Correlations between the iodine concentrations from dual energy computed tomography and molecular markers Ki-67 and HIF-1α in rectal cancer: A preliminary study

Fan, Shuxuan; Li, Xubin; Zheng, Lei; Hu, Dongzhi; Ren, Xiaoyi; Ye, Zhaoxiang

doi:10.1016/j.ejrad.2017.08.026

Cited by 22 publications

(20 citation statements)

References 29 publications

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“…For evaluation of HIF-1 α expression, the number of nonpositive cells or positive cells was less than 1%, of which 1–10% of positive cells had low expression (+), 11–50% had medium expression (++), and more than 50% had high expression (+++). A staining result of 0 or 1+ was considered as negative and ≥2+ as positive [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Expression of GLUT3 and HIF-1α in Meningiomas of Various Grades Correlated with Peritumoral Brain Edema

Mei

Wang

et al. 2020

BioMed Research International

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Aim. To investigate the expression of glucose transporter 3 (GLUT3) and hypoxia-inducible factor-1α protein (HIF-1α) in meningiomas and analyze the correlation between GLUT3 and HIF-1α expression with the pathological grade of peritumoral brain edema (PTBE) of meningiomas. Methods. In this cross-sectional study, we analyzed meningioma specimens from 160 patients collected from January 1, 2014, to December 1, 2017, by dividing them into a low-grade (WHO I) or high-grade (WHO II and WHO III) group. Immunohistochemical analyses were used to detect the expression level of GLUT3 and HIF-1α in the tumor specimens. Results. The proportion of GLUT3-positive staining in tumors sized <4 cm, 4–6 cm, and>6 cm was 35.9% (37/103), 63.6% (28/44), and 53.8% (7/13), respectively (P=0.007). The proportion of HIF-1α-positive staining in tumors sized <4 cm, 4–6 cm, and >6 cm was 41.7% (43/103), 68.2% (30/44), and 38.5% (5/13), respectively (P=0.010). The proportion of GLUT3-positive staining in the high-grade group and low-grade group was 70.8% (34/48) and 33.9% (38/112), respectively (P<0.001). The proportion of HIF-1α-positive staining in the high-grade group and low-grade group was 62.5% (30/48) and 42.9% (48/112), respectively (P=0.023). GLUT3-positive expression in meningioma PTBE grades 0, I, II, and III was 20.3% (13/64), 41.2% (14/34), 63.6% (21/33), and 82.8% (24/29), respectively (Bonferroni-corrected, P<0.001, α/6=0.008). HIF-1α-positive expression in meningioma PTBE grades 0, I, II, and III was 34.4% (22/64), 47.1% (16/34), 54.5% (18/33), and 75.9% (22/29), respectively (Bonferroni-corrected, P=0.003, α/6=0.008). Spearman’s correlation analysis revealed a correlation between the expression of GLUT3 and HIF-1α in meningiomas (r=0.463, P<0.001). Multivariate analysis revealed that GLUT3-positive expression, HIF-1α-positive expression, and high pathological grade were associated with the development of PTBE (P<0.05). Conclusions. GLUT3 and HIF-1α expression in meningiomas was closely related to the tumor size, pathological grade, and PTBE. This study is the first to report a unique map-like multifocal GLUT3 staining pattern in meningiomas.

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Section: Methodsmentioning

confidence: 99%

Expression of GLUT3 and HIF-1α in Meningiomas of Various Grades Correlated with Peritumoral Brain Edema

Mei

Wang

et al. 2020

BioMed Research International

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“…Both the NIC and nZ eff were correlated positively with the value of Ki67 expression. Some previous studies [34,35] have found that the NIC values were positively correlated with Ki67 expression in rectal and gastric cancer. One possible explanation for these findings may be that highproliferating tumors are more heterogeneous, such as breast cancer, and are characterized by complex changes, Fig.…”

