Corticosteroids Modulate Angiogenic Soluble Factors in Ulcerative Colitis Patients

Pousa, Inés D.; Algaba, Alicia; Linares, Pablo M.; Sanz-Cameno, Paloma; Maté, José; Moreno–Otero, Ricardo; Bermejo, Fernando; Gisbert, Javier P.

doi:10.1007/s10620-010-1327-x

Cited by 8 publications

(5 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relationship between RSV and PI3K/Akt has been investigated previously; for instance, RSV was found to downregulate the protein expression of PI3K/Akt, thus attenuating the progression of intestine ( Radwan and Karam, 2020 ). Moreover, upregulated levels of VEGFA have been documented in UC patients compared to normal controls ( Pousa et al, 2011 ). Similarly, RSV possesses the ability to inhibit the expression of CXCR4 by reducing the phosphorylation level of NF-κB, which brings about a reduction in VEGF secretion ( Seong et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, VEGFA can influence physiology and tumor-induced angiogenesis, whereas elevated VEGFA gene expressions are associated with tumor progression, recurrence, and survival ( Andreozzi et al, 2014 ). More strikingly, UC patients are known to exhibit higher levels of VEGFA than normal controls ( Pousa et al, 2011 ). Consequently, the current study aimed to verify how RSV regulates the PI3K/Akt/VEGFA pathway to relieve UC.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway

Zhu

Zheng

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Ulcerative colitis (UC) is a chronic inflammatory disease that affects the colon, and its incidence is on the rise worldwide. Resveratrol (RSV), a polyphenolic compound, was recently indicated to exert anti-inflammatory effects on UC. Consequently, the current study was conducted to investigate the mechanism of RSV on alleviating UC in mice by mediating intestinal microflora homeostasis. First, potential targets that RSV may regulate UC were screened using the TCMSP database. Next, mice were treated differently, specifically subjected to sham-operation and dextran sulfate sodium (DSS) induction, and then treated or untreated with RSV. Disease Activity Index (DAI) and Hematoxylin-Eosin (HE) staining were employed to analyze the pathological changes of mice colon. In addition, the expression patterns of inflammatory factors in spleen tissues were detected using ELISA, while the protein expression patterns of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and vascular endothelial growth factor A (VEGFA) in colon tissues were determined by means of immunohistochemistry (IHC) and Western blot analysis. Moreover, changes in intestinal flora and metabolite diversity in UC were analyzed by metabonomics. It was found that RSV played inhibitory roles in the PI3K/Akt pathway in mice. Meanwhile, the administration of RSV induced downregulated the expressions of TNF-α, IFN-γ, IL-1β, IL-6, and IL-4. The six floras of Haemophilus and Veillonella were significantly enriched in UC, while Clostridium, Roseburia, Akkermansia, and Parabacteroides were found to be enriched in control samples. Lastly, it was noted that Akkermansia could regulate the intestinal flora structure of UC mice through triacylglycerol biosynthesis, glycerol phosphate shuttle, cardiolipin biosynthesis, and other metabolic pathways to improve UC in mice. Altogether, our findings indicate that RSV suppressed the activation of the PI3K/Akt pathway and reduced the VEGFA gene expression to alleviate UC in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway

Zhu

Zheng

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…); 4) full text is not available and the abstract contains not enough data (ie, describes “IBD” without a specification for CD or UC); 5) cytokines under study are not in our list; or 6) the paper describes cytokine levels before/after certain treatment and contains no control group. The ultimate data contained 236 contributions on cytokines, chemokines, and growth factors derived from 89 research papers 7–11,29…”

Section: Resultsmentioning

confidence: 99%

Characterization of Serum Cytokine Profile in Predominantly Colonic Inflammatory Bowel Disease to Delineate Ulcerative and Crohn's Colitides

Korolkova

Myers

Pellom

et al. 2015

Clin Med Insights Gastroenterol

View full text Add to dashboard Cite

BACKGROUNDAs accessible diagnostic approaches fail to differentiate between ulcerative colitis (UC) and Crohn’s colitis (CC) in one-third of patients with predominantly colonic inflammatory bowel disease (IBD), leading to inappropriate therapy, we aim to investigate the serum cytokine levels in these patients in search of molecular biometric markers delineating UC from CC.METHODSWe measured 38 cytokines, chemokines, and growth factors using magnetic-bead-based multiplex immunoassay in 25 UC patients, 28 CC patients, and 30 controls. Our results are compared with those from a review of current literature regarding advances in serum cytokine profiles and associated challenges preventing their use for diagnostic/prognostic purposes.RESULTSUnivariate analysis showed statistically significant increases of eotaxin, GRO, and TNF-α in UC patients compared to controls (Ctrl); interferon γ, interleukin (IL)-6, and IL-7 in CC group compared to Ctrl; and IL-8 in both UC and CC versus Ctrl. No cytokines were found to be different between UC and CC. A generalized linear model identified combinations of cytokines, allowing the identification of UC and CC patients, with area under the curve (AUC) = 0.936, as determined with receiver operating characteristic (ROC) analysis.CONCLUSIONSThe current knowledge available about circulating cytokines in IBD is often contradictory. The development of an evidence-based tool using cytokines for diagnostic accuracy is still preliminary.

show abstract

“…Angiogenesis, the formation of new blood vessels, contributes to the neoplastic process and plays a role in the pathology of IBD (33). 5-ASA (mesalamine), a drug often used to treat IBD, in addition to effects of cyclooxygenase and lipoxygenase pathways, can alter the balance between angiogenic and antiangiogenic factors (32,43). In this study we examined the impact of modulation of the microflora on levels of the angiogenic factor VEGF and the antiangiogenic factors endostatin and angiostatin.…”

Section: Discussionmentioning

confidence: 99%

Pretreatment with the probiotic VSL#3 delays transition from inflammation to dysplasia in a rat model of colitis-associated cancer

Appleyard

Cruz

Isidro

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

108

View full text Add to dashboard Cite

Evidence supports involvement of microflora in the transition of chronic inflammation to neoplasia. We investigated the protective efficacy of the probiotic VSL#3 in a model of colitis-associated colorectal cancer. Chronic colitis was induced in Sprague-Dawley rats by administration of trinitrobenzene sulfonic acid (TNBS), followed 6 wk later by systemic reactivation. To induce colitis-associated dysplasia and cancer, the animals received TNBS (intravenously) twice a week for 10 wk. One group received VSL#3 in drinking water from 1 wk before colitis induction until death. The colons were examined for damage and presence of dysplasia or cancer. Samples were analyzed for cell proliferation and apoptosis, vitamin D receptor (VDR) expression, angiogenic factors, and presence of alkaline sphingomyelinase or phosphatase. Microbial community composition was evaluated by terminal restriction fragment-length polymorphism analysis of the bacterial 16S rRNA gene. None of the probiotic-treated animals developed carcinoma, and no high-grade dysplasia was found in either the proximal or mid colon. In contrast, 29% of the animals in the control group developed carcinoma in one or more regions of the colon. VSL#3-treated animals had significantly less damage than the vehicle treated-controls in all areas of the colon, and this correlated with decreased richness and diversity of the mucosally adherent microbiota. Treatment with the probiotic increased the antiangiogenic factor angiