Correlation of inflammation parameters and biochemical markers of cholestasis with the intensity of lipid peroxidation in patients with choledocholithiasis

Damnjanović, Zoran; Jovanovic, Milan; Nagorni, Aleksandar; Radojković, Milan; Sokolović, Dušan; Damnjanović, Goran; Djindjić, Boris; Smiljkovic, Igor; Kamenov, Aleksandar; Damnjanović, Ivana

doi:10.2298/vsp1302170d

Cited by 18 publications

(13 citation statements)

References 37 publications

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“…Cholestasis appears when the bile drainage to intestine is impaired due to benign or malignant conditions. This causes bile regurgitation to the blood with the consequent retention of bile constituents in the liver [17]. After cessation of bile flow, dilatation of extrahepatic and intrahepatic bile ducts and alteration of portal tracts occur in a few days.…”

Section: Discussionmentioning

confidence: 99%

Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats

Solmaz

Gülçiçek

Erçetin

et al. 2016

Bosn J of Basic Med Sci

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Obstructive jaundice (OJ) can be defined as cessation of bile flow into the small intestine due to benign or malignant changes. Nesfatin-1, recently discovered anorexigenic peptide derived from nucleobindin-2 in hypothalamic nuclei, was shown to have anti-inflammatory and antiapoptotic effects. This study is aimed to investigate the therapeutic effects of nesfatin-1 on OJ in rats. Twenty-four adult male Wistar-Hannover rats were randomly assigned to three groups: sham (n = 8), control (n = 8), and nesfatin (n = 8). After bile duct ligation, the study groups were treated with saline or nesfatin-1, for 10 days. Afterward, blood and liver tissue samples were obtained for biochemical analyses, measurement of cytokines, determination of the oxidative DNA damage, DNA fragmentation, and histopathologic analyses. Alanine aminotransferase and gamma-glutamyl transferase levels were decreased after the nesfatin treatment; however, these drops were statistically non-significant compared to control group (p = 0.345, p = 0.114). Malondialdehyde levels decreased significantly in nesfatin group compared to control group (p = 0.032). Decreases in interleukin-6 and tumor necrosis factor-α levels from the liver tissue samples were not statistically significant in nesfatin group compared to control group. The level of oxidative DNA damage was lower in nesfatin group, however this result was not statistically significant (p = 0.75). DNA fragmentation results of all groups were similar. Histopathological examination revealed that there was less neutrophil infiltration, edema, bile duct proliferation, hepatocyte necrosis, basement membrane damage, and parenchymal necrosis in nesfatin compared to control group. The nesfatin-1 treatment could alleviate cholestatic liver damage caused by OJ due to its anti-inflammatory and antioxidant effects.

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Section: Discussionmentioning

confidence: 99%

Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats

Solmaz

Gülçiçek

Erçetin

et al. 2016

Bosn J of Basic Med Sci

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“…In BDL model, the hepatocellular excretion of bile constituents is markedly impaired eliciting its retention within hepatocytes. Cholestasis syndrome includes liver function disorder attributable to the obstruction of bile drainage into the intestine, with the consequent retention of bile constituents in the liver and their regurgitation in the blood [ 54 , 55 ]. In the condition of cholestasis, primarily hydrophobic bile salts and bilirubin have the most significant toxic effects [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats

Fahmy

2015

BMC Complement Altern Med

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BackgroundHolothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats.MethodsFifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight).ResultsThe HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities.ConclusionThe present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid.

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“…Furthermore, oxidized PLs (oxPLs) are abundantly found at sites of inflammation and are considered to play an active role in the modulation of the immune response [36]. The relationship between active oxygen species, lipid peroxidation and necrosis has been highly demonstrated in many pathological processes [37–39]. …”

Section: Resultsmentioning

confidence: 99%

Towards Lipidomics of Low-Abundant Species for Exploring Tumor Heterogeneity Guided by High-Resolution Mass Spectrometry Imaging

Cimino

Calligaris

Far

et al. 2013

IJMS

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Many studies have evidenced the main role of lipids in physiological and also pathological processes such as cancer, diabetes or neurodegenerative diseases. The identification and the in situ localization of specific low-abundant lipid species involved in cancer biology are still challenging for both fundamental studies and lipid marker discovery. In this paper, we report the identification and the localization of specific isobaric minor phospholipids in human breast cancer xenografts by FTICR MALDI imaging supported by histochemistry. These potential candidates can be further confirmed by liquid chromatography coupled with electrospray mass spectrometry (LC-ESI-MS) after extraction from the region of interest defined by MALDI imaging. Finally, this study highlights the importance of characterizing the heterogeneous distribution of low-abundant lipid species, relevant in complex histological samples for biological purposes.

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Correlation of inflammation parameters and biochemical markers of cholestasis with the intensity of lipid peroxidation in patients with choledocholithiasis

Cited by 18 publications

References 37 publications

Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats

Nesfatin-1 alleviates extrahepatic cholestatic damage of liver in rats

Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats

Towards Lipidomics of Low-Abundant Species for Exploring Tumor Heterogeneity Guided by High-Resolution Mass Spectrometry Imaging

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