Correlation between the expression of S100A4 and the efficacy of TAC neoadjuvant chemotherapy in breast cancer

Li, Wen-lei; Zhang, Yang; Liu, Bao‐Guo; Du, Qian; Zhou, Chang‐Xin; Tian, Xingsong

doi:10.3892/etm.2015.2743

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…It can support tumorigenesis by triggering angiogenesis. Recently, S100A4 has attracted the attention of researchers because its expression is related to the movement, invasion, metastasis, angiogenesis and prognosis of various tumors [ 42 ], which agree well with our findings. Our study also proved that RI inhibited ILK expression and suppressed angiogenesis both in vivo and in vitro.…”

Section: Discussionsupporting

confidence: 91%

Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways

Zhuang

Zhong

et al. 2016

J Exp Clin Cancer Res

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BackgroundIntegrin-linked kinase (ILK) is a multifunctional adaptor protein which is involved with protein signalling within cells to modulate malignant (cancer) cell movement, cell cycle, metastasis and epithelial–mesenchymal transition (EMT). Our previous experiment demonstrated that ILK siRNA inhibited the growth and induced apoptosis of bladder cancer cells as well as increased the expression of Ribonuclease inhibitor (RI), an important cytoplasmic protein with many functions. We also reported that RI overexpression inhibited ILK and phosphorylation of AKT and GSK3β. ILK and RI gene both locate on chromosome 11p15 and the two genes are always at the adjacent position of same chromosome during evolution, which suggest that ILK and RI could have some relationship. However, underlying interacting mechanisms remain unclear between them. Here, we postulate that RI might regulate ILK signaling pathway via interacting with ILK.MethodsCo-immunoprecipitation, GST pull-down and co-localization under laser confocal microscope assay were used to determine the interaction between ILK and RI exogenously and endogenously. Furthermore, we further verified that there is a direct binding between the two proteins by fluorescence resonance energy transfer (FRET) in cells. Next, The effects of interplay between ILK and RI on the key target protein expressions of PI3K/AKT/mTOR signaling pathway were determined by western blot, immunohistochemistry and immunofluorescence assay in vivo and in vitro. Finally, the interaction was assessed using nude mice xenograft model.ResultsWe first found that ILK could combine with RI both in vivo and in vitro by GST pull-down, co-immunoprecipitation (Co-IP) and FRET. The protein levels of ILK and RI revealed a significant inverse correlation in vivo and in vitro. Subsequently, The results showed that up-regulating ILK could increase cell proliferation, change cell morphology and regulate cell cycle. We also demonstrated that the overexpression of ILK remarkably promoted EMT and expressions of target molecules of ILK signaling pathways in vitro and in vivo. Finally, we found that ILK overexpression significantly enhanced growth, metastasis and angiogenesis of xenograft tumor; Whereas, RI has a contrary role compared to ILK in vivo and in vitro.ConclusionsOur findings, for the first time, directly proved that the interplay between ILK and RI regulated EMT via ILK/PI3K/AKT signaling pathways for bladder cancer, which highlights the possibilities that ILK/RI could be valuable markers together for the therapy and diagnosis of human carcinoma of urinary bladder.

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Section: Discussionsupporting

confidence: 91%

Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways

Zhuang

Zhong

et al. 2016

J Exp Clin Cancer Res

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A proteomic glimpse into the oncogenesis of prostate cancer

et al. 2018

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Prostate cancer (PCa) is the second most frequent cancer in men worldwide. Distinguishing between the nonaggressive and aggressive forms of this disease is difficult, and a means to better characterize molecular patterns that could aid in diagnosis is urgently needed. Here, we compare the proteomic profiles of PCa and benign prostatic hyperplasia (BPH) in an effort to elucidate underlying mechanisms of oncogenesis. We compared protein expression in PCa and BPH tissue biopsies using quantitative tandem mass tag (TMT) and a MultiNotch data acquisition proteomic on an Orbitrap Fusion. Four proteins that were observed to be differentially abundant in the mass spectrometry analysis were selected for further comparison with quantitative real-time PCR: S100A4, L-lactate dehydrogenase B-chain (LDHB), Phosphatidylethanolamine-binding-protein 1-RAF (RKIP), and Ras suppressor-protein-1 (RSU1). Mass spectrometry showed RKIP and RSU1 to be upregulated in PCa, while S100A4 and LDHB were upregulated in BPH. q-PCR results were in agreement with quantitative proteomic data in BPH tissue but disagree with gene expression analysis of PCa samples. These four elements showed higher gene expression in BPH than in PCa. Taken together, our results complement and reinforce the current understanding of prostate cancer progression.

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FGFR‑related phenotypic and functional profile of CAFs in prognostication of breast cancer (Review)

Solek,

Braun,

Sadej

et al. 2024

Int J Oncol

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Correlation between the expression of S100A4 and the efficacy of TAC neoadjuvant chemotherapy in breast cancer

Cited by 3 publications

References 22 publications

Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways

Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways

A proteomic glimpse into the oncogenesis of prostate cancer

FGFR‑related phenotypic and functional profile of CAFs in prognostication of breast cancer (Review)

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