Correlation Between Intestinal Microbiota and Growth of White Shrimp (Litopenaeus Vannamei)

Wang

et al. 2022

Water

The gut microbiota (GM) compositions of aquatic animals are influenced by microorganisms in ambient water and sediment. However, the extent to which environmental microorganisms can affect shrimp GM composition is unknown. We analyzed the impact of water and sediment microorganisms on the GM of Macrobrachium rosenbergii at different growth stages. We collected water, sediment, and M. rosenbergii gut samples at the early, middle, and late stages of an M. rosenbergii culture and analyzed the microbiota composition. The shrimps’ body weight differed significantly between sampling stages. The shrimp GM composition differed significantly from that of the ambient water and sediment, and these differences were remarkably stronger than those between the shrimp GM at different sampling times and in different ponds. The proportion of sediment bacteria in the shrimp GM was approximately three times higher than that of water bacteria, which changed among ponds and over sampling time. These results provide important reference information for a deeper understanding of the impact of environmental microorganisms on the composition of shrimp GM. Moreover, the results also provide reference information for increasing the production of shrimp culture as well as ensuring a good health status of the culture.

Section: Introductionmentioning

confidence: 99%

Effects of Ambient Microbiota on the Gut Microbiota of Macrobrachium rosenbergii

Liu

Wang

et al. 2022

Water

“…Delftia belongs to the order Burkholderiales, and most of its members have biodegradation effects, such as degrading peptidoglycan and aniline [ 36 , 37 ]. Delftia was widespread in rhizosphere soil, activated sludge, and polluted environment [ 38 ], and has been reported to be found in the gut flora of fruit fly ( Bactrocera tau ) and white shrimp ( Litopenaeus vannamei ) [ 39 , 40 ]. In this study, the relative abundance of Raoultella and Delftia in B. calyciflorus -associated bacteria was extremely high but extremely low in the natural environmental water, indicating that they relied on the habitat environment provided by the B. calyciflorus body, with host specificity.…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Brachionus calyciflorus-Associated Bacterial and Bacterioplankton Communities in a Subtropical Freshwater Lake

Zhang

Feng

et al. 2022

Animals

Zooplankton bodies are organic-rich micro-environments that support fast bacterial growth. Therefore, the abundance of zooplankton-associated bacteria is much higher than that of free-living bacteria, which has profound effects on the nutrient cycling of freshwater ecosystems. However, a detailed analysis of associated bacteria is still less known, especially the relationship between those bacteria and bacterioplankton. In this study, we analyzed the relationships between Brachionus calyciflorus-associated bacterial and bacterioplankton communities in freshwater using high-throughput sequencing. The results indicated that there were significant differences between the two bacterial communities, with only 29.47% sharing OTUs. The alpha diversity of the bacterioplankton community was significantly higher than that of B. calyciflorus-associated bacteria. PCoA analysis showed that the bacterioplankton community gathered deeply, while the B. calyciflorus-associated bacterial community was far away from the whole bacterioplankton community, and the distribution was relatively discrete. CCA analysis suggested that many environmental factors (T, DO, pH, TP, PO43-, NH4+, and NO3-) regulated the community composition of B. calyciflorus-associated bacteria, but the explanatory degree of variability was only 37.80%. High-throughput sequencing revealed that Raoultella and Delftia in Proteobacteria were the dominant genus in the B. calyciflorus-associated bacterial community, and closely related to the biodegradation function. Moreover, several abundant bacterial members participating in carbon and nitrogen cycles were found in the associated bacterial community by network analysis. Predictive results from FAPROTAX showed that the predominant biogeochemical cycle functions of the B. calyciflorus-associated bacterial community were plastic degradation, chemoheterotrophy, and aerobic chemoheterotrophy. Overall, our study expands the current understanding of zooplankton–bacteria interaction and promotes the combination of two different research fields.

“…Therefore, the formation and maintenance mechanisms of gut microbiota diversity have attracted much attention and have become a research hotspot in microbial ecology (F. Huang et al, 2018; Ni et al, 2014a, 2014b; Xiong et al, 2017). Although many ecological theories have been developed (such as dispersal limitations, environmental filtering processes, and distance–decay relationships), and widely used in the study of microbial ecology (Chytrý et al, 2012; Moeller et al, 2017; Ni et al, 2014a; Peay et al, 2010), most studies on crustacean gut microbiota have focused on describing the composition of gut microbiota and its relationship with host growth or disease (J. Y. Wu et al, 2021; Yu et al, 2018; Z. Zhang et al, 2020). Our previous studies showed that the proportion of M. rosenbergii gut microbiota from ambient sediment microbiota was significantly higher than that from water microbiota across the entire aquaculture process (Liu et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Gut microbiota plays vital roles in growth and health of shrimp (Holt et al, 2021; Liu et al, 2022; Schleder et al, 2020; J. Y. Wu et al, 2021; Yu et al, 2018). Although extensive studies about the gut microbiota of mammals and terrestrial invertebrates have been conducted, relatively little is known about the bacteria living in the gut of aquatic invertebrates (Holt et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Proportions of Pacific white shrimp, Litopenaeus vannamei, gut microbiota from ambient microbiota increased with aquaculture process

Liu

J World Aquaculture Soc

Wang

et al. 2022

To analyze the effects of ambient microbiota on the gut microbiota of Pacific white shrimp, Litopenaeus vannamei, at different aquaculture stages, shrimp gut and ambient water and sediment microbiota at different aquaculture stages were collected and analyzed through high‐throughput sequencing of 16S rDNA. Our results showed that various gut microbiota alpha‐diversity indices at the late aquaculture stage (LAS) were significantly higher than those at the early aquaculture stage (EAS). The average degree of the co‐occurrence network constructed based on the samples at the EAS was significantly higher than that at the middle aquaculture stage (MAS), and they were both significantly higher than those at the LAS. The proportions of gut microbiota from the water microbiota in the MAS and LAS were significantly higher than those at the EAS, and the proportion of gut microbiota from the sediment microbiota at the LAS was significantly higher than that at the EAS. These results imply that the gut microbiota of shrimp seedlings obtained a small amount of microorganisms from the ambient microbiota and gradually formed new gut microbiota with the inherent microbiota. These results provide support for the subsequent development of aquaculture technology to prevent bacterial diseases in Pacific white shrimp.