Corridos & Calaveras

A gene with homology to myogenin is expressed in developing myotomal musculature of the rainbow trout and in vitro during the conversion of myosatellite cells to myotubes

“…A family of cell type-specific basic helix-loop-helix (b-HLH) proteins, which includes MyoD [1], myogenin [2,3], Myf-5 [4], and MRF4/herculin/Myf-6 [5][6][7] has been shown to play an important role in the regulation of myogenesis. These proteins are nuclear phosphoproteins that activate muscle specific transcription through binding to a DNA consensus sequence known as an E-box found in the control regions of numerous muscle genes [8]. Each of these proteins has the ability to induce skeletal muscle phenotype in a wide range of cell types, when it is constitutively synthetized from transfected expression vectors [8].…”

“…These proteins are nuclear phosphoproteins that activate muscle specific transcription through binding to a DNA consensus sequence known as an E-box found in the control regions of numerous muscle genes [8]. Each of these proteins has the ability to induce skeletal muscle phenotype in a wide range of cell types, when it is constitutively synthetized from transfected expression vectors [8]. From genetic knockout experiments, it appears that either MyoD or Myf5 is required for the determination of skeletal myoblast [9], while Myogenin has a function in the transition from a determined myoblast to a fully differentiated myotube [10,11].…”