Correlation of viral RNA biosynthesis with glucose-6-phosphate dehydrogenase activity and host resistance

Šindelář, L.; Šindelářová, M.

doi:10.1007/s00425-002-0800-y

Cited by 25 publications

(3 citation statements)

References 31 publications

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“…G6PDH has been shown to respond to various oxidative stresses and viral infection at the level of mRNA abundance or enzyme activity (Batz et al 1998, Nemoto and Sasakuma 2000, Šindelář and Šindelářová 2002. To clarify whether the change of the G6PDH activity under heat stress results from elevated protein content or specific activity, Western-blot analysis was carried out.…”

Section: Resultsmentioning

confidence: 99%

“…It has been indicated that G6PDH plays a protective role against oxidative stress (Kletzien et al 1994, Pandolfi et al 1995. G6PDH is also involved in nitrogen assimilation (Oji et al 1985, Bowsher et al 1989, Esposito et al 2003, response to pathogenesis (Šindelář andŠindelářová 2002, Scharte et al 2009), metal toxicity (Esposito et al 1998), salt stress (Nemoto and Sasakuma, 2000, Liu et al 2007, Wang et al 2008 and combination of drought and heat stress (Rizhsky et al 2004). It was found that under salt stress G6PDH is involved in maintenance of intracellular reduced glutathione (GSH) in reed callus (Wang et al 2008) or NADPH content in olive plants (Valderrama et al 2006).…”

Section: Introductionmentioning

confidence: 96%

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Involvement of G6PDH in heat stress tolerance in the calli from Przewalskia tangutica and Nicotiana tabacum

Gong¹,

Chen²,

Li³

et al. 2012

Biologia plant.

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Glucose-6-phosphate dehydrogenase (G6PDH) has been implicated in supplying reduced nicotine amide cofactors for biochemical reactions and in modulating the redox state of cells. In this study, the role of G6PDH in thermotolerance of the calli from Przewalskia tangutica and tobacco (Nicotiana tabacum L.) was investigated. Results showed that Przewalskia tangutica callus was more sensitive to heat stress than tobacco callus. The activity of G6PDH and antioxidant enzymes (ascorbate peroxidase, catalase, peroxidase and superoxide dismutase) in calli from Przewalskia tangutica and tobacco increased after 40 °C treatment, although two calli exhibited a difference in the degree and timing of response to heat stress. When G6PDH was partially inhibited by glucosamine pretreatment, the antioxidant enzyme activities and thermotolerance in both calli significantly decreased. Simultaneously, the heat-induced H 2 O 2 content and the plasma membrane NADPH oxidase activity were also reduced. Application of H 2 O 2 increased the activity of G6PDH and antioxidant enzymes in both calli. Diphenylene iodonium, a NADPH oxidase inhibitor, counteracted heatinduced H 2 O 2 accumulation and reduced the heat-induced activity of G6PDH and antioxidant enzymes. Moreover, exogenous H 2 O 2 was effective in restoring the activity of G6PDH and antioxidant enzymes after glucosamine pretreatment. Western blot analysis showed that G6PDH gene expression in both calli was also stimulated by heat and H 2 O 2 , and blocked by DPI and glucosamine under heat stress. Taken together, under heat stress G6PDH promoted H 2 O 2 accumulation via NADPH oxidase and the elevated H 2 O 2 was involved in regulating the activity of antioxidant enzymes, which in turn facilitate to maintain the steady-state H 2 O 2 level and protect plants from the oxidative damage.Additional key words: antioxidant enzymes, hydrogen peroxide, tobacco.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Involvement of G6PDH in heat stress tolerance in the calli from Przewalskia tangutica and Nicotiana tabacum

Gong¹,

Chen²,

Li³

et al. 2012

Biologia plant.

