Correlation Between the Concentrations of Prostaglandin Dehydrogenase and Progesterone in the Early Human Placenta

Falkay, George; M, Sas

doi:10.1677/joe.0.0760173

Cited by 22 publications

(5 citation statements)

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“…13,14-Dihydro-15-keto- (5,6,8,9,11,12,14(n)-3 H)-PGF 2␣ (125 Ci/mmol, 1 mCi/ml), (5,6,8,9,11,12,14,15(n)-3 H)-PGF 2␣ (160 Ci/mmol, 200 uCi/ml) and 17␣-hydroxy-(1,2,6,7-3 H-progesterone (60 Ci/mmol, 1 mCi/ml) were obtained from Amersham Corporation (Arlington Heights, IL, USA).…”

Section: Drugs and Chemicalsmentioning

confidence: 99%

“…In the feto-placental unit, PGDH has been implicated in the maintenance of pregnancy, as high PGDH activity in the chorion protects the myometrium from the high levels of PGF 2␣ produced by the amnion [8][9][10]. A number of correlations between PGDH enzyme activity and progesterone levels in human placental and uterine tissues have led to the suggestion that the enzyme is under steroidal control [11][12][13]. For example, the lungs of pregnant rabbits increase expression of PGDH in response to progesterone [14] perhaps to provide protection of the corpora lutea of pregnancy from circulating PGF 2␣ [15].…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis and catabolism of prostaglandin F2(alpha) (PGF2α) are controlled by progesterone in the rat uterus during pregnancy

Farina

Ribeiro

Weissmann

et al. 2004

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Drugs and Chemicalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biosynthesis and catabolism of prostaglandin F2(alpha) (PGF2α) are controlled by progesterone in the rat uterus during pregnancy

Farina

Ribeiro

Weissmann

et al. 2004

The Journal of Steroid Biochemistry and Molecular Biology

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“…Immunohistochemical studies and enzymatic assays in human uterine tissues of the cycle and early pregnancy suggested a stimulatory effect of progesterone on PGDH (21,54). In the human endometrium, PGDH protein levels are higher in secretory than in proliferative endometrium (22), and treatment with antiprogestins leads to a reduction of PG catabolism.…”

Section: Cox-2 and Pgdh Expression In Guinea Pig Endometriummentioning

confidence: 99%

“…In the feto-placental unit, PGDH has been implicated in the maintenance of pregnancy, as high PGDH activity in the chorion protects the myometrium from the high levels of PGF 2␣ produced by the amnion (18 -20). A number of correlations between PGDH enzyme activity and progesterone levels in human placental and uterine tissues have led to the suggestion that the enzyme is under steroidal control (21)(22)(23). It can be inferred therefore that the COX isoenzymes and PGDH in concert determine the level of biologically active prostaglandins.…”

mentioning

confidence: 99%

Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2 Secretion in Cultured Endometrial Cells

Bracken¹

1997

Endocrinology

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Prostaglandin F 2␣ (PGF 2␣ ) secretion is lowest at midcycle and highest on day 15 at luteolysis in the cycling guinea pig uterus and is inversely related to serum progesterone levels. An increase in 17-␤ estradiol (E 2 ) occurs only towards the end of the cycle. To investigate the effect of steroids on the control of uterine PGF 2␣ metabolism at the level of gene expression we established a primary cell culture model of day 15 cycling guinea pig endometrial cells. We cloned guinea pig cDNAs for cyclooxygenase 2 (COX-2), 15-hydroxyprostaglandin dehydrogenase (PGDH) that converts PGF 2␣ to biologically inactive 13,14-dihydro-15-keto PGF 2␣ (PGFM) and a fragment of cyclooxygenase-1 (COX-1). They were found to bear 87% and 90% homology at the amino acid level to their human counterparts for COX-2 and PGDH, respectively, retaining all functional sites. Purified epithelial and stromal cell subcultures were primed with medium containing either E 2 or medroxyprogesterone acetate (MPA) for 24 h. They were then treated for a further 4 or 24 h either withdrawing the steroid, maintaining the priming steroid, or supplementing with both steroids, before harvesting conditioned media and RNA. Epithelial cells secreted 30-fold more PGF 2␣ compared with stromal cells (e.g. 7.8 Ϯ 0.7 vs. 0.26 Ϯ 0.09 pg/ng DNA•24 h), and PGF 2␣ secretion levels were approximately 15-fold higher than those of PGFM (e.g. 7.8 Ϯ 0.7 vs. 0.45 Ϯ 0.16 pg/ng DNA⅐24 h, for epithelial cells). COX-1 transcripts were low and unaffected by treatment in both cell types. COX-2 transcripts were more abundant in epithelial than stromal cells. Steroid-modulated, COX-2 dependent changes in PGF 2␣ secretion were observed. The addition of MPA to E 2 primed cells caused a decrease in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 h. Conversely, the addition of E 2 to MPA primed epithelial cells led to an increase in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 and 24 h. The withdrawal of E 2 caused a fall in PGF 2␣ secretion and COX-2 transcripts after 24 h. In contrast, PGDH transcripts were more abundant in stromal than epithelial cells and were up-regulated by the addition of MPA to E 2 primed cells. These in vitro observations are in keeping with the secretory profile seen in vivo in the cycling guinea pig uterus suggesting that 1) the fall of E 2 and the coinciding rise in progesterone seen in the early cycle lead to a reduction in PGF 2␣ levels; and 2) the rise of E 2 in the late cycle on a progesterone primed uterus is the stimulus for an increase in uterine PGF 2␣ production. Our findings suggest a differential role for uterine stroma and epithelium in vivo whereby the former acts to remove (via PGDH), and the latter to produce (via COX-2) biologically active prostaglandin. (Endocrinology 138: [237][238][239][240][241][242][243][244][245][246][247] 1997)

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“…The levels of catechol oestrogens used in these studies may appear high but the catechol oestrogens are liable to oxidation both enzymically (Nelson, Mitchell, Dybing & Sasame, 1976) and non-enzymically (Gelbke & Knuppen, 1972) (Alam, Russell, Tabor & Moulton, 1976;Falkay & Sas, 1978).…”

Section: Male Reproductive Tractmentioning

confidence: 99%

Prostaglandin synthesis in the male and female reproductive tract

Kelly¹

1981

Reproduction

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Correlation Between the Concentrations of Prostaglandin Dehydrogenase and Progesterone in the Early Human Placenta

Cited by 22 publications

References 0 publications

Biosynthesis and catabolism of prostaglandin F2(alpha) (PGF2α) are controlled by progesterone in the rat uterus during pregnancy

Biosynthesis and catabolism of prostaglandin F2(alpha) (PGF2α) are controlled by progesterone in the rat uterus during pregnancy

Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2 Secretion in Cultured Endometrial Cells

Prostaglandin synthesis in the male and female reproductive tract

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