Corrigendum to ‘miR-145 inhibits invasion of bladder cancer cells by targeting PAK1’

Kou, Bo; Gao, Yang; Du, Chong; Shi, Qi; Xu, Shan; Wang, Chen-Qing; Wang, Xinyang; He, Dalin; Guo, Peng

doi:10.1016/j.urolonc.2022.05.009

Cited by 2 publications

(39 citation statements)

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“…All primers were synthesized by IDT (Coralville, IA, USA) and sequences of primers for RT-qPCR are provided in Table 1. The circular nature of circSTAG2 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25) was confirmed by Sanger sequencing across the back splicing junction site. All assays were independently repeated at least three times.…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 91%

“…CircSTAG2 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25) siRNA1 (rGrArUrGrArUrGrCrArUrArUrCrArGrGrUrArUrUr-GrUrCAA) and siRNA2 (rUrUrGrArCrArArUrArCrCrUrGrArUrArUrGrCrArUrCrArUr-CrUrU) were synthesized by IDT based on the sequence of mature circSTAG2 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25). Both siRNAs encompassed the back splicing junction area.…”

Section: Molecular Cloning and Cell Transfectionmentioning

confidence: 99%

“…One of the downstream miR-145-5p targets is TAGLN2, which is known to promote motility and invasion of BCa cells [20]. Therefore, we propose that circSTAG2 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25) can regulate cancer cell behavior via miR-145-5p and TAGLN2.…”

Section: Introductionmentioning

confidence: 97%

“…The current study characterized in detail one of the circRNAs derived from STAG2. CircSTAG2 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25) was differentially detected in urine derived from BCa patients and healthy volunteers. It further was found at higher levels in urine derived from patients with recurrent tumors as well as more abundant in higher tumorigenic BCa cell lines.…”

Section: Introductionmentioning

confidence: 99%

“…It further was found at higher levels in urine derived from patients with recurrent tumors as well as more abundant in higher tumorigenic BCa cell lines. Our results suggest that circSTAG2 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25) may regulate the behavior of cancer cells by sponging miR-145-5p, a microRNA that is known to possess tumor suppressor functions in BCa [19][20][21][22][23][24][25]. One of the downstream miR-145-5p targets is TAGLN2, which is known to promote motility and invasion of BCa cells [20].…”

Section: Introductionmentioning

confidence: 99%

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Circular STAG2 RNA Modulates Bladder Cancer Progression via miR-145-5p/TAGLN2 and Is Considered as a Biomarker for Recurrence

Du,

Waltzer,

Wilusz

et al. 2024

Cancers

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The current study aimed to elucidate the regulatory mechanisms of the circRNA hsa_circ_0139697 (circSTAG2(16–25)) in BCa and to consider the opportunity of using circSTAG2(16–25) isolated from BCa patient urine as a marker for disease development prediction. The selection of this circRNA was determined by the special role of its parental gene STAG2 in BCa biology. The circRNA hsa_circ_0139697 was chosen from 25 STAG2 circRNAs due to its differential expression in the urine of BCa patients and healthy volunteers. Higher levels of circSTAG2(16–25) were detected in urine samples obtained from patients with recurrent tumors. A higher expression of circSTAG2(16–25) was also detected in more tumorigenic BCa cell lines. The overexpression of circSTAG2(16–25) in BCa cells induced the elevation of proliferation, motility, and invasion. To study the mechanisms of circSTAG2(16–25) activity, we confirmed that circSTAG2(16–25) can bind miR-145-5p in vitro as was predicted by bioinformatic search. miR-145-5p was shown to suppress some genes that promoted BCa progression. One of these genes, TAGLN2, encodes the protein Transgelin 2, which plays a role in BCa cell motility and invasion. Therefore, the possible mechanism of action of circSTAG2(16–25) could be sponging the tumor suppressor miR-145-5p, which results in activation of TAGLN2. In addition, circSTAG2(16–25) might be considered as a potential biomarker for recurrence prediction.

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Section: Rna Extraction and Rt-qpcrmentioning

confidence: 91%

Section: Molecular Cloning and Cell Transfectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Circular STAG2 RNA Modulates Bladder Cancer Progression via miR-145-5p/TAGLN2 and Is Considered as a Biomarker for Recurrence

Du,

Waltzer,

Wilusz

et al. 2024

Cancers

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The Suppression of the Epithelial to Mesenchymal Transition in Prostate Cancer through the Targeting of MYO6 Using MiR-145-5p

Armstrong,

Willoughby,

McKenna

2024

IJMS

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Aberrant expression of miR-145-5p has been observed in prostate cancer where is has been suggested to play a tumor suppressor role. In other cancers, miR-145-5p acts as an inhibitor of epithelial-to-mesenchymal transition (EMT), a key molecular process for tumor progression. However, the interaction between miR-145-5p and EMT remains to be elucidated in prostate cancer. In this paper the link between miR-145-5p and EMT in prostate cancer was investigated using a combination of in silico and in vitro analyses. miR-145-5p expression was significantly lower in prostate cancer cell lines compared to normal prostate cells. Bioinformatic analysis of The Cancer Genome Atlas prostate adenocarcinoma (TCGA PRAD) data showed significant downregulation of miR-145-5p in prostate cancer, correlating with disease progression. Functional enrichment analysis significantly associated miR-145-5p and its target genes with EMT. MYO6, an EMT-associated gene, was identified and validated as a novel target of miR-145-5p in prostate cancer cells. In vitro manipulation of miR-145-5p levels significantly altered cell proliferation, clonogenicity, migration and expression of EMT-associated markers. Additional TCGA PRAD analysis suggested miR-145-5p tumor expression may be useful predictor of disease recurrence. In summary, this is the first study to report that miR-145-5p may inhibit EMT by targeting MYO6 in prostate cancer cells. The findings suggest miR-145-5p could be a useful diagnostic and prognostic biomarker for prostate cancer.

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Corrigendum to ‘miR-145 inhibits invasion of bladder cancer cells by targeting PAK1’

Cited by 2 publications

References 0 publications

Circular STAG2 RNA Modulates Bladder Cancer Progression via miR-145-5p/TAGLN2 and Is Considered as a Biomarker for Recurrence

Circular STAG2 RNA Modulates Bladder Cancer Progression via miR-145-5p/TAGLN2 and Is Considered as a Biomarker for Recurrence

The Suppression of the Epithelial to Mesenchymal Transition in Prostate Cancer through the Targeting of MYO6 Using MiR-145-5p

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