Correlation of Goblet Cell Density and Mucosal Epithelial Membrane Mucin Expression with Rose Bengal Staining in Patients with Ocular Irritation

Pflugfelder, Stephen C.; Tseng, Scheffer C.G.; Yoshino, Ken‐ichi; Monroy, Dagoberto; Félix, Carlos Roberto; Reis, Brenda L.

doi:10.1016/s0161-6420(97)30330-3

Cited by 208 publications

(136 citation statements)

References 25 publications

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“…This is in contrast to the findings of Pflugfelder et al, 8 who observed a correlation of goblet cell density, and an inverse correlation between the degree of squamous metaplasia with Schirmer I test results in patients with ocular irritation.…”

Section: Discussioncontrasting

confidence: 99%

“…Our results are in concordance with Lee and co-workers, 25 who found that the severity of squamous metaplasia correlated well with the intensity of the rose bengal score, and with Pflugfelder et al, 8 who described a correlation between goblet cell density and rose bengal staining. Bjerrum 26 found a correlation between nuclear changes and rose bengal staining, which was also seen in our population.…”

Section: Discussionsupporting

confidence: 93%

“…Pathological changes, such as squamous metaplasia of the epithelial cells, keratinization, and reduction of goblet cell density up to total goblet cell loss, occur in numerous disorders such as dry eye, Sjö gren's syndrome, blepharokonjunctivitis, inflammatory keratokonjunctivitis, and vitamin A deficiency. [3][4][5][6][7][8][9][10][11][12] For staging of the morphological changes of the conjunctiva, several grading systems have been developed. The first grading system published by Nelson et al 3 was based on the morphological appearance of the conjunctival epithelial and goblet cells, which was completed in 1988 4 by the so-called mean individual epithelial cell area, according to the degree of squamous metaplasia, and the number of goblet cells.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating an impression cytology grading system (IC score) in patients with dry eye syndrome

Schwantzer

Berghold

et al. 2005

Eye

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Purpose To evaluate the IC score, a newly developed grading system for the morphological alterations of the ocular surface as a diagnostic tool in relation to type and severity of dry eye disease. Methods Impression cytological specimens of 309 patients with simple dry eye (S-DE), 50 patients with Sjö gren's syndrome and dry eye (SS-DE), and 39 patients with connective tissue disease and dry eye (CTD-DE) were examined. In all, 10 different morphological parameters were judged with points (IC subscores) and summarized to the IC score. IC score and subscores were correlated to tear function test results and with each other. Results SS-DE patients had the highest IC scores and IC subscores. IC scores and IC subscores correlated significantly with the fluorescein staining of the cornea in all three patient groups. They were correlated to the rose bengal score in SS-DE and S-DE patients. The analysis of the IC subscores showed that there are significant correlations between most of the morphological alterations investigated. This underlines the importance of each parameter for the summarized IC score. Conclusion The IC score provides detailed information about the morphological alterations of the bulbar conjunctiva. It has proven to be useful for the differentiation of dry eye diseases and might help in the investigation of the pathogenesis of these diseases as well as for follow-up and therapy control.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Evaluating an impression cytology grading system (IC score) in patients with dry eye syndrome

Schwantzer

Berghold

et al. 2005

Eye

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“…Danjo & Hamano (1995) described abnormal non-contact specular microscopy of the lipid layer in patients with Sjögren's syndrome and Shimazaki et al (1998) showed destruction of the Meibomian glands in a large proportion of patients. There are also recent reports of reduced goblet cell density and reduced expression of epithelial membrane mucin in patients with Sjögren's syndrome (Pflugfelder et al, 1997;Jones et al, 1998). Our finding of reduced surface activity of tear fluid is compatible with reduced function of the Meibomian glands and/or reduced mucin production and thus supports the notion of involvement of these glands in the disease process.…”

Section: Discussionsupporting

confidence: 87%

“…Although it is well established that there are major differences in the water layer between normal tear fluid and tear fluid in keratoconjunctivitis sicca (Friedlaender, 1992), it has recently been suggested that there are also abnormalities of the lipid layer of the tear film (Danjo & Hamano, 1995;Shimazaki et al, 1998) as well as in mucin production (Pflugfelder et al, 1997;Jones et al, 1998) in Sjögren's syndrome.…”

Section: Introductionmentioning

confidence: 99%

Surface activity of tear fluid in patients with primary Sjogren's syndrome

Jiang¹,

Manthorpe²,

Wollmer³

2002

Clin Physiol

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Goblet Cell Numbers and Epithelial Proliferation in the Conjunctiva of Patients With Dry Eye Syndrome Treated With Cyclosporine

Kunert

Tisdale²,

Gipson³

2002

Arch Ophthalmol

318

201

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To compare conjunctival goblet cell numbers as well as epithelial turnover in patients with non-Sjö gren syndrome-associated keratoconjunctivitis sicca (NSS-KCS) and those with SS-KCS before and after 6 months of treatment with topical cyclosporine A (CsA) ophthalmic emulsion.Methods: Conjunctival biopsy specimens from 16 patients with NSS-KCS and 12 with SS-KCS were obtained at baseline and after 6 months' therapy with CsA or vehicle alone. Conjunctival biopsy specimens were also obtained from 11 normal subjects. Periodic acid-Schiff staining determined the number of goblet cells present. Immunofluorescence microscopy for Ki-67 localization was used to evaluate the number of actively cycling cells.Results: Periodic acid-Schiff staining showed fewer goblet cells at baseline in both dry eye populations when compared with normal subjects (PϽ.001). After 6 months of CsA treatment, conjunctival biopsy specimens of both NSS-KCS and SS-KCS groups revealed an increase in goblet cells compared with baseline (PϽ.05). More Ki-67positive cells were observed in NSS-KCS conjunctiva at baseline than in normal conjunctiva (PϽ.05) whereas numbers of these cells in SS-KCS conjunctiva were similar to normal at baseline. After 6 months of CsA treatment, conjunctival biopsy specimens of NSS-KCS revealed a decrease in Ki-67-labeled cells compared with baseline (PϽ.001). In contrast, no substantial change was observed for CsA treatment in patients with SS-KCS.Conclusions: Treatment of dry eye syndrome for 6 months with topical CsA resulted in an increase in goblet cell numbers in patients with NSS-KCS and SS-KCS and a decrease in epithelial turnover in those with NSS-KCS. Reducing ocular surface inflammation might have an effect on the proliferative activity of the epithelium.

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Correlation of Goblet Cell Density and Mucosal Epithelial Membrane Mucin Expression with Rose Bengal Staining in Patients with Ocular Irritation

Cited by 208 publications

References 25 publications

Evaluating an impression cytology grading system (IC score) in patients with dry eye syndrome

Evaluating an impression cytology grading system (IC score) in patients with dry eye syndrome

Surface activity of tear fluid in patients with primary Sjogren's syndrome

Goblet Cell Numbers and Epithelial Proliferation in the Conjunctiva of Patients With Dry Eye Syndrome Treated With Cyclosporine

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