Correlation between silver-stained nucleolar organizer region area and cell cycle time

Canet, Véronique; Montmasson, Marie-Paule; Usson, Yves; Giroud, Françoise; Brugal, G.

doi:10.1002/1097-0320(20010201)43:2<110::aid-cyto1025>3.0.co;2-7

Cited by 28 publications

(19 citation statements)

References 20 publications

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“…Some nuclear proteins are associated with NORs and are stained black with silver methods (AgNOR proteins or AgNORs) [22]. It has been reported that AgNORs are related to the rate of cell proliferative activity [33]. Therefore, the decrease of AgNOR dots in groups treated with bioactive compounds over a long term (garlic, tomatoes, and garlic + tomato) shows that these products reduce the proliferative activity of experimental tumors.…”

Section: Discussionmentioning

confidence: 99%

Long‐Term Treatment with Aqueous Garlic and/or Tomato Suspensions Decreases Ehrlich Ascites Tumors

Bom

Gunutzmann

Hurtado

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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We evaluated the preventive and therapeutic effects of aqueous suspensions of garlic, tomato, and garlic + tomato in the development of experimental Ehrlich tumors in mice. The aqueous suspensions (2%) were administered over a short term for 30 days before tumor inoculation and 12 days afterward, and suspensions at 6% were administered for 180 days before inoculation and for 12 days afterward. The volume, number, and characteristics of the tumor cells and AgNOR counts were determined to compare the different treatments. Aqueous 6% suspensions of garlic, tomato, and garlic + tomato given over the long term significantly reduced tumor growth but when given over the short term, they did not alter tumor growth.

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Section: Discussionmentioning

confidence: 99%

Long‐Term Treatment with Aqueous Garlic and/or Tomato Suspensions Decreases Ehrlich Ascites Tumors

Bom

Gunutzmann

Hurtado

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…A high correlation with the number of AgNORs and the granule area was observed ( r = 0.53, P ≤ 0.01) [56]. For cell cultures with a high correlation between MIB-1 and AgNOR expression, a conclusion was made that a combination of these two markers, with the calculation of the proliferating cell pool, will be useful for measuring the cell proliferation rate in normal and malignant tissues [24]. The same conclusion on the correlation between AgNOR and other markers of cellular proliferation (MIB-1, PCNA, BrdU-labeling, and rRNA transcription activity) was made by another group of researchers [57].…”

Section: Discussionmentioning

confidence: 99%

“…The number of AgNORs during interphase is strictly connected with rDNA transcription activity and upregulates this process during continuous cell proliferation [20, 21]. Therefore, the assessment of AgNOR activity makes it possible not only to detect the fraction of proliferating cells but also to assess the rate of proliferation [22–24]. In addition, there is evidence that the number of AgNORs may be an indicator of cell maturity [25].…”

Section: Introductionmentioning

confidence: 99%

Prognostic Value of Microvessel Density in Tumor and Peritumoral Area as Evaluated by CD31 Protein Expression and Argyrophilic Nucleolar Organizer Region Count in Endothelial Cells in Uterine Leiomyosarcoma

Avdalyan

Бобров

Климачев

et al. 2012

Sarcoma

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The objective of this study was to investigate the prognostic value of microvessel density (MVD) in uterine leiomyosarcoma (LMS) and peritumoral area (PA) as evaluated by CD31 expression and argyrophilic nucleolar organizer region (AgNOR) count in endothelial cells. Tissue specimens from 66 patients with uterine LMS were examined. There were no significant differences in the mean MVD between tumor itself and the PA (P = 0.9); moreover, the MVD in the PA often exceeded that in the tumor. No correlation or significant differences were also found in the MVD between different grades of malignancy of LMS (r = 0.1; P = 0.07). The number of AgNORs in tumor endothelial cells was significantly higher in tumor vessels than in the peritumoral area (P < 0.005) and increased with the tumor grade. Analysis of the prognostic value of MVD in uterine LMS and PA showed that the density of tumor vessels was not an independent criterion, while the MVD in the PA affected 10-year survival to a significantly greater extent (χ 2 = 27.5; P = 0.0003). The number of AgNORs also had an important effect on survival of LMS patients: when the threshold of 11.6 granules was exceeded, prognosis was significantly more unfavorable than that prior to exceeding the threshold.

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“…The reason for studying nucleolar organizer region‐associated proteins was that they could act as a marker of ribosomal DNA and possibly of its level of transcription 2 and could provide information about nucleolar activity in hyperplastic and neoplastic states. Recent studies support the hypothesis that cell cycle time and size of the ribogenesis machinery are coregulated so measurement of silver‐stained nucleolar organizer region‐associated proteins (AgNORs) could be used as a static evaluation of cell cycle duration 3 and potentially provide prognostic information.…”

mentioning

confidence: 93%

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Walker¹

2002

Histopathology

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Correlation between silver-stained nucleolar organizer region area and cell cycle time

Cited by 28 publications

References 20 publications

Long‐Term Treatment with Aqueous Garlic and/or Tomato Suspensions Decreases Ehrlich Ascites Tumors

Long‐Term Treatment with Aqueous Garlic and/or Tomato Suspensions Decreases Ehrlich Ascites Tumors

Prognostic Value of Microvessel Density in Tumor and Peritumoral Area as Evaluated by CD31 Protein Expression and Argyrophilic Nucleolar Organizer Region Count in Endothelial Cells in Uterine Leiomyosarcoma

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