Correlation of polypeptide composition with antigenic variation in the swine vesicular disease and coxsackie B5 viruses

Harris, T. J. R.; Brown, F.

doi:10.1038/258758a0

Cited by 34 publications

(13 citation statements)

References 16 publications

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“…SVDV has been found to be closely related to CB5 in its physicochemical, biochemical and serological characteristics, while immunodiffusion, neutralization and RNA hybridization have shown differences between them (Harris et al, 1977). CB5 shows considerable variation (Brown & Wild, 1974), but the variation of SVDV is smaller (Harris & Brown, 1975). Graves (1973) suggested that SVDV was originally derived from human CB5.…”

Section: The 3' Non-coding Regionmentioning

confidence: 99%

The Complete Nucleotide Sequence of Swine Vesicular Disease Virus

Inoue

Suzuki

Sekiguchi

1989

Journal of General Virology

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SUMMARYThe complete nucleotide sequence of the genome of the enterovirus swine vesicular disease virus (SVDV ; H/3 '76) isolated from a healthy pig has been determined using molecular cloning and DNA sequencing techniques. The RNA genome was 7400 nucleotides long, excluding the poly(A) tract, and appeared to encode a single polyprotein of 2185 amino acids. The predicted amino acid sequence of the polyprotein showed close homology (around 90 ~) to that of the previously sequenced coxsackieviruses B1, B3 and B4, and also showed homology (around 60~) to that of poliovirus. This homology allows us to predict the possible cleavage sites of the polyprotein and to identify other features of structural and functional significance, which seem to be important to the biological integrity of the virus. A detailed analysis of homology between SVDV and coxsackieviruses shows that non-structural proteins are highly conserved whereas the structural proteins are less well conserved. The 5' and 3' noncoding regions are also conserved, although there are several divergent nuclcotide stretches. These stretches may differentiate SVDV from coxsackieviruses.

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Section: The 3' Non-coding Regionmentioning

confidence: 99%

The Complete Nucleotide Sequence of Swine Vesicular Disease Virus

Inoue

Suzuki

Sekiguchi

1989

Journal of General Virology

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“…Changes in the polypeptide composition of more recent coxsackievirus B5 isolates, when compared to the prototype virus isolated in 1952, are associated with the RNA genomic changes described above. In 1975, however, a swine virus and a human isolate of coxsackievirus B5 showed virtually identical patterns (31).…”

Section: Properties Of the Enterovirusesmentioning

confidence: 99%

My Role in the Discovery and Classification of the Enteroviruses

Melnick

1996

Annu. Rev. Microbiol.

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The enteroviruses constitute one of the genera of the picornavirus family. The genus includes the polioviruses, the coxsackieviruses, and the echoviruses of humans, plus a number of enteroviruses of lower animals (e.g. monkeys, cattle, pigs, mice). Over 100 serotypes are recognized, of which the first to be discovered were the polioviruses. It was my good fortune to have been a scientist during the golden age of virology, when new techniques were being introduced into the field. These often led to the discovery of new viruses. This article details the isolation of the enteroviruses, their recognition as a separate genus of Picornaviridae, and my role in the process. Poliovirus, the most hazardous of the group, is almost gone from the world, but the other enteroviruses will be with us for some time. Several members of the Committee dealing with these agents-Enders, Sabin, Dalldorf, Syverton-have passed on, but the work of this Committee to which I was privileged to contribute will live long. CONTENTS

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“…However, hyperimmune guinea pig sera can be used to differentiate between SVDV isolates in immunodiffusion tests with purified viruses. These differences appeared to correlate with altered migration of the virus particle polypeptides in polyacrylamide gels (9).…”

mentioning

confidence: 92%

“…For the growth of radioactively labeled virus, cells were infected at high multiplicity with virus grown in IBRS-2 cells and incubated in Eagle medium lacking methionine but containing 1 to 5 tCi of [35S]methionine per ml (9). heating at 100°C for 2 min, the mixture was analyzed on high-resolution polyacrylamide slab gels using the discontinuous buffer system of Laemmli (11) as described previously (9). The main gel (12.5%) and the stacking gel (5%), containing 0.5 M urea, were subjected to electrophoresis for 3 to 4 h at 100 V. The gels were stained with 0.25% Coomassie blue in methanolacetic acid-water (2:1:7), destained in methanol-acetic acid-water, dried under vacuum onto wettable cellophane, and autoradiographed with Kodirex X-ray film.…”

mentioning

confidence: 99%

Molecular approach to the epidemiology of swine vesicular disease: correlation of variation in the virus structural polypeptides with serological properties

Harris¹,

Underwood²,

Knowles³

et al. 1979

Infect Immun

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Purification of viruses. The method was described in detail previously (10).PAGE. The sucrose gradient peaks of [;DS]methionine-labeled purified virus were diluted with 0.1 M NaCl-0.05 M tris(hydroxymethyl)aminomethane-hydrochloride (pH 7.6) containing 50 to 75,tg of bovine plasma albumin, and the virus was precipitated with 2 volumes of acetone at -20'C overnight. The precipitate was collected by centrifugation, drained well, and dissolved in 50 to 100 !l of disruption buffer [0.0625 M tris(hydroxymethyl)aminomethane-hydrochloride, pH 6.8, containing 0.5 M urea, 2% sodium dodecyl sulfate, 2% mercaptoethanol, and 10% glycerol]. After 593 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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Correlation of polypeptide composition with antigenic variation in the swine vesicular disease and coxsackie B5 viruses

Cited by 34 publications

References 16 publications

The Complete Nucleotide Sequence of Swine Vesicular Disease Virus

The Complete Nucleotide Sequence of Swine Vesicular Disease Virus

My Role in the Discovery and Classification of the Enteroviruses

Molecular approach to the epidemiology of swine vesicular disease: correlation of variation in the virus structural polypeptides with serological properties

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