Corrigendum to “BBS9 gene in nonsyndromic craniosynostosis: Role of the primary cilium in the aberrant ossification of the suture osteogenic niche” [Bone 112 (July 2018) 58–70]

Barba, Marta; Pietro, Lorena Di; Massimi, Luca; Mc, Geloso; Frassanito, Paolo; Caldarelli, Massimo; Michetti, Fabrizio; Longa, Stefano Della; Pa, Romitti; C, Di Rocco; Arcovito, Alessandro; Parolini, Ornella; Tamburrini, Giampiero; Bernardini, Camilla; Sa, Boyadjiev; Lattanzi, Wanda

doi:10.1016/j.bone.2019.02.004

Cited by 1 publication

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“…R SQ Blood DNA Kit (Omega BioTek), according to the manufacturer's instructions. Total RNA samples were collected by TRIzol reagent method (Invitrogen), using the standard protocol (Di Pietro et al, 2017;Barba et al, 2018). gDNA and RNA purity and concentration were assessed using a UV spectrophotometer (DU 800, Beckman Coulter).…”

Section: Nucleic Acids Extractionmentioning

confidence: 99%

Graphene Oxide Nano-Concentrators Selectively Modulate RNA Trapping According to Metal Cations in Solution

Palmieri

Pietro

Perini

et al. 2020

Front. Bioeng. Biotechnol.

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With recent advances in nanotechnology, graphene nanomaterials are being translated to applications in the fields of biosensing, medicine, and diagnostics, with unprecedented power. Graphene is a carbon allotrope derived from graphite exfoliation made of an extremely thin honeycomb of sp2 hybridized carbons. In comparison with the bulk materials, graphene and its water-soluble derivative graphene oxide have a smaller size suitable for diagnostic platform miniaturization as well as high surface area and consequently loading of a large number of biological probes. In this work, we propose a nanotechnological method for concentrating total RNA solution and/or enriching small RNA molecules. To this aim, we exploited the unique trapping effects of GO nanoflakes in the presence of divalent cations (i.e., calcium and magnesium) that make it flocculate and precipitate, forming complex meshes that are positively charged. Here, we demonstrated that GO traps can concentrate nucleic acids in the presence of divalent cations and that small RNAs can be selectively released from GO-magnesium traps. GO nano-concentrators will allow better analytical performance with samples available in small amounts and will increase the sensitivity of sequencing platforms by short RNA selection.

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Section: Nucleic Acids Extractionmentioning

confidence: 99%

Graphene Oxide Nano-Concentrators Selectively Modulate RNA Trapping According to Metal Cations in Solution

Palmieri

Pietro

Perini

et al. 2020

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

Corrigendum to “BBS9 gene in nonsyndromic craniosynostosis: Role of the primary cilium in the aberrant ossification of the suture osteogenic niche” [Bone 112 (July 2018) 58–70]

Abstract: The authors regret that the affiliation of Alessandro Arcovito was mistakenly numbered as " c,g " on this publication.

Cited by 1 publication

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Graphene Oxide Nano-Concentrators Selectively Modulate RNA Trapping According to Metal Cations in Solution

Graphene Oxide Nano-Concentrators Selectively Modulate RNA Trapping According to Metal Cations in Solution

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