Corrigendum to “Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing<i> Klebsiella pneumoniae</i> Compared to Nonproducing Isolates”

Gharrah, Mustafa Muhammad; El-Mahdy, Areej M.; Barwa, Rasha

doi:10.1155/2018/1023076

Cited by 13 publications

(27 citation statements)

References 2 publications

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“…Our results are in line with other studies which PER-1 positive isolates produce more biofilm than PER-1 negative ones [30]. Moreover, Gharrah et al [31] indicated that ESBL producer strains form more biofilm than non-ESBL producers in K.pneumoniae. The uptake of ESBL carrying plasmids seemed to trigger the virulence factor by upregulation of some genes or adding new virulence genes such as type 1 and type 3 fimbriae resulting in greater invasion and biofilm forming abilities in K.pneumoniae [32].…”

Section: Discussionsupporting

confidence: 93%

Comparison of biofilm formation and efflux pumps in ESBL and carbapenemase producing Klebsiella pneumoniae

Yazgan

Türkel

Güçkan

et al. 2018

J Infect Dev Ctries

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Introduction: Klebsiella pneumoniae is an opportunistic pathogen that causes a range of diseases. The appearance of extended-spectrum β-lactamase -and carbapenemase-producing strains, in addition to the biofilm-forming phenotype, is a major problem in the clinical environment. Methodology: A total of 33 clinical K. pneumoniae isolates were used in this study. Antimicrobial susceptibilities were assessed by a disc diffusion assay. Biofilm formation was determined by a microtiter plate assay, staining with 1% crystal violet and measuring absorbance after destaining. Moreover, expression of acrA, kdeA, ketM, kpnEF, and kexD efflux associated genes was measured by qRT-PCR. Results: Isolates displayed high resistance to β-lactams such as cefazolin, cefuroxime, ceftriaxone, cefepime, piperacillin-tazobactam, imipenem, and meropenem and decreased resistance to gentamicin, amikacin, ciprofloxacin, and levofloxacin. ESBL-producing isolates formed more biofilm than carbapenemase-producing isolates. The mRNA expression levels in KPC isolates for acrA (2-fold), kdeA (2.7-fold), ketM (2.2-fold), and kpnEF (3.4-fold) were significantly increased compared to ESBL-producing isolates. There was no significant difference in kexD expression level. Conclusions: Under the conditions used here ESBL-producing isolates formed more biofilm than KPC postive isolates; this was associated with virulence determinants which were also transferred by plasmids together with ESBLs enzymes. Moreover, the upregulation of acrA, kdeA, ketM, and kpnEF efflux pumps was seen in carbapenemase-producing isolates demonstrating that high expression of efflux pumps alone could not confer resistance but may act as a physiological determinant such as bacterial pathogenicity and virulence, and cell-to-cell communication for bacteria.

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Section: Discussionsupporting

confidence: 93%

Comparison of biofilm formation and efflux pumps in ESBL and carbapenemase producing Klebsiella pneumoniae

Yazgan

Türkel

Güçkan

et al. 2018

J Infect Dev Ctries

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“…The vast credentials to acquire multiple antibiotic resistance mechanisms and express virulence traits scrupulously have led Klebsiella pneumoniae to attain medical importance amongst other opportunistic pathogens (1). Amidst antibiotic resistance mechanisms, high prevalence of extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (AmpCs) is the widely known concern (2).…”

Section: Introductionmentioning

confidence: 99%

“…The ability of K. pneumoniae to cause various infections is also attributed to the expression of arrays of virulence genes. In fact, published literature suggests that synchronization between antibiotic resistance and virulence traits may lead to the treatment failure in K. pneumoniae and E. coli related infections (1). Though emergence of resistant bacteria and evolution of pathogenetic mechanism may occur at different times however, it has been postulated that both the processes at the biological point of view are necessary for any bacteria to survive in different environments.…”

Section: Introductionmentioning

confidence: 99%

“…The distribution of K. pneumoniae is related to geographical regions and the type of infections. Moreover, the third generation cephalosporins are therapeutic agents crucial for the treatment of severe infections caused by K. pneumoniae therefore, studying of both processes might provide better understanding of the relationship between β-lactam resistance and virulence (1). Thus, we aimed to assess virulence traits of K. pneumoniae, and the production of ESBL or AmpC so as to gain an insight into any relation between antibiotic resistance and virulence profile.…”

