Correlation between the extent of platelet activation in platelet concentrates and <i>in vitro</i> and <i>in vivo</i> parameters

Dijkstra‐Tiekstra, Margriet J.; Pietersz, R. N. I.; Huijgens, Peter C.

doi:10.1111/j.1423-0410.2004.00573.x

Cited by 40 publications

(50 citation statements)

References 19 publications

(30 reference statements)

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“…Subjects studied: Whereas several studies have been performed concerning the functional and quality properties of BCs and PRP-PCs [6,8,5]. Heaton et al [4] compared components prepared by the PRP-PC and BC methods.…”

Section: Platelet Preparationmentioning

confidence: 99%

“…We here report on the difference between both platelet concentrates in functional properties of platelet in BC and PRP-PC are compared over storage of 5 days. Processing and storage may affect both platelet morphology and function and they may thus be less effective when transfused than fresh platelets [1,[3][4][8][9]. There are several methods for quality control of platelet components including cell counting, pH, swirling and markers for platelet activation [8,[10][11].…”

Section: Introductionmentioning

confidence: 99%

“…This essay is based on the principle that annexin V binds to PS, with high affinity and high specificity [8,[12][13][14]. Activation of thrombocytes is followed by an increased CD62P expression; this protein is the main component of the alpha granules secreted from the granules during storage [7][8]. The aim of this study is to characterize the quality of the changes occurred in the platelet activation parameters …”

Section: Introductionmentioning

confidence: 99%

“…Processing and storage may affect both platelet morphology and function and they may thus be less effective when transfused than fresh platelets [1,[3][4][8][9]. There are several methods for quality control of platelet components including cell counting, pH, swirling and markers for platelet activation [8,[10][11]. Redistribution of phosphatidylserine (PS) from the inner membrane surface to the exterior surface accompanies during platelet activation [11,12,13].…”

Section: Introductionmentioning

confidence: 99%

“…The exposure of PS can be measured by using flow cytometry and fluorescent-labeled annexin V. Annexin V was used as a parameter for quality monitoring of platelet concentrates during storage. This essay is based on the principle that annexin V binds to PS, with high affinity and high specificity [8,[12][13][14]. Activation of thrombocytes is followed by an increased CD62P expression; this protein is the main component of the alpha granules secreted from the granules during storage [7][8].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

Ali¹

2011

J Blood Disord Transfus

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Section: Platelet Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

Ali¹

2011

J Blood Disord Transfus

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Bispecific Antibody Inhalation Therapy for Redirecting Stem Cells from the Lungs to Repair Heart Injury

et al. 2020

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Stem cell therapy is a promising strategy for cardiac repair. However, clinical efficacy is hampered by poor cell engraftment and the elusive repair mechanisms of the transplanted stem cells. The lung is a reservoir of hematopoietic stem cells (HSCs) and a major biogenesis site for platelets. A strategy is sought to redirect lung resident stem cells to the injured heart for therapeutic repair after myocardial infarction (MI). To achieve this goal, CD34-CD42b platelet-targeting bispecific antibodies (PT-BsAbs) are designed to simultaneously recognize HSCs (via CD34) and platelets (via CD42b). After inhalation delivery, PT-BsAbs reach the lungs and conjoined HSCs and platelets. Due to the innate injury-finding ability of platelets, PT-BsAbs guide lung HSCs to the injured heart after MI. The redirected HSCs promote endogenous repair, leading to increased cardiac function. The repair mechanism involves angiomyogenesis and inflammation modulation. In addition, the inhalation route is superior to the intravenous route to deliver PT-BsAbs in terms of the HSCs' homing ability and therapeutic benefits. This work demonstrates that this novel inhalable antibody therapy, which harnesses platelets derived from the lungs, contributes to potent stem cell redirection and heart repair. This strategy is safe and effective in a mouse model of MI.

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Platelet‐rich plasma‐loaded chitosan scaffolds: Preparation and growth factor release kinetics

et al. 2012

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The aim of this study is to compare the effects of different platelet-rich plasma (PRP) preparation methods on platelet activity and to investigate the growth factor (GF) release kinetics from PRP-loaded chitosan scaffolds for tissue engineering applications. Flow cytometry analysis showed that centrifugation processes used for PRP preparation did not cause significant effect on platelet activation levels by means of markers investigated. Two different methods were used to prepare PRP-loaded chitosan scaffolds: (i) PRP was added to chitosan gel before freeze-drying to prepare scaffolds called as "GEL" and (ii) PRP was embedded to freeze-dried chitosan scaffolds to prepare scaffolds called as "SPONGE." In addition, nonactivated PRP and PRP activated with type-I collagen were used as control groups. Scanning electron microscopy images demonstrated that, in GEL group, there is no deterioration on the scaffolds porous, 3D, and interconnected structure. GF release kinetics was determined by enzyme-linked immunosorbent assay for platelet-derived GF-BB, transforming GF-β1, and insulin-like GF-1. A sustained release of GFs was achieved in GEL group while a sharp burst release was observed for all the GFs from the SPONGE groups. Moreover, platelet-derived GF-BB, insulin-like GF-1, and transforming GF-β1 releases were prolonged to 20 days in GEL groups, and the biological activities of all GFs released from GEL and SPONGE scaffolds were preserved. This study demonstrated that chitosan scaffold that was called GEL could be an appropriate carrier for PRP applications by providing sustained release of GFs.

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Correlation between the extent of platelet activation in platelet concentrates and in vitro and in vivo parameters

Cited by 40 publications

References 19 publications

Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

Bispecific Antibody Inhalation Therapy for Redirecting Stem Cells from the Lungs to Repair Heart Injury

Platelet‐rich plasma‐loaded chitosan scaffolds: Preparation and growth factor release kinetics

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