Section: Discussionmentioning

confidence: 80%

Dual-energy CT quantitative parameters for evaluating Immunohistochemical biomarkers of invasive breast cancer

et al. 2021

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Background Estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki67 are the most useful immunohistochemical biomarkers of invasive breast cancer. The purpose of this study is to investigate the possibility of quantitative parameters derived from dual-energy CT (DECT) to discriminate immunohistochemical biomarkers of invasive breast cancer. Methods This prospective study enrolled 120 patients with invasive breast cancer who underwent preoperative contrast-enhanced DECT for staging purposes from June 2019 to January 2020. DECT quantitative parameters, including normalized iodine concentration (NIC), the slope of the spectral Hounsfield unit curve (λHu), and the normalized effective atomic number (nZeff), were obtained from reconstructed images. DECT quantitative parameters were compared with the expression status, and the correlations with the value of immunohistochemical biomarkers were evaluated. Inter-observer reproducibility analysis was performed to assess the measurement reproducibility of quantitative parameters. The diagnostic performance of the quantitative parameters was analyzed by receiver operating characteristic curve. Results The ER-negative group tended to display higher venous phase NIC and nZeff compared with the ER-positive group (individually, p = 0.003, 0.011; area under the curve [AUC] of 0.65, 0.60). The PR-negative group demonstrated higher arterial and venous phase NIC compared with the PR-positive group (individually, p = 0.022, 0.005; AUC of 0.63, 0.65). NIC was correlated negatively with the value of ER and PR expression (r = − 0.175 ~ − 0.265, p = 0.002 ~ 0.042). The HER2-positive group tended to display higher venous phase nZeff than the HER2-negative group (p = 0.022; AUC of 0.59). The Ki67 high-proliferation group demonstrated higher arterial phase, venous phase NIC and nZeff than the Ki67 low-proliferation group (p < 0.001 ~ 0.005; AUC of 0.67 ~ 0.75). Both the NIC and nZeff were correlated positively with the value of Ki67 (r = 0.240 ~ 0.490, p < 0.001 ~ 0.014). Conclusions NIC and nZeff derived from DECT could be used to discriminate expression status and may associate with the value of immunohistochemical biomarkers of invasive breast cancer.

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“…While Al-Najami et al [ 51 ] found no significant differences in IC when comparing malignant with benign rectal tumors, Sun et al [ 52 ] demonstrated significant differences in IC between colonic adenomas and adenocarcinomas. Additionally, three studies determined positive correlations with other paraclinical measures such as perfusion computed tomography (CT) parameters (blood flow, blood volume, permeability, mean transit time), immunohistochemical evaluation of Ki-67 and HIF-1α levels as well as microsatellite stability and instability [ 53 , 54 , 55 ] (Studies shown in Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, IC normalization reference points varied among included studies, with the most common reference being the aorta. However, multiple studies have used other arteries such as the external iliac artery [ 53 , 55 ] as well as the psoas muscle as reference [ 48 ], making comparison of nIC between studies a challenge.…”

Section: Discussionmentioning

confidence: 99%

Gastrointestinal Applications of Iodine Quantification Using Dual-Energy CT: A Systematic Review

Taudorf

Ulriksen

et al. 2020

Diagnostics

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Dual-energy computed tomography (DECT) can estimate tissue vascularity and perfusion via iodine quantification. The aim of this systematic review was to outline current and emerging clinical applications of iodine quantification within the gastrointestinal tract using DECT. The search was conducted with three databases: EMBASE, Pubmed and The Cochrane Library. This identified 449 studies after duplicate removal. From a total of 570 selected studies, 30 studies were enrolled for the systematic review. The studies were categorized into four main topics: gastric tumors (12 studies), colorectal tumors (8 studies), Crohn’s disease (4 studies) and miscellaneous applications (6 studies). Findings included a significant difference in iodine concentration (IC) measurements in perigastric fat between T1–3 vs. T4 stage gastric cancer, poorly and well differentiated gastric and colorectal cancer, responders vs. non-responders following chemo- or chemoradiotherapy treatment among cancer patients, and a positive correlation between IC and Crohn’s disease activity. In conclusion, iodine quantification with DECT may be used preoperatively in cancer imaging as well as for monitoring treatment response. Future studies are warranted to evaluate the capabilities and limitations of DECT in splanchnic flow.

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Correlations between the iodine concentrations from dual energy computed tomography and molecular markers Ki-67 and HIF-1α in rectal cancer: A preliminary study

Cited by 22 publications

References 29 publications

Expression of GLUT3 and HIF-1α in Meningiomas of Various Grades Correlated with Peritumoral Brain Edema

Expression of GLUT3 and HIF-1α in Meningiomas of Various Grades Correlated with Peritumoral Brain Edema

Dual-energy CT quantitative parameters for evaluating Immunohistochemical biomarkers of invasive breast cancer

Gastrointestinal Applications of Iodine Quantification Using Dual-Energy CT: A Systematic Review

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