Correlation between Positive Rate and Number of Biopsy Samples on Urease Test in ChildhoodHelicobacter pyloriInfection

Abstract

To identify the correlation between the number of gastric biopsy samples and the positive rate, we compared the results of urease test using one and three biopsy samples from each 255 children who underwent gastroduodenoscopy at Gyeongsang National University Hospital. The children were divided into three age groups: 0-4, 5-9, and 10-15 yr. The gastric endoscopic biopsies were subjected to the urease test. That is, one and three gastric antral biopsy samples were collected from the same child. The results of urease test were classified into three grades: Grade 0 (no change), 1 (6-24 hr), 2 (1-6 hr), and 3 (<1 hr). The positive rate of urease test was increased by the age with no respect to the number of gastric biopsy samples (one biopsy P = 0.001, three biopsy P < 0.001). The positive rate of the urease test was higher on three biopsy samples as compared with one biopsy sample (P < 0.001). The difference between one and three biopsy samples was higher in the children aged 0-9 yr. Our results indicate that the urease test might be a more accurate diagnostic modality when it is performed on three or more biopsy samples in children.

Epidemiology and Diagnosis of Helicobacter pylori infection

“…The sensitivity increased from 61% using one biopsy to 74% using four antral biopsies [41]. Similar results were observed in children by Seo et al [42].…”

European Registry on the management of Helicobacter pylori infection (Hp-EuReg protocol): The first results of Russian Centers

“…Проведение ЭГДС через 1 мес после завершения лечения только с целью выявления инфекции может быть оправда-но лишь при недоступности и невозможности при-менения неинвазивных методов. При этом важно иметь в виду, что чувствительность БУТ существен-но ниже, чем у неинвазивных методов, и в зависи-мости от количества биоптатов колеблется от 61 до 74% [13,14]. Одной из распространенных ошибок является применение для контроля эффективности эрадика-ции серологического метода.…”

Characterization of Specific IgA Response to Antigenic Determinants ofHelicobacter pyloriUrease Encoded byureAandureBin Children

“…The serum samples of patients with gastric symptoms were divided into three groups based on the results of CLO test as follows: strong, weak, or negative. The gastric endoscopic biopsies of the patients were applied to the CLO test according to a procedure described previously (13). Briefly, a gastric antral biopsy sample was collected and then incubated in 0.3% urea broth (urea 20 g/l, phenol red 0.04 g/l, KH2PO4 0.2 g/l and NaCl 0.5 g/l, pH=6.8).…”