Cortactin-Binding Protein 90 (CBP90) Expression in the Mouse Mammary Glands during Prolactin-Induced Lobuloalveolar Development

Imaoka, Tatsuhiko; Horseman, Nelson D.; Lockefeer, Jason A.; Mori, Takao; Matsuda, Manabu

doi:10.2108/zsj.19.443

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…"Virtual" Northern blotting "Virtual" Northern blotting is an alternative method to conventional Northern blotting, in which full-length cDNA is used instead of RNA (Franz et al 1999), and it is useful especially when the amount of tissue sample is as small as that in the case of the developing genital tract. The procedure was basically the same as previously described (Imaoka et al 2002). The alkaline phosphatase-labeled DNA probes for CRBP1, CRBP2, cytokeratin 18 (K18), lecithin:retinol acyltransferase (LRAT), b-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were made from a plasmid insert that contained an RT-PCR product of each gene.…”

Section: Animals and Hormone Treatmentsmentioning

confidence: 99%

Neonatal estrogenization leads to increased expression of cellular retinol binding protein 2 in the mouse reproductive tract

Matsuda

Masui

Mori

2004

Cell and Tissue Research

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Exposure to estrogenic substances during a time window, the so-called "critical period," in perinatal life causes an irregular development of the genital tract that leads to ovary-independent proliferation and cornification in the vaginal epithelium in mice. We have previously demonstrated that retinol inhibits the irreversible effects of estrogen on the vagina. Here, mice kept in a vitamin-A-deficient condition during perinatal life were shown to be more sensitive to the harmful effects of estrogen. In addition, expression of mRNA for retinol binding protein type 2 (CRBP2), a "small intestine-specific" cytosolic protein that captures intracellular retinal and retinol, was detected in the vaginal epithelium. Induction of increased expression of CRBP2 mRNA by estrogen was also evident in the uterus and epididymis. Both estradiol-17beta and diethylstilbestrol markedly increased the tissue content of CRBP2 mRNA in the vagina and uterus during the neonatal "critical period" but not after 15 days of age. These results taken together imply that estrogen disrupts the local vitamin A balance by an induction of CRBP2 gene expression in the epithelium in the developing mouse genital tract, and that retinoid imbalance may contribute to the genesis of irreversible effects of estrogen on the vagina.

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Section: Animals and Hormone Treatmentsmentioning

confidence: 99%

Neonatal estrogenization leads to increased expression of cellular retinol binding protein 2 in the mouse reproductive tract

Matsuda

Masui

Mori

2004

Cell and Tissue Research

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Cortactin: The Gray Eminence of the Cytoskeleton

2006

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Cortactin, an actin filament-binding protein and target of multiple kinases, has emerged as a central element connecting signaling pathways with cytoskeleton restructuring. It is involved in a perplexingly diverse array of cellular processes, including cell motility, invasiveness, synaptogenesis, endocytosis, intercellular contact assembly, and host-pathogen interactions, where the common denominator appears to be a role in the coordination of membrane dynamics with cytoskeletal remodeling. Although in recent years our knowledge about cortactin has increased exponentially, the exact mechanisms underlying its fundamental roles remain to be defined.

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Aldolase C/Zebrin Gene Regulation by Prolactin During Pregnancy and Lactation

Matsuda

Lockefeer

Horseman

2003

ENDO

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Prolactin (PRL) is necessary for the genesis of mammary alveolar buds and for lactation. A cDNA library enriched for PRL-dependent genes was made by suppression subtractive hybridization. Aldolase C/zebrin (AldC/zebrin), a brain-specific aldolase, was found to be PRL-dependent in the mouse mammary glands. AldC/zebrin was preferentially expressed in the alveolar buds. Expression of the gene in the ovary was also evident. During pregnancy, mammary AldC/zebrin mRNA levels were elevated beginning at midpregnancy (d 10 of pregnancy) in accordance with the genesis of the lobuloalveolar system, and the expression level was gradually increased through the end of pregnancy. Lactating mammary gland contained a very high level of AldC/zebrin mRNA, and the gene expression decreased during involution. By contrast, levels of aldolase A and B mRNA expression in the mammary glands were less affected by pregnancy and lactation. The selective regulation of AldC/zebrin may contribute to a shift in nutrient metabolism during pregnancy and lactation to facilitate epithelial growth and biosynthesis of milk constituents.

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Cortactin-Binding Protein 90 (CBP90) Expression in the Mouse Mammary Glands during Prolactin-Induced Lobuloalveolar Development

Cited by 3 publications

References 22 publications

Neonatal estrogenization leads to increased expression of cellular retinol binding protein 2 in the mouse reproductive tract

Neonatal estrogenization leads to increased expression of cellular retinol binding protein 2 in the mouse reproductive tract

Cortactin: The Gray Eminence of the Cytoskeleton

Aldolase C/Zebrin Gene Regulation by Prolactin During Pregnancy and Lactation

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