Corrigendum: A miRNA-145/TGF-β1 negative feedback loop regulates the cancer-associated fibroblast phenotype

Melling, Genevieve E; Flannery, Sarah E; Abidin, Siti Amalina Inche Zainal; Clemmens, Hannah; Prajapati, Priyanka; Hinsley, Emma E.; Hunt, Stuart; Catto, James W.F.; Coletta, Ricardo D.; Mellone, Massimiliano; Thomas, Gareth J.; Parkinson, Eric K.; Prime, Stephen S.; Paterson, Ian C.; Buttle, David J.; Lambert, D

doi:10.1093/carcin/bgy083

Cited by 2 publications

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“…Melling et al. ( 2018 ) propose a similar negative feedback loop as a regulator of the cancer-associated fibroblast phenotype.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis

Wong,

Mançanares,

Navarrete

et al. 2024

Veterinary Quarterly

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Mare endometrosis is a major reproductive problem associated with low fertility and is characterized by persistent inflammation, TGFβ-1 signaling, and consequently, extracellular matrix deposition, which compromises endometrial glands. Mesenchymal stem cell-based products (MSCs), such as extracellular vesicles (EVs), have gained attention due to the regulatory effects exerted by their miRNA cargo. Here, we evaluated the impact of preconditioning equine adipose mesenchymal stem cells with TGFβ-1 for short or long periods on the anti-fibrotic properties of secreted extracellular vesicles. MSCs were isolated from six healthy horses and exposed to TGFβ-1 for 4, 24, and 0 h. The expression of anti-fibrotic and pro-fibrotic miRNAs and mRNAs in treated cells and miRNAs in the cargo of secreted extracellular vesicles was measured. The resulting EVs were added for 48 h to endometrial stromal cells previously induced to a fibrotic status. The expression of anti-fibrotic and pro-fibrotic genes and miRNAs was evaluated in said cells using qPCR and next-generation sequencing. Preconditioning MSCs with TGFβ-1 for 4 h enriched the anti-fibrotic miRNAs (mir29c, mir145, and mir200) in cells and EVs. Conversely, preconditioning the cells for 24 h leads to a pro-fibrotic phenotype overexpressing mir192 and mir433. This finding might have implications for developing an EV-based protocol to treat endometrial fibrosis in mares.

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“…Melling et al. ( 2018 ) propose a similar negative feedback loop as a regulator of the cancer-associated fibroblast phenotype.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis

Wong,

Mançanares,

Navarrete

et al. 2024

Veterinary Quarterly

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Epigenetic modulation of the tumor microenvironment in head and neck cancer: Challenges and opportunities

Santos

Wagner

Ramos

et al. 2021

Critical Reviews in Oncology/Hematology

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Corrigendum: A miRNA-145/TGF-β1 negative feedback loop regulates the cancer-associated fibroblast phenotype

Cited by 2 publications

References 0 publications

Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis

Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis

Epigenetic modulation of the tumor microenvironment in head and neck cancer: Challenges and opportunities

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