Correlation between the loss of intracellular molecules and cell viability after cell electroporation

Jakštys, Baltramiejus; Jakutavičiūtė, Milda; Uzdavinyte, Dovile; Šatkauskienė, Ingrida; Šatkauskas, Saulius

doi:10.1016/j.bioelechem.2020.107550

Cited by 29 publications

(15 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, the factors released from irreversibly electroporated cells presumably play several roles, including promotion of cell viability and enhancement of cell migration. Similar results were obtained by our recent study, where we showed that some irreversibly electroporated cells can be rescued by supplementing the medium with compounds obtained from irreversibly electroporated cells [ 24 ]. We determined that the intracellular molecules that contribute to the increase in cell viability are larger than 30 kDa.…”

Section: Discussionsupporting

confidence: 90%

“…Interestingly, the rate and magnitude of the IRE-induced release of DAMPs, namely ATP and HMGB1, were reported to be much greater than those induced by other treatment modalities, such as ionizing radiation and chemotherapy [ 21 ]. Our previous study also demonstrated that cell death following cell electroporation is at least partially related to leakage of intracellular molecules [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation

et al. 2021

View full text Add to dashboard Cite

One of current applications of electroporation is electrochemotherapy and electroablation for local cancer treatment. Both of these electroporation modalities share some similarities with radiation therapy, one of which could be the bystander effect. In this study, we aimed to investigate the role of the bystander effect following these electroporation-based treatments. During direct CHO-K1 cell treatment, cells were electroporated using one 100 µs duration square wave electric pulse at 1400 V/cm (for bleomycin electrotransfer) or 2800 V/cm (for irreversible electroporation). To evaluate the bystander effect, the medium was taken from directly treated cells after 24 h incubation and applied on unaffected cells. Six days after the treatment, cell viability and colony sizes were evaluated using the cell colony formation assay. The results showed that the bystander effect after bleomycin electrotransfer had a strong negative impact on cell viability and cell colony size, which decreased to 2.8% and 23.1%, respectively. On the contrary, irreversible electroporation induced a strong positive bystander effect on cell viability, which increased to 149.3%. In conclusion, the results presented may serve as a platform for further analysis of the bystander effect after electroporation-based therapies and may ultimately lead to refined application of these therapies in clinics.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The damage caused to the tissue by EP-related therapies can elicit ICD and promote a proinflammatory milieu and the recruitment of antigen presenting cells (APC) where tumor-specific antigens (TSA) and tumor associated antigens (TAA) might be captured and presented by APCs to T cells, leading to adaptive immune responses [ 112 , 113 , 114 , 115 , 116 , 117 ]. In fact, RE alone or in combination with bleomycin can lead to the leakage of cytoplasmatic molecules related to ICD and the activation of proinflammatory markers [ 114 , 118 , 119 , 120 ]. The main immunostimulatory molecules related to RE include: adenosine triphosphate (ATP) release, calreticulin (CRT) translocation to cell membrane, release of the non-histone, protein high mobility group box 1 (HMGB1), induction of heat shock protein family (HSP70) stress response and other proinflammatory genes [ 114 , 121 , 122 ].…”

Section: Immunological Aspects Of Reversible Electroporation and Electrochemotherapymentioning

confidence: 99%

Clinical Applications and Immunological Aspects of Electroporation-Based Therapies

et al. 2021

View full text Add to dashboard Cite

Reversible electropermeabilization (RE) is an ultrastructural phenomenon that transiently increases the permeability of the cell membrane upon application of electrical pulses. The technique was described in 1972 by Neumann and Rosenheck and is currently used in a variety of applications, from medicine to food processing. In oncology, RE is applied for the intracellular transport of chemotherapeutic drugs as well as the delivery of genetic material in gene therapies and vaccinations. This review summarizes the physical changes of the membrane, the particularities of bleomycin, and the immunological aspects involved in electrochemotherapy and gene electrotransfer, two important EP-based cancer therapies in human and veterinary oncology.

show abstract

“…When the cells loaded with the indicator are electroporated with the interest ions, fluorescence increase can be observed using a fluorescence microscope or flow cytometry. Monitoring the extracellular release of membrane-impermeable intracellular molecules can detect electroporation [ 28 , 29 , 30 ]. The efflux of cell-impermeable small molecules can be analyzed by appropriate methods.…”

Section: Introductionmentioning

confidence: 99%

Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers

Watanabe

Nihonyanagi

Shibata

et al. 2022

Sensors

View full text Add to dashboard Cite

Membrane permeabilization stimulated by high-voltage electric pulses has been used to deliver cell-impermeable exogenous molecules. The electric field effect on the cells depends on various experimental parameters, such as electric field strength, the number of electric pulses, and the electroporation medium. In this study, we show the influence of the electroporation medium on membrane permeabilization stimulated by electrical short-circuiting via an aqueous droplet in dielectric oil, a novel methodology developed by our previous investigations. We investigated the membrane permeabilization by three methods, influx of calcium ions, uptake of nucleic acid-binding fluorophores (YO-PRO-1), and calcein leakage. We demonstrated that the external medium conductivity had a significant impact on the cells in all described experiments. The short-circuiting using a low-conductivity electroporation medium enhanced the formation of both transient and irreversible membrane pores. We also found that clathrin-mediated endocytosis contributed to YO-PRO-1 uptake when a cell culture medium was used as an electroporation medium.

show abstract

Correlation between the loss of intracellular molecules and cell viability after cell electroporation

Cited by 29 publications

References 54 publications

The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation

The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation

Clinical Applications and Immunological Aspects of Electroporation-Based Therapies

Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers

Contact Info

Product

Resources

About