Correlation between methylation of the E-Cadherin gene and malignancy of prostate cancer

Abstract

ABSTRACT. Prostate cancer is a common malignant tumor in males with an unclear pathogenic mechanism. As one epigenetic regulation mechanism, DNA methylation of the whole genome and specific gene(s) plays critical roles in pathogenesis, progression, diagnosis, and treatment of prostate cancer. The E-Cadherin gene is involved in cell metabolism and has been suggested to be related with malignancy of multiple tumors. This study investigated the correlation between E-Cadherin methylation and malignancy of prostate cancer. Gradient concentrations of 5-Aza-CdR (5, 10, and 20 mM) were used to treat the prostate cancer cell line (LNCaP), and mRNA level of E-Cadherin was detected by reverse transcription-polymerase chain reaction (RT-PCR). A total of 82 prostate cancer patients were recruited to detect the methylation status of the promoter region of the E-Cadherin gene by pyrophosphate sequencing. Real-time fluorescent quantitative PCR (qRT-PCR) was employed to determine mRNA levels of E-Cadherin. Methylation and mRNA levels of E-Cadherin were analyzed by the SPSS software. With elevated concentrations of 5-Aza-CdR, mRNA levels of E-Cadherin gradually increased. DNA methylation levels of tumor tissues were significantly elevated with increased Gleason score (P < 0.05) and tumor-node-metastasis stage (P < 0.05) but were not related to age, smoking habits, or alcohol consumption (P > 0.05). DNA methylation level was negatively correlated with mRNA expression of the E-Cadherin gene. Methylation in tumor tissues was significantly higher than that in tumor adjacent tissues (P < 0.05). DNA methylation level of the E-Cadherin gene could be an important predictive index for malignancy of prostate cancer.

Lutein combined with EGCG improved retinitis pigmentosa against N-methyl-N nitrosourea-induced

“…29 Our previous study observed that polyphenols affect the bioavailability of carotenoids. 18 In this study, LUT contents were detected in the liver and feces of mice. As shown in Fig.…”

“…The gavage dose of LUT was set at 100 mg per kg (BW per day), 16 the gavage dose of EGCG was set at 500 mg per kg (BW per day), and the ratio between LUT and EGCG was referred from the previous study. 18 LUT was dissolved in soybean oil, and EGCG was dissolved in water to prepare the corresponding concentration solution. The quality of LUT in the LUT-EGCG group is equivalent to that in the LUT and EGCG groups alone.…”

“…Research shows that EGCG significantly reduces the levels of inflammatory markers (such as TNF-α, IL-6, and ICAM-1) to reduce inflammatory responses, thereby protecting the human retinal vascular endothelial cells (HRECs) from the adverse effects of a high-glucose environment. 18 In addition, studies have found that polyphenols (such as EGCG and resveratrol) combined with carotenoids (such as LUT and β-carotene) demonstrated significant synergistic anti-inflammatory effects. 13 However, the synergistic anti-inflammatory effect of LUT and EGCG on improving RP has not been reported yet.…”

E-Cadherin Downregulation is Mediated by Promoter Methylation in Canine Prostate Cancer

“…In human PC, E-cadherin downregulation is frequent in later stages of the disease and poorly differentiated tumors (Ipekci et al, 2015; Zhang et al, 2016a). Considering the dynamic process of E -cadherin expression, a group of cases with negative cells could also be associated with worse prognosis in canine PC.…”

AMD3100 inhibits epithelial–mesenchymal transition, cell invasion, and metastasis in the liver and the lung through blocking the SDF‐1α/CXCR4 signaling pathway in prostate cancer

“…EMT is an important characteristic of human malignant tumors; decreased E‐cadherin and increased vimentin levels are characteristic of the EMT progression in various human cancers, including PC (X. Li et al, ). Previous studies showed that E‐cadherin interacts with various extracellular and intracellular proteins, and its decreased expression is closely related to metastasis (Zhang, Zhang, & Zhang, ). Increased expression of vimentin is observed during metastasis of many human tumors such as breast cancer and PC (Chaw et al, ; Wei et al, ).…”