Correlation between the chemical composition and the antimicrobial properties of seven samples of essential oils of endemic Thymes in Morocco against multi-resistant bacteria and pathogenic fungi

Drioiche, Aziz; Radi, Fatima Zahra; Ailli, Atika; Bouzoubaa, Amal; Boutakioute, Amale; Mekdad, Soumia; Kamaly, Omkulthom Al; Saleh, Asmaa; Maouloua, Mohamed; Bousta, Dalila; Şahpaz, Sevser; Makhoukhi, Fadoua El; Zair, Touriya

doi:10.1016/j.jsps.2022.06.022

Cited by 18 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another study identified thymol as the main component (43%), with carvacrol and p-cymene as the next most important compounds (Coimbra et al, 2022b). The study on Moroccan Thymus zygis L. from the Ifran region edited by Drioiche et al (2022) also revealed a predominance of carvacrol in the essential oil.…”

Section: Gc/msmentioning

confidence: 95%

Phytochemical Investigation of Thymus zygis L. and Salvia officinalis L. Collected from Fez-Meknes Region, Morocco

Benabderrahmane

Atmani²,

Boutagayout³

et al. 2023

Egypt. J. Bot.

View full text Add to dashboard Cite

Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 2215T HYMUS and Salvia are among the most popular plants both in traditional medicine and in the culinary arts. This study has the aims to detect the chemical composition of the extracts and the powder of Thymus zygis L. (T) and Salvia officinalis L. (S) collected from the Fez-Meknes region. Two extracts were prepared: aqueous and essential oil. Phytochemical tests were performed to qualitatively evaluate the presence or absence of phytoconstituents using standard methods. The essential oils were analyzed by gas chromatography-mass spectrometry (GC/MS). Two powder analyses were performed: Fourier transform infrared spectroscopy (FTIR) analysis and elemental analysis. The drying of both plants took a similar amount of time with a noticeable loss in weight for Salvia. The phytochemical screening revealed the abundant presence of terpenoids, catechic tannins, steroids and sterols in the two plants. GC/MS analysis showed richness in carvacrol for Thymus zygis L. and in thujone for Salvia officinalis L. The analysis by FTIR showed characteristic peak readings of various functional groups in the powders, citing proteins, aliphatic compounds, carbonyl compounds and aromatic rings. In the elemental analysis, there is a high carbon content for Thymus and Salvia

show abstract

Section: Gc/msmentioning

confidence: 95%

Phytochemical Investigation of Thymus zygis L. and Salvia officinalis L. Collected from Fez-Meknes Region, Morocco

Benabderrahmane

Atmani²,

Boutagayout³

et al. 2023

Egypt. J. Bot.

View full text Add to dashboard Cite

show abstract

“…These variabilities confirm that the chemical profile highly depends on the origin of the plant, climate factors, genetic inheritance, abiotic factors, etc. [44].…”

Section: Gc and Gc/ms Analysis Of Tzeo Volatile Compositionmentioning

confidence: 99%

Thymus zygis, Valuable Antimicrobial (In Vitro and In Situ) and Antibiofilm Agent with Potential Antiproliferative Effects

