Correlation of Histopathological Variants, Cellular DNA Content, and Clinical Outcome in Adenoid Cystic Carcinoma of the Salivary Glands

Abstract

B-3.

DNA Aneuploidy in Malignant Salivary Gland Neoplasms is Independent of USP44 Protein Expression

“…In addition to the above mentioned, tumor heterogeneity may also account for the lack of association of USP44 expression with ploidy, as different group of cells were used in each assay. Tumor cell DNA ploidy has been linked to behavior of different types of neoplasms, including salivary gland tumors (7,14,15). In agreement to reported by others (7,15), in this subset of investigated malignant tumors was identified varying DNA content among different tumor types, with high percentage of PLGA cases (71,5%) and all ACCs were diploid (8).…”

DNA ploidy analysis in salivary gland tumours by image cytometry

“…A vast majority of studies have used FCM to evaluate DNA content in salivary gland tumours, and the samples have been obtained from fresh tumour tissue or paraffin blocks (10–13, 18, 22–34). Takashima et al (13) suggested that a combination of magnetic resonance, cytology and FCM is optimal for diagnosing malignancies of parotid gland, and fine needle aspiration biopsy (FNAB) derived materials can replace the surgical specimens in FCM analysis.…”

“…There are few large series published in the English language focusing on ploidy analysis for the prediction of tumour aggressiveness, prognosis, or to distinguish benign and malignant salivary gland tumours (12, 17, 18, 23, 28). Some authors reported that DNA diploidy may be seen in both benign and malignant lesions, but aneuploidy is mainly seen in malignant lesions (12, 24).…”

“…Three of the four recent large series published by Driemel et al (18) ( n = 279), Pinto et al (12) ( n = 97) and Enamorado et al . (23) ( n = 46) have studied the ploidy status on fresh samples of salivary gland tumours by FCM. These authors have worked with different diagnostic criteria (CV, DI, histogram peaks and minimum number of tumour cell‐nuclei) to interpret the histograms as diploid or aneuploid.…”

Adenoid cystic carcinomas of the breast and salivary glands (or ‘The strange case of Dr Jekyll and Mr Hyde’ of exocrine gland carcinomas)

“…45 46 The presence of aneuploidy appears to be less common in the cribriform variant (16%) than in the solid lesions (67%, table 3). 53 Interestingly, a TP53 point mutation and p53 expression have been demonstrated in the dedifferentiated component of two dedifferentiated AdCCs, together with HER2 overexpression and a high proliferation index 43…”