chemotherapy resistance (6,17). Over-expression of MTA-1 has been noted in high-grade osteosarcoma tumor samples with little to no expression in low-grade samples (9).…”

Section: Discussionmentioning

confidence: 99%

Differential Outcomes and Biologic Markers of Radiation-Associated vs. Sporadic Osteosarcoma: A Single-Institution Experience

et al. 2020

View full text Add to dashboard Cite

Background: Radiation-associated osteosarcoma (RAO) is a rare, life-threatening complication from radiation. Many physicians presume RAO has a worse prognosis than sporadic osteosarcoma (SO), although limited objective data exist. We conducted a retrospective study comparing these entities. Methods: We identified adults treated at our institution with osteosarcoma (1990-2016) and categorized tumors as SO or RAO based on location within a prior radiation field. We extracted data on demographics, treatment and primary malignancy and examined available tumor samples for MTA-1 and ezrin using immunohistochemistry (IHC). Results: Of 159 identified patients, 28 had RAO, diagnosed at a median interval from radiation of 11.5 years (1.5-28 years). Median follow-up was 2.8 years (0.1-19.6 years). Median progression free survival (PFS) and overall survival (OS) were not significantly different in the small population of patients with metastases, SO (n = 20) vs. RAO (n = 6): PFS 10.3 months vs. 4.8 months (p = 0.45) and OS 15.6 months vs. 6.1 months (p = 0.96), respectively. For the larger group with localized disease, median relapse-free survival (RFS) and OS were significantly different, NR vs. 12.2 months (p < 0.001) and NR vs. 27.6 months (p = 0.001) in SO (n = 111) vs. RAO (n = 22), respectively. On IHC, there were significant differences in distribution of high, intermediate or low MTA-1 (p = 0.015) and ezrin (p = 0.002) between RAO and SO tumors. Conclusions: Patients with metastases at diagnosis fared poorly irrespective of prior radiation. RAO patients with localized disease had worse outcomes without detectable differences in therapy rendered or treatment effect in resected specimens. Higher expression of MTA-1 in RAO patients may suggest an underlying difference in tumor biology to explain differences in outcomes.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential Outcomes and Biologic Markers of Radiation-Associated vs. Sporadic Osteosarcoma: A Single-Institution Experience

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Furthermore, ezrin deletion in autophagic TAMs decreased TGF-β1 expression and contributed to EMT as well as the cell migration and invasion ability of lung adenocarcinomas. Ezrin, an important regulatory protein that connects cell membrane molecules, is highly expressed in various solid tumors and is associated with poor prognosis (35,36). Chen et al reported that ezrin can mediate EMT in A549 cells through the TGF-β1 pathway (37).…”

Section: Discussionmentioning

confidence: 99%

Autophagic tumor-associated macrophages promote the endothelial mesenchymal transition in lung adenocarcinomas through the FUT4/p-ezrin pathway

Wang¹,

Chen²

2021

J Thorac Dis

View full text Add to dashboard Cite

“…Statistical significance was considered as p ≤ 0.05. Further, for correlations between Mthfr and genes involved in hepatic lipid metabolism, Pearson’s correlation coefficient was computed [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Sclerocarya birrea (Marula) Extract Inhibits Hepatic Steatosis in db/db Mice

Mabasa

Kotze

Shabalala

et al. 2022

IJERPH

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) is a spectrum of hepatic metabolic perturbations ranging from simple steatosis to steatohepatitis, cirrhosis and hepatocellular carcinoma. Currently, lifestyle modifications to reduce weight gain are considered the most effective means of preventing and treating the disease. The aim of the present study was to determine the therapeutic benefit of Sclerocarya birrea (Marula leaf extract, MLE) on hepatic steatosis. Obese db/db mice were randomly stratified into the obese control, metformin (MET) or MLE-treated groups. Mice were treated daily for 29 days, at which point all mice were euthanized and liver samples were collected. Hematoxylin and eosin staining was used for histological assessment of the liver sections, while qRT-PCR and Western blot were used to determine hepatic mRNA and protein expression, respectively. Thereafter, the association between methylenetetrahydrofolate reductase (Mthfr a key enzyme in one-carbon metabolism and DNA-methylation-induced regulation of gene transcription) and lipogenic genes was evaluated using Pearson’s correlation coefficient. Mice treated with MLE presented with significantly lower body and liver weights as compared with the obese control and MET-treated mice (p ≤ 0.05). Further, MLE treatment significantly inhibited hepatic steatosis as compared with the obese control and MET-treated mice (p ≤ 0.05). The reduced lipid accumulation was associated with low expression of fatty acid synthase (Cpt1; p ≤ 0.05) and an upregulation of the fatty acid oxidation gene, carnitine palmitoyltransferase (Cpt1; p ≤ 0.01), as compared with the obese control mice. Interestingly, MLE treatment improved the correlation between Mthfr and Cpt1 mRNA expression (r = 0.72, p ≤ 0.01). Taken together, the results suggest that Marula leaf extracts may inhibit hepatic steatosis by influencing the association between Mthfr and genes involved in hepatic lipid metabolism. Further studies are warranted to assess DNA methylation changes in lipid metabolism genes.

show abstract

Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

Cited by 9 publications

References 16 publications

Differential Outcomes and Biologic Markers of Radiation-Associated vs. Sporadic Osteosarcoma: A Single-Institution Experience

Differential Outcomes and Biologic Markers of Radiation-Associated vs. Sporadic Osteosarcoma: A Single-Institution Experience

Autophagic tumor-associated macrophages promote the endothelial mesenchymal transition in lung adenocarcinomas through the FUT4/p-ezrin pathway

Sclerocarya birrea (Marula) Extract Inhibits Hepatic Steatosis in db/db Mice

Contact Info

Product

Resources

About