Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma

Abstract

BackgroundAlthough synovial sarcoma is the 3rd most commonly occurring mesenchymal tumor in young adults, usually with a highly aggressive clinical course; remarkable differences can be seen regarding the clinical outcome. According to comparative genomic hybridization (CGH) data published in the literature, the simple and complex karyotypes show a correlation between the prognosis and clinical outcome. In addition, the connection between DNA ploidy and clinical course is controversial. The aim of this study was using a fine-tuning interpretation of our DNA ploidy results and to compare these with metaphase high-resolution CGH (HR-CGH) results.MethodsDNA ploidy was determined on Feulgen-stained smears in 56 synovial sarcoma cases by image cytometry; follow up was available in 46 cases (average: 78 months). In 9 cases HR-CGH analysis was also available.Results10 cases were found DNA-aneuploid, 46 were DNA-diploid by image cytometry. With fine-tuning of the diploid cases according to the 5c exceeding events (single cell aneuploidy), 33 cases were so called "simple-diploid" (without 5c exceeding events) and 13 cases were "complex-diploid"; containing 5c exceeding events (any number). Aneuploid tumors contained large numbers of genetic alterations with the sum gain of at least 2 chromosomes (A-, B- or C-group) detected by HR-CGH. In the "simple-diploid" cases no or few genetic alterations could be detected, whereas the "complex-diploid" samples numerous aberrations (equal or more than 3) could be found.ConclusionsOur results show a correlation between the DNA-ploidy, a fine-tuned DNA-ploidy and the HR-CGH results. Furthermore, we found significant correlation between the different ploidy groups and the clinical outcome (p < 0.05).

Diagnostic and prognostic significance of image cytometric DNA ploidy measurement in cytological samples of cervical squamous intraepithelial lesions

“…Aneuploidy can be stemline or single‐cell aneuploidy 1,4,10,11 . Stemlines with modal values <1.8c, >2.2c and <3.6c or >4.4c reflect stemline aneuploidy.…”

Nuclear DNA-Content in Mesenchymal Lesions in Dogs: Its Value as Marker of Malignancy and Extent of Genomic Instability

“…Many studies in humans [28,30,31,62] reported a similar correlation, although this is not universal [58]. Striking was the absolute lack of a relation between ploidy status and risk of metastasis in OS, which is in contrast to OS in humans [32,63].…”

“…Striking was the absolute lack of a relation between ploidy status and risk of metastasis in OS, which is in contrast to OS in humans [32,63]. In diploid cases within particular types of cancer more detailed cytogenetic analyses are required to discern those changes that predict metastatic behavior [62,64]. …”

Sarcoma synoviale

“…A betegség kiújulására akár 20 év múlva is számítani lehet. Az áttétek predilekciós helyei a következők: tüdő (74-81%), nyirokcsomók (3-23%) és a csont (10-20%) [39].…”