Correlation between seminal fructose level and sperm motility in infertile male in southern part of Rajasthan - A cross-sectional study

Chauhan, Sangita; Kaur, Manjinder; Sharma, Suman; Kurmi, Naren

doi:10.5455/njppp.2020.10.0513320201062020

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“…Our artificial medium contains the substrates such as glucose, fructose, pyruvate and lactate as energy sources, on which sperm motility is mainly dependent, whereas Ham’s F10 medium only has glucose and pyruvate [ 26 ]. In addition, a negative correlation between semen levels of fructose, as the main source of energy for the sperm motility has been confirmed, especially in in vitro conditions [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Quality of testicular spermatozoa improves with changes in composition of culture medium

Gholizadeh,

Khalili,

Maleki

et al. 2023

Basic Clin. Androl.

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Background Spermatozoa retrieved from the testis and epididymis are deprived of the beneficial effects of seminal fluid. Thus applying an artificial medium with normal seminal fluid characteristics, known as artificial seminal fluid (ASF), may provide an appropriate condition for improving some sperm parameters in azoospermia. The objective was to investigate the impact of in vitro exposure of testicular and epididymal spermatozoa to ASF on sperm quality. The study was conducted on testicular (n = 20) and epididymal (n = 20) sperm specimens obtained from azoospermic men. Each sample was divided into two equal parts: Part I) for processing and incubation with Ham’s F10 medium; Part II) for processing and incubation with ASF. Results After 2 h incubation, testicular sperm motility was significantly higher in ASF than in Ham’s F10 medium. In comparison to 0 h, mitochondrial membrane potential levels of testicular spermatozoa were significantly higher after 2 h and 24 h in ASF and after 24 h in Ham’s F10 medium. Furthermore, the data indicated significantly lower rates of epididymal spermatozoa with high MMP in both media after 24 h. There were no significant differences in the DNA fragmentation index of testicular and epididymal spermatozoa between ASF and Ham’s F10 medium at different time points. Conclusion The results demonstrated that in vitro incubation of testicular spermatozoa improved their motility more effectively than Ham’s F10 medium in the short term (2 h), but had no effect on epididymal spermatozoa. Since the physiology of testicular spermatozoa is different from that of ejaculated spermatozoa, it seems that a special environment should be designed and used for each of them.

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Section: Discussionmentioning

confidence: 99%