Corrigendum: Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3

Guzmán-Verri, Caterina; Suárez-Esquivel, Marcela; Ruíz-Villalobos, Nazareth; Zygmunt, Michel S.; Gonnet, Mathieu; Campos, Elena; Víquez-Ruiz, Eunice; Aragón-Aranda, Beatriz; Conde-Álvarez, Raquel; Moriyón, Ignacio; Blasco, J.M.; Muñoz, Pilar; Baker, Kate S.; Thomson, Nicholas R.; Cloeckaert, Axel; Moreno, Edgardo

doi:10.3389/fvets.2019.00253

Cited by 4 publications

(7 citation statements)

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“…From the genomic and bacteriological perspectives, the pathogenic Brucella sp. BCCN84.3 strain belonged to the same species and, from now on, the type strain named B. nosferati BCCN84.3, the first species of this group isolated in Costa Rica ( 35 , 36 ).…”

Section: Resultsmentioning

confidence: 87%

“…Since the bacterium can invade the salivary glands of bats, it is therefore feasible that B. nosferati could be transmitted between vampire bats and also to domestic and wildlife species, including humans. The B. nosferati BCCN84.3 strain causing dog orchiepididymitis ( 35 , 36 ) supports this concept.…”

Section: Discussionmentioning

confidence: 91%

“…The bacteriological characterization showed that the bat isolates were smooth brucellae resembling the pathogenic Brucella sp. BCCN84.3 isolated from a dog orchiepididymitis case in Costa Rica ( 35 , 36 ), except that the Brucella of the bat strains were dominant M type while the BCCN84.3 strain was dominant A type ( Table 3 ). The dominant M profile of the bat isolates was confirmed through SDS-PAGE/Western blotting of the LPSs using B. microti (dominant M) and B. abortus (dominant A) LPSs as controls.…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, a high seroprevalence in wild peccaries and capybaras was found in a tropical region of Venezuela with the concomitant isolation of organisms typed as B. suis using classical bacteriology ( 50 , 53 ). However, conventional bacteriological typing cannot distinguish B. suis from B. nosferati ( 35 ). The closeness to areas with vampire bat attacks ( 40 , 54 ) and the absence of pigs ( B. suis reservoirs) in the vicinities where these sylvatic bacterial species were isolated ( 26 , 50 , 53 ) suggest the presence of zoonotic Brucella organisms in these tropical South American areas.…”

Section: Discussionmentioning

confidence: 99%

“…A biochemical profile was evaluated using the automated Vitek 2 System (bioMérieux) with the GN ID card to identify Gram-negative bacilli following the protocols described by the manufacturer. The vampire bat Brucella isolates and reference Brucella species, grown and harvested under the same conditions ( Dataset S2 ), were examined by whole-cell MALDI-TOF proteomics and gas–liquid chromatography/ms (glc/ms) of the derived FAMEs as described before ( 35 , 69 , 70 ).…”

Section: Methodsmentioning

confidence: 99%

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Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals

Hernández-Mora,

Chacón-Díaz,

Moreira-Soto

et al. 2023

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Desmodus rotundus, vampire bats, transmit dangerous infections, and brucellosis is a hazardous zoonotic disease, two adversities that coexist in the subtropical and tropical areas of the American continent. Here, we report a 47.89% Brucella infection prevalence in a colony of vampire bats inhabiting the tropical rainforest of Costa Rica. The bacterium induced placentitis and fetal death in bats. Wide-range phenotypic and genotypic characterization placed the Brucella organisms as a new pathogenic species named Brucella nosferati sp. nov., isolated from bat tissues, including the salivary glands, suggesting feeding behavior might favor transmission to their prey. Overall analyses placed B. nosferati as the etiological agent of a reported canine brucellosis case, demonstrating its potential for infecting other hosts. To assess the putative prey hosts, we analyzed the intestinal contents of 14 infected and 23 non-infected bats by proteomics. A total of 54,508 peptides sorted into 7,203 unique peptides corresponding to 1,521 proteins were identified. Twenty-three wildlife and domestic taxa, including humans, were foraged by B. nosferati -infected D. rotundus , suggesting contact of this bacterium with a broad range of hosts. Our approach is appropriate for detecting, in a single study, the prey preferences of vampire bats in a diverse area, demonstrating its suitability for control strategies where vampire bats thrive. IMPORTANCE The discovery that a high proportion of vampire bats in a tropical area is infected with pathogenic Brucella nosferati and that bats forage on humans and many wild and domestic animals is relevant from the perspective of emerging disease prevention. Indeed, bats harboring B. nosferati in their salivary glands may transmit this pathogenic bacterium to other hosts. This potential is not trivial since, besides the demonstrated pathogenicity, this bacterium possesses all the required virulent arsenal of dangerous Brucella organisms, including those that are zoonotic for humans. Our work has settled the basis for future surveillance actions in brucellosis control programs where these infected bats thrive. Moreover, our strategy to identify the foraging range of bats may be adapted for exploring the feeding habits of diverse animals, including arthropod vectors of infectious diseases, and therefore of interest to a broader audience besides experts on Brucella and bats.

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Section: Resultsmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals

Hernández-Mora,

Chacón-Díaz,

Moreira-Soto

et al. 2023

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Brucella

Ashford

Whatmore

2022

Molecular Typing in Bacterial Infections, Volume II

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Brucellatransmission from domestic and wild animals to dromedary camel: Diagnostic methods and zoonotic threats – A review

Mammeri

2022

Open Veterinary Science

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The present review discusses the transmission risk factors of camel (Camelus dromedarius) brucellosis in the limits of domestic and wild interfaces and zoonotic threats. The median position of the dromedary’s life between wild and the domestic areas seems to increase the risks of brucellosis transmission, compared to other receptive domestic ruminants. In arid environments, canids, lagomorphs, rodents, and wild boars are potential reservoirs of Brucella spp. Dromedary camels raised according to a periurban breeding system are often in direct or indirect contact with wild animals, domestic animals, and humans. Constraints of brucellosis detection and control in wild animals, especially in developing countries, hamper preventing disease in camelids and related occupational categories. A total eradication of animal and human brucellosis, in developing countries, is faced by the difficulty of applying quarantine periods for suspected animals, the lack of reliable diagnostic tools, and the impossibility of controlling animals at the common grazing lands, livestock markets, and transboundary areas. In developing countries, the informal she-camel milk collection, the periurban camel breeding, and the shortage in the technological processing and the pasteurization of she-camel milk play a key role in brucellosis zoonotic transmission.Veterinarians should have more initiative in brucellosis control in both domestic and wildlife animals.

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Corrigendum: Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3

Cited by 4 publications

References 0 publications

Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals

Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals

Brucella

Brucellatransmission from domestic and wild animals to dromedary camel: Diagnostic methods and zoonotic threats – A review

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