Correlation between type of alkali rinsing, cytotoxicity of bio-nanocellulose and presence of metabolites within cellulose membranes

Junka, Adam; Fijałkowski, Karol; Ząbek, Adam; Mikołajewicz, Katarzyna; Chodaczek, Grzegorz; Szymczyk, Patrycja; Smutnicka, Danuta; Żywicka, Anna; Sedghizadeh, Parish P.; Dziadas, Mariusz; Młynarz, Piotr; Bartoszewicz, Marzenna

doi:10.1016/j.carbpol.2016.10.007

Cited by 18 publications

(11 citation statements)

References 21 publications

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“…We also suggest that in a patient's oral cavity, BC dressings may efficiently absorb blood, exudate, and sequestrate microorganisms as we have shown in previous experiments [49]. In another work of ours, we confirmed the high biocompatibility of a BC dressing toward a fibroblast cell line [50]. These results are in line with data provided by Wiegand et al [51], who demonstrated the high biocompatibility of BC chemisorbed with povidone-iodine and octenidine toward a keratinocyte cell line.…”

Section: Discussionsupporting

confidence: 92%

“…BC displays all the beneficial features of hydrogels of natural origin: nontoxicity, biocompatibility, and physiochemical suitability [30]. As we have shown in our earlier work, BC does not induce macrophages to release free radicals [50]. The main potential disadvantage of a BC carrier may be related with certain level of differences in porosity and thickness, typical for natural biomaterials, which have an impact on water-related properties [53].…”

Section: Discussionmentioning

confidence: 87%

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Potential of Novel Bacterial Cellulose Dressings Chemisorbed with Antiseptics for the Treatment of Oral Biofilm Infections

Krasowski¹,

Wicher-Dudek

Paleczny

et al. 2019

Applied Sciences

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Infections of the oral cavity are caused by multicellular communities of microbes, referred to as biofilms. Due to the high tolerance of biofilms to antibiotics and specific conditions within the oral cavity, there is an ongoing search for carriers that are able to deliver high local concentrations of potent antimicrobials that can eradicate pathogenic biofilms. Bacterial cellulose, owing to its high flexibility, absorbance, and release potential, meets these demands. In this work we chemisorbed bacterial cellulose with antiseptics containing povidone-iodine or polihexanide and analyzed their ability to eradicate in vitro biofilms formed by oral pathogens, such as Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, Candida albicans, Streptococcus mutans, Staphylococcus aureus, and Pseudomonas aeruginosa. In tests performed by means of standard laboratory methods and with a long contact time (24 h), all antiseptics released from the cellulose dressings displayed a very high antibiofilm efficacy. On the other hand, when conditions imitating the oral cavity were used and cellulose dressings were applied for a 0.5–1 h contact time, the antiseptics released from the dressings displayed lower, though still acceptable, activity. Our findings indicate that besides species-specific resistance to particular antiseptic agents, environmental and experimental settings play an essential role in outcomes. Finally, in a proof-of-concept experiment performed in an oral cavity typodont model, we demonstrated the high flexibility and adhesiveness of antiseptic-containing cellulose dressings. Our novel findings, if developed in further studies, may lead to the introduction of new types of dressings that are able to efficiently deal with biofilm infections of the oral cavity.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 87%

Potential of Novel Bacterial Cellulose Dressings Chemisorbed with Antiseptics for the Treatment of Oral Biofilm Infections

Krasowski¹,

Wicher-Dudek

Paleczny

et al. 2019

Applied Sciences

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“…It also has macroparameters such as elasticity, and high mechanical strength, which is of particular importance in the case of bone loss . In spite of our positive results, we recognize the following limitations on this study: (1) culturing conditions; (2) variables in the production of BC, such as the initial bacterial inoculum, temperature range, medium composition, oxygen availability, and the choice of the culturing container shape or depth; and (3) BC cleaning and cells' removal may significantly affect such important BC parameters, such as cytotoxicity and metabolic content within disks . Being aware of these potential limitations of our study, we obtained all BC disks from one initial inoculum and removed K. xylinus cells during one cycle of NaOH treatment.…”

