Correlation of scanning electron microscope and light microscope images of individual cells in human blood and blood clots

McDonald, Larry W.; Hayes, T.

doi:10.1016/0014-4800(69)90039-2

Cited by 28 publications

(18 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, light microscope and conventional electron microscope facilities provide information which makes the scanning electron micrograph very much more useful (KOEHLER and HAYES, 1969a;KOEHLER and HAYES, 1969b;McDoNALD and HAYES, 1969;McALLISTER and HADEK, 1970). An SEM isolated from these facilities, particularly in biological research, would be difficult to use to its full potential.…”

Section: What Auxilliary Equipment Might Be Needed?mentioning

confidence: 96%

“…Occasionally however, this surface tension flattening can be an advantage (e.g. in the disclosure of internal granules of a cell which would not be revealed if the spherical form of the cell were maintained through freeze drying or critical point drying, McDoNALD and HAYES, 1969). Cell junctions may be emphasized by air drying (SPENCER and HAYES, 1970), and the A, I, and Z bands in striated muscle fibers can also be accentuated by this technique.…”

Section: Air Dryingmentioning

confidence: 97%

See 1 more Smart Citation

Scanning Electron Microscope Techniques in Biology

Hayes¹

1973

Advanced Techniques in Biological Electron Microscopy

Self Cite

View full text Add to dashboard Cite

Section: What Auxilliary Equipment Might Be Needed?mentioning

confidence: 96%

Section: Air Dryingmentioning

confidence: 97%

Scanning Electron Microscope Techniques in Biology

Hayes¹

1973

Advanced Techniques in Biological Electron Microscopy

Self Cite

View full text Add to dashboard Cite

“…It is often helpful in interpreting a scanning electron microscope (SEM)-image to be able to re-examine precisely the same area with the light microscope (LM) (McDonald & Hayes, 1969;Geissinger & Bond, 1971). Conversely LM-images can be further defined with the higher axial and lateral resolution of the SEM (McDonald, Pease & Hayes, 1967;Geissinger, 1971).…”

Section: Introductionmentioning

confidence: 99%

A precise stage arrangement for correlative microscopy for specimens mounted on glass slides, stubs or EM grids

Geissinger

1974

Journal of Microscopy

View full text Add to dashboard Cite

Summary The instrumentation necessary for precise and fast correlation of images derived from a light optical microscope (LM) and a scanning electron microscope (SEM) operated in the reflective mode, is described. The specimens can be mounted on standard microscope slides (25 × 75 mm), SEM‐stubs (12 mm ø), or on transmission EM grids (3 mm ø). The instrumentation consists of two parts: an attachable precision stage for an LM, and an attachable slide carrier for the stage of an SEM. By taking into account the vernier readings of the stages of both microscopes (LM and SEM), identical particles in a specimen can be found instantaneously under either microscope. Therefore it is concluded that the use of this instrumentation in correlative microscopy (LM → SEM → LM) is time saving, and especially recommended on fragile biological specimens, which may deteriorate rapidly under the electron beam of an SEM.

show abstract

“…The first paper to coin the phrase 'correlative microscopy' appeared in 1969 (McDonald and Hayes 1969), but it was Geissinger (Geissinger 1974) who, in a brief technical note five years later, elegantly summarized the challenges that must be met when one seeks to capture exactly the same structures on the same microscopic sample by light microscopy (LM) and EM. The first challenge is adapting and fine-tuning the sample preparation to ensure suitability for both imaging modes; the second is guaranteeing that the predetermined area of interest will be examined under both modes; the third, which is intimately linked to the second, is the development of precise and exchangeable sample supports and/or stage facilities that are interchangeable between the different microscope platforms.…”

mentioning

confidence: 99%

Multi-dimensional correlative imaging of subcellular events: combining the strengths of light and electron microscopy

Nykanen

Jahn

et al. 2010

Biophys Rev

View full text Add to dashboard Cite

To genuinely understand how complex biological structures function, we must integrate knowledge of their dynamic behavior and of their molecular machinery. The combined use of light or laser microscopy and electron microscopy has become increasingly important to our understanding of the structure and function of cells and tissues at the molecular level. Such a combination of two or more different microscopy techniques, preferably with different spatial-and temporal-resolution limits, is often referred to as 'correlative microscopy'. Correlative imaging allows researchers to gain additional novel structurefunction information, and such information provides a greater degree of confidence about the structures of interest because observations from one method can be compared to those from the other method(s). This is the strength of correlative (or 'combined') microscopy, especially when it is combined with combinatorial or non-combinatorial labeling approaches. In this topical review, we provide a brief historical perspective of correlative microscopy and an in-depth overview of correlative sample-preparation and imaging methods presently available, including future perspectives on the trend towards integrative microscopy and microanalysis.

show abstract

Correlation of scanning electron microscope and light microscope images of individual cells in human blood and blood clots

Cited by 28 publications

References 3 publications

Scanning Electron Microscope Techniques in Biology

Scanning Electron Microscope Techniques in Biology

A precise stage arrangement for correlative microscopy for specimens mounted on glass slides, stubs or EM grids

Multi-dimensional correlative imaging of subcellular events: combining the strengths of light and electron microscopy

Contact Info

Product

Resources

About