Correlation Links Between Some Economically Valuable Signs in Broccoli

Zablotskaya, E. A.; Бондарева, Л. Л.; Sirota, S. M.

doi:10.18619/2072-9146-2018-1-8-11

Cited by 2 publications

(1 citation statement)

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“…The basic technology protocol for producing doubled haploids using isolated microspore culture in vitro, developed in the early 1980s [ 7 ] for rapeseed, was subsequently adapted by scientists for different members of the genus Brassica L. [ 8 , 9 , 10 ]. In the Russian Federation, this technique has been successfully applied to members of the Brassicaceae family, and F1 hybrids based on doubled haploid cabbage lines of white cabbage, Chinese cabbage, broccoli, and kohlrabi were obtained [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. As early studies show, the protocol is not standard and needs to be adapted to the specific species and genotype.…”

Section: Introductionmentioning

confidence: 99%

Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Mineykina

Бондарева

Солдатенко

et al. 2021

Plants

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Red cabbage belongs to the economically important group of vegetable crops of the Brassicaceae family. A unique feature of this vegetable crop that distinguishes it from other members of the family is its unique biochemical composition characterized by high anthocyanin content, which gives it antioxidant properties. The production mainly uses F1 hybrids, which require constant parental lines, requiring 6–7 generations of inbreeding. Culture of isolated microspores in vitro is currently one of the promising methods for the accelerated production of pure lines with 100% homozygosity. The aim of this study is to investigate the factors and select optimal parameters for successful induction of red cabbage embryogenesis in isolated microspore culture in vitro and subsequent regeneration of DH plants. As a result of research, for the first time, it was possible to carry out the full cycle of obtaining DH plants of red cabbage from the induction of embryogenesis to their inclusion in the breeding process. The size of buds containing predominantly microspores at the late vacuolated stage and pollen at the early bi-cellular stage has to be selected individually for each genotype, because the embryoid yield will be determined by the interaction of these two factors. In the six samples studied, the maximum embryoid yield was obtained from buds 4.1–4.4 mm and 4.5–5.0 mm long, depending on the genotype. Cultivation of microspores was carried out on liquid NLN culture medium with 13% sucrose. The maximum number of embryoids (173.5 ± 7.5 pcs./Petri dish) was obtained on culture medium with pH 5.8 and heat shock at 32 °C for 48 h. Successful embryoid development and plant regeneration by direct germination from shoot apical meristem were achieved on MS culture medium with 2% sucrose and 0.7% agar, supplemented with 6-benzylaminopurine at a concentration of 1 mg/L. Analysis of the obtained regenerated plants, which successfully passed the stage of adaptation to ex vitro conditions by flow cytometry, showed that most of them were doubled haploids (up to 90.9%). A low number of seeds produced by self-fertilization in DH plants was observed.

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Section: Introductionmentioning

confidence: 99%

Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Mineykina

Бондарева

Солдатенко

et al. 2021

Plants

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Variability of Characters of Winter Garlic Varieties Under the Conditions of Forest-Steppe of the Altai Regions' Ob River Area

2018

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The variability or stability of economically valuable features of varieties under cultivation conditions are very important indices. Environmental factors affect plants throughout the entire growing season. The research goal is to study the changes and stabilization of the characters that determine the phenotype of winter garlic when sampling from the variety accessions in the forest-steppe of the Altai Regions' Ob River Area. In the course of research from 2003 through 2007, the local forms and variety accessions from various regions of Russia were studied. The research was carried out according to the methodology guidelines. The variability of seven quantitative characters was investigated. In our study, the most stable characters were the "number of leaves" and "leaf length". The characters "number of cloves in a bulb" and "clove weight" are very difficult to stabilize. The variability of these indices was mainly medium and high. The characters with insignificant variability coefficient under the conditions of the south of West Siberia were revealed. The percentage of the factors' influence on the variability of the "bulb weight" character was determined. The following most stable variety accessions for the conditions of the forest-steppe of the Altai Regions' Ob River Area were identified: the varieties Skif, Kasmala and German, and the local forms K 3 and K 56.

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Correlation Links Between Some Economically Valuable Signs in Broccoli

Cited by 2 publications

References 1 publication

Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Variability of Characters of Winter Garlic Varieties Under the Conditions of Forest-Steppe of the Altai Regions' Ob River Area

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