Vukić,

Čmiková,

Hsouna

et al. 2023

Plants

View full text Add to dashboard Cite

With the growing issues of food spoilage, microbial resistance, and high mortality caused by cancer, the aim of this study was to evaluate T. zygis essential oil (TZEO) as a potential solution for these challenges. Here, we first performed GC/MS analysis which showed that the tested TZEO belongs to the linalool chemotype since the abundance of linalool was found to be 38.0%. Antioxidant activity assays showed the superiority of TZEO in neutralizing the ABTS radical cation compared to the DPPH radical. The TZEO was able to neutralize 50% of ABTS•+ at the concentration of 53.03 ± 1.34 μg/mL. Antimicrobial assessment performed by employing disc diffusion and minimal inhibitory concentration assays revealed TZEO as a potent antimicrobial agent with the highest inhibition activity towards tested gram-negative strains. The most sensitive on the treatment with TZEO was Enterobacter aerogenes showing an MIC 50 value of 0.147 ± 0.006 mg/mL and a MIC 90 value of 0.158 ± 0.024 mg/mL. Additionally, an in situ analysis showed great effects of TZEO in inhibiting gram-negative E. coli, P. putida, and E. aerogenes growing on bananas and cucumbers. Treatment with the TZEO vapor phase in the concentration of 500 μg/mL was able to reduce the growth of these bacteria on the food models to the extent > 90%, except for E. coli growth on the cucumber, which was reduced to the extent of 83.87 ± 4.76%. Furthermore, a test on the antibiofilm activity of the tested essential oil revealed its biofilm prevention effects against Salmonella enterica which forms biofilms on plastic and stainless-steel surfaces. Performed tests on the TZEO effects towards cell viability showed no effects on the normal MRC-5 cell line. However, the results of MTT assay of TZEO effects on three cancer cell lines (MDA-MB-231, HCT-116, and K562) suggest that TZEO exerted the strongest effects on the inhibition of the viability of MDA-MB-231 cells, especially after long-term treatment in the highest concentration applied with reducing the viability of the cells to 57%. Additionally, results of NBT and Griess assays suggest that TZEO could be a convenient candidate for future testing for developing novel antitumor therapies.

show abstract

“…It is a perennial shrub that grows between 10 and 60 cm in height and is found exclusively in semiarid regions of the Moroccan High Atlas and Anti-Atlas. Locally, it is referred to as “Zaitra” or “Azkuni” [ 3 , 4 , 5 ]. Traditional uses of this plant include the treatment of various conditions such as diabetes, hypertension, fever, skin and blood circulation disorders, bronchitis, pain perception, immune system issues, pharyngitis, and influenza [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Thymus satureioides Coss.: Mineral Composition, Nutritional Value, Phytochemical Profiling, and Dermatological Properties

et al. 2023

View full text Add to dashboard Cite

Zaitra, Thymus satureioides, is an aromatic plant with a long history of use in traditional medicine. In this study, we assessed the mineral composition, nutritional value, phytocontents, and dermatological properties of the aerial parts of T. satureioides. The plant contained high contents of calcium and iron, moderate levels of magnesium, manganese, and zinc, and low contents of total nitrogen, total phosphorus, total potassium, and copper. It is rich in several amino acids, including asparagine, 4-hydroxyproline, isoleucine, and leucine, and the essential amino acids account for 60.8%. The extract contains considerable amounts of polyphenols and flavonoids (TPC = 118.17 mg GAE/g extract and TFC = 32.32 mg quercetin/g extract). It also comprises 46 secondary metabolites, identified through LC-MS/MS analysis, belonging to phenolic acids, chalcones, and flavonoids. The extract elicited pronounced antioxidant activities, inhibited the growth of P. aeruginosa (MIC = 50 mg/mL), and reduced biofilm formation by up to 35.13% using the ¼ sub-MIC of 12.5 mg/mL. Moreover, bacterial extracellular proteins and exopolysaccharides were diminished by 46.15% and 69.04%, respectively. Likewise, the swimming of the bacterium was impaired (56.94% decrease) in the presence of the extract. In silico, skin permeability and sensitization effects revealed that out of the 46 identified compounds, 33 were predicted to be exempt from any skin sensitivity risk (Human Sensitizer Score ≤ 0.5), while extensive skin permeabilities were observed (Log Kp = −3.35–−11.98 cm/s). This study provides scientific evidence about the pronounced activities of T. satureioides, supports its traditional uses, and promotes its utilization in the development of new drugs, food supplements, and dermatological agents.

show abstract

Correlation between the chemical composition and the antimicrobial properties of seven samples of essential oils of endemic Thymes in Morocco against multi-resistant bacteria and pathogenic fungi

Cited by 18 publications

References 42 publications

Phytochemical Investigation of Thymus zygis L. and Salvia officinalis L. Collected from Fez-Meknes Region, Morocco

Phytochemical Investigation of Thymus zygis L. and Salvia officinalis L. Collected from Fez-Meknes Region, Morocco

Thymus zygis, Valuable Antimicrobial (In Vitro and In Situ) and Antibiofilm Agent with Potential Antiproliferative Effects

Thymus satureioides Coss.: Mineral Composition, Nutritional Value, Phytochemical Profiling, and Dermatological Properties

Contact Info

Product

Resources

About