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show abstract

“…In plants, G6PD activity is present in the plastids and cytosol (Debnam and Emes, 1999). G6PD activity has been positively correlated with environmental stresses such as salt stress (Valderrama et al, 2006;Wang et al, 2008), aluminum toxicity (Slaski et al, 2006), drought (Scharte et al, 2009), and infection by viral and fungal pathogens (Sindelár and Sindelárová, 2002;Scharte et al, 2009); by contrast, a decrease in chloroplastic G6PD activity has been shown to be beneficial for withstanding oxidative stress (Debnam et al, 2004). Thus, the role of G6PD in the stress response is currently unclear.…”

Section: Introductionmentioning

confidence: 99%

Stress-Induced GSK3 Regulates the Redox Stress Response by Phosphorylating Glucose-6-Phosphate Dehydrogenase in Arabidopsis

et al. 2012

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Diverse stresses such as high salt conditions cause an increase in reactive oxygen species (ROS), necessitating a redox stress response. However, little is known about the signaling pathways that regulate the antioxidant system to counteract oxidative stress. Here, we show that a Glycogen Synthase Kinase3 from Arabidopsis thaliana (ASKa) regulates stress tolerance by activating Glc-6-phosphate dehydrogenase (G6PD), which is essential for maintaining the cellular redox balance. Loss of stress-activated ASKa leads to reduced G6PD activity, elevated levels of ROS, and enhanced sensitivity to salt stress. Conversely, plants overexpressing ASKa have increased G6PD activity and low levels of ROS in response to stress and are more tolerant to salt stress. ASKa stimulates the activity of a specific cytosolic G6PD isoform by phosphorylating the evolutionarily conserved Thr-467, which is implicated in cosubstrate binding. Our results reveal a novel mechanism of G6PD adaptive regulation that is critical for the cellular stress response.

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Glucose-6-phosphate dehydrogenase plays a pivotal role in tolerance to drought stress in soybean roots

Liu

Wang

et al. 2012

Plant Cell Rep

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KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ) regulated GR, DHAR, and MDHAR activities to maintain GSH and Asc levels. Glucose-6-phosphate dehydrogenase (G6PDH) plays a pivotal role in plant resistance to environmental stresses. In this study, we investigated the role of G6PDH in modulating redox homeostasis under drought stress induced by polyethylene glycol 6000 (PEG6000) in two soybean cultivars JINDOU21 (JD-21) and WDD00172 (WDD-172). The G6PDH activity markedly increased and reached a maximum at 96 h in JD-21 and 72 h in WDD-172 during PEG6000 treatments, respectively. Glucosamine (Glucm, a G6PDH inhibitor) obviously inhibited G6PDH activity in both soybeans under PEG6000 treatments. After PEG6000 treatment, JD-21 showed higher tolerance than WDD-172 not only in higher activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR), but also in higher content of glutathione (GSH) and ascorbate (Asc). And we found that hydrogen peroxide (H(2)O(2)) regulated the cell length in root elongation zone. Diphenylene iodonium (DPI, a plasma membrane NADPH oxidase inhibitor) counteracted the PEG6000-induced H(2)O(2) accumulation and decreased the activities of GR, DHAR, and MDHAR as well as GSH and Asc content. Furthermore, exogenous application of H(2)O(2) increased the GR, DHAR, and MDHAR activities that were decreased by Glucm under drought stress. Western blot analysis showed that the G6PDH expression was stimulated by PEG6000 and buthionine sulfoximine (BSO, glutathione biosynthesis inhibitor), and blocked by Glucm, DPI and N-acetyl-L-cysteine (NAC, GSH precursor) in both cultivars. Taken together, our evidence indicates that G6PDH plays a central role in the process of H(2)O(2) regulated GR, DHAR, and MDHAR activities to maintain GSH and Asc levels.

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Correlation of viral RNA biosynthesis with glucose-6-phosphate dehydrogenase activity and host resistance

Cited by 25 publications

References 31 publications

Involvement of G6PDH in heat stress tolerance in the calli from Przewalskia tangutica and Nicotiana tabacum

Involvement of G6PDH in heat stress tolerance in the calli from Przewalskia tangutica and Nicotiana tabacum

Stress-Induced GSK3 Regulates the Redox Stress Response by Phosphorylating Glucose-6-Phosphate Dehydrogenase in Arabidopsis

Glucose-6-phosphate dehydrogenase plays a pivotal role in tolerance to drought stress in soybean roots

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