Section: Introductionmentioning

confidence: 99%

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Virulence characterization of Klebsiella pneumoniae and its relation with ESBL and AmpC beta-lactamase associated resistance

Soltani¹,

Hasani²,

Rezaee³

et al. 2020

IJM

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Background and Objectives: Trend analysis reveals that Klebsiella pneumoniae has witnessed a steep enhancement in the antibiotic resistance and virulence over the last few decades. The present investigation aimed at a comprehensive approach investigating antibiotic susceptibility including, extended spectrum beta-lactamase (ESBL) and AmpC β-lactamase (AmpC) resistance and the prevalence of virulence genes among the K. pneumoniae isolates. Materials and Methods: Sixty-one K. pneumoniae isolates were obtained from various clinical infections. Antimicrobial susceptibility was performed by disk diffusion method. The Mast® D68C test detected the presence of ESBLs and AmpCs phenotypically, and later presence of ESBL and AmpC genes was observed by polymerase chain reaction (PCR). Multi- plex-PCR was performed to investigate various virulence genes. CMY-2 Results: Amongst 61 K. pneumoniae isolates, 59% were observed as ESBL and 14.7% as AmpC producers. All ESBL CTX-M-15 producers were positive for bla CTX-M-15 , while bla CTX-M-14 was observed in 54.1% isolates. The frequency of AmpC genes was CMY-2 as follows: bla CMY-2 (60.7%) and bla DHA-1 (34.4%). The most frequent virulence genes were those encoding enterobactin and DHA-1 lipopolysaccharide. Presence of mrkD was associated with bla CMY-2 DHA-1 gene, while bla significantly (p≤0.05) correlated DHA-1 with the presence of iutA and rmpA virulence genes. bla positive isolates had urine as a significant source, while bla positive isolates were mainly collected from wound exudates (p≤0.05). Conclusion: Our results highlight that ESBL and AmpC production along with a plethora of virulence trait on K. pneumoni- ae should be adequately considered to assess its pathogenesis and antibiotic resistance.

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“…Strains exposed to ceftazidime showed 20‐50% survival as compared to non‐exposed strains. This was not expected since resistance genes SHV and CTX‐M were detected and are known to confer extended spectrum β‐lactamase resistance phenotype .…”

Section: Discussionmentioning

confidence: 99%

spoT‐mediated stringent response influences environmental and nutritional stress tolerance, biofilm formation and antimicrobial resistance in Klebsiella pneumoniae

Davis

Brown

2019

APMIS

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Klebsiella pneumoniae is an important opportunistic pathogen with significant potential for virulence and multidrug resistance. Treatment failure often occurs because the pathogen may couple virulence and drug resistance with the stringent response. This study assessed the role of the spoT gene in environmental and nutritional stress tolerance, exopolysaccharide capsule production and biofilm formation. spoT mutants were constructed using the lambda red recombinase technique, and mutant and wild‐type (WT) strains were exposed to limiting concentrations of carbon (glucose), phosphate and aminoacid, and environmental stresses of ethanol, salt and heat. Cell viability, capsule production and cell length were assessed as well as the ability to grow biofilm under antibiotic pressure using gentamicin and ceftazidime. spoT mutants were more susceptible to stresses versus WT; the reverse was true for survival during biofilm susceptibility assay (p < 0.05), especially when carbon and phosphate were present. spoT mutants were elongated and lacked a capsule versus WT and non‐starved strains. The inability to produce capsule in mutants before and after starvation was likely a general effect of spoT mutation. These data suggest that the spoT‐mediated stringent response is important for K. pneumoniae in conditions of nutrient limitation, environmental stress and antimicrobial pressure.

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Corrigendum to “Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates”

Cited by 13 publications

References 2 publications

Comparison of biofilm formation and efflux pumps in ESBL and carbapenemase producing Klebsiella pneumoniae

Comparison of biofilm formation and efflux pumps in ESBL and carbapenemase producing Klebsiella pneumoniae

Virulence characterization of Klebsiella pneumoniae and its relation with ESBL and AmpC beta-lactamase associated resistance

spoT‐mediated stringent response influences environmental and nutritional stress tolerance, biofilm formation and antimicrobial resistance in Klebsiella pneumoniae

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