Section: Discussionmentioning

confidence: 89%

“…[14][15][16] In spite of our positive results, we recognize the following limitations on this study: (1) culturing conditions 17,18 ; (2) variables in the production of BC, such as the initial bacterial inoculum, temperature range, medium composition, oxygen availability, and the choice of the culturing container shape or depth 8,19,20 ; and (3) BC cleaning and cells' removal may significantly affect such important BC parameters, such as cytotoxicity and metabolic content within disks. 21 Being aware of these potential limitations of our study, we obtained all BC disks from one initial inoculum and removed K. xylinus cells during one cycle of NaOH treatment. Our approach resulted in repeatable outcomes and low cytotoxicity for osteoblast cells for BC alone ( Figure 5) and showed that despite the abovementioned challenges, BC is an extremely promising material for a carrier of antimicrobial suited against biofilmbased infections.…”

Section: Discussionmentioning

confidence: 99%

Application of bacterial cellulose experimental dressings saturated with gentamycin for management of bone biofilm in vitro and ex vivo

et al. 2019

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Bacterial cellulose is one of the most promising polymers of recent years. Herein, we present a possibility of BC application as a carrier of gentamycin antibiotic for the treatment and prevention of bone infections. We have shown that BC saturated with gentamycin significantly reduces the level of biofilm‐forming bone pathogens, namely Staphylococcus aureus and Pseudomonas aeruginosa, and displays very low cytotoxicity in vitro against osteoblast cell cultures. Another beneficial feature of our prototype dressing is prolonged release of gentamycin, which provides efficient protection from microbial contamination and subsequent infection. Moreover, it seems that bacterial cellulose (BC) alone without any antimicrobial added, may serve as a barrier by significantly hampering the ability of the pathogen to penetrate to the bone structure. Therefore, a gentamycin‐saturated BC dressing may be considered as a possible alternative for gentamycin collagen sponge broadly used in clinical setting. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:30–37, 2020.

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“…Cellulose produced by bacteria had a specific character and more purer than plant cellulose [1]. Plant cellulose is impure because of containing a complex carbohydrates like cellulose (35-50%), hemicellulose (20-35%) and lignin (10-25%) [2] [3]. Nata, known as bacterial cellulose (BC), able to be produced using fruit extracts [4] [5].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Chemical Pretreatment Process on Mechanical Properties and Porosity of Cellulose Bacterial Film

Sutrisno

Suryanto

Wulandari

et al. 2019

JMEST

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Bacterial cellulose (BC) is a natural polymer which have superior properties, like high porosity, high purity, and high permeability. The study objective is to determine the influence of chemical pretreatment on tensile strength and the porosity of BC. The method was to make BC films from pineapple peel extract through fermentation process for 14 days. The pretreatment was conducted by immersion of BC in BmimCl, H2O2, and NaOH solution with a concentration of 2.5%; 5%; and 7.5 %, heated at 80 °C then dried in the oven, and the samples were then tested by a tensile test using ASTM-D636-V standard, morphology analysis using Scanning Electron Microscope, and porosity analysis. The results indicate that the tensile strength of control sample was 123 MPa, whereas after chemical pretreatment, the tensile strength was decreased with the greater reduction occurred using NaOH pretreatment compared than the other solutions that having a lower tensile strength of 8.54 MPa at 7.5 % of NaOH. The results of porosity show that the value increased after being treated chemically. The BC film porosity was 87.13% after NaOH treatment of 7.5% while BC film untreated had porosity of 19.15%. This phenomenon was occurred due to the increasing pore, so the absorption of water increased.

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Correlation between type of alkali rinsing, cytotoxicity of bio-nanocellulose and presence of metabolites within cellulose membranes

Cited by 18 publications

References 21 publications

Potential of Novel Bacterial Cellulose Dressings Chemisorbed with Antiseptics for the Treatment of Oral Biofilm Infections

Potential of Novel Bacterial Cellulose Dressings Chemisorbed with Antiseptics for the Treatment of Oral Biofilm Infections

Application of bacterial cellulose experimental dressings saturated with gentamycin for management of bone biofilm in vitro and ex vivo

The Effect of Chemical Pretreatment Process on Mechanical Properties and Porosity of Cellulose Bacterial Film

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