Corrigendum to “Development of IgY-Based Sandwich ELISA as a Robust Tool for Rapid Detection and Discrimination of Toxigenic <i>Vibrio cholera</i>”

Bayat, Mahdiye; Khabiri, Alireza; Hemati, Behzad

doi:10.1155/2019/4164982

Cited by 4 publications

(3 citation statements)

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“…In the current research, Bayat et al used IgY based on ELISA for the detection of toxigenic V. cholerae for the first time. They reported the limit of detection of OmpW is 10 3 CFU/ml and that of CtxB is nearby 33 pg/ml ( 34 ) University of East Anglia developed a colorimetric bioassay method for Cholera toxin (CTB) with a limit detection of 54 nM ( 35 ). Lee et al also used the whole-cell SELEX strategy to generate aptamers with high affinity against live Listeria monocytogenes .…”

Section: Discussionmentioning

confidence: 99%

Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1

Mojarad¹,

Gargaria²

2020

IJM

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Background and Objectives: In recent years, the prevalence of diseases caused by Vibrio spp. is increasing in the world, and among them species, Vibrio cholerae is the most important Vibrio associated with pandemic and epidemic cholera outbreaks. Therefore, the development of a reliable method for early and accurate detection of V. cholerae for management of diseases is a real need. Aptamers with the ability to detect targets with high specificity and accuracy can be one of the candidates used for the whole cell and thereby V. cholerae detection. Materials and Methods: In this research high-affinity DNA aptamers against with two major serotypes of Inaba (ATCC 39315) and Ogawa (clinical sample) were selected from DNA aptamer library through 12 rounds of Systematic Evolution of Ligands by Exponential (SELEX) enrichment procedure using live cells as a target which monitored with flow cytometry. Results: The binding efficiency and dissociation constant of the isolated aptamers V.ch47 and V.ch27 were 56.4%, 53.3% and 15.404 ± 4.776 pM, 20.186 ± 3.655 pM, respectively. A sandwich Enzyme-linked aptamer sorbent assay (ELASA) was developed with the biotinylated V.ch47 aptamer and our previously developed nanobody anti-Lipopolysaccharides (LPS). We optimized this system with V. cholerae O1 and analyzed their cross reactivity with close physiological bacteria. The threshold of detection was obtained 104 CFU/ml in the sandwich ELASA process. Conclusion: Our results showed that the sandwich ELASA is sensitive enough for the rapid detection of V. cholerae from other bacteria.

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Section: Discussionmentioning

confidence: 99%

Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1

Mojarad¹,

Gargaria²

2020

IJM

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“…The study proposed two sensitive and specific sandwich ELISA formats, with no cross-reactivity with other bacteria. It was suggested that these IgY based ELISAs could constitute a rapid and specific detection tool for assessing V. cholera in different biological samples (52).…”

Section: Igy An Established Immune-fighter -Is There Something New Onmentioning

confidence: 99%

IgY ‑ turning the page toward passive immunization in COVID-19 infection (Review)

Constantin

Neagu

Supeanu³

et al. 2020

Exp Ther Med

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The world is facing one of the major outbreaks of viral infection of the modern history, however, as vaccine development workflow is still tedious and can not control the infection spreading, researchers are turning to passive immunization as a good and quick alternative to treat and contain the spreading. Within passive immunization domain, raising specific immunoglobulin (Ig)Y against acute respiratory tract infection has been developing for more than 20 years. Far from being an obsolete chapter we will revise the IgY-technology as a new frontier for research and clinic. A wide range of IgY applications has been effectively confirmed in both human and animal health. The molecular particularities of IgY give them functional advantages recommending them as good candidates in this endeavor. Obtaining specific IgY is sustained by reliable and nature friendly methodology as an alternative for mammalian antibodies. The aria of application is continuously enlarging from bacterial and viral infections to tumor biology. Specific anti-viral IgY were previously tested in several designs, thus its worth pointing out that in the actual COVID-19 pandemic context, respiratory infections need an enlarged arsenal of therapeutic approaches and clearly the roles of IgY should be exploited in depth.

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“…Однако определение генов, кодирующих синтез холерного токсина (ХТ), не всегда указывает на наличие экспрессии самого токсина. Кроме того, в ряде ситуаций прямое выявление токсина остаётся предпочтительным, поскольку отмечен высокий уровень мутаций в локусе ctx, которые могут приводить к ложноотрицательным результатам [Ahn-Yoon et al, 2003;Tuteja et al, 2007; Петрова и др., 2009; Алексеева и др., 2019; Bayat et al, 2019]. В связи с этим одновременно используются и иммунологические методы с высокой чувствительностью и специфичностью, так как штаммы холерных вибрионов Эль Тор и серогруппы О139 отличаются низким уровнем продукции токсина in vitro [Shlyapnikov et al, 2012;Meza-Lucas et al, 2016].…”

Section: Introductionunclassified

Solid-phase enzyme-linked immunosorbent assay (direct option) for detecting toxigenic vibrio cholerae serogroups О1 and О139

Alekseeva¹,

Yakusheva²,

Evdokimova³

et al. 2022

Вест. ПГУ. Биол.

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As a result of the studies, the method of direct solid-phase enzyme immunoassay (ELISA test) was optimized for detecting toxigenic strains of Vibrio cholerae O1 and O139 serogroups based on monoclonal and polyclonal antitoxic immunoglobulin peroxidase conjugates. The study detected the following optimal parameters for the analysis: sensitization of a tablet by toxicodermia drugs diluted in phosphate-saline buffer (PBS) pH 7.4, up to titers of 1:2-1:4, their adsorption on the solid phase of the tablet for 2 hours at 37° C, blocking of free binding sites by the 1% solution of bovine serum albumin (BSA) for 30 minutes (at 37°C); contact time of the investigated sample with mono- or polyclonal conjugates peroxidase - 30 minutes (at 37°C). Tests on homologous and heterologous strains showed a high specificity of the enzyme immunoassay and the possibility of its use for detecting toxigenic Vibrio cholerae.

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Corrigendum to “Development of IgY-Based Sandwich ELISA as a Robust Tool for Rapid Detection and Discrimination of Toxigenic Vibrio cholera”

Cited by 4 publications

References 1 publication

Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1

Aptamer-nanobody based ELASA for detection of Vibrio cholerae O1

IgY ‑ turning the page toward passive immunization in COVID-19 infection (Review)

Solid-phase enzyme-linked immunosorbent assay (direct option) for detecting toxigenic vibrio cholerae serogroups О1 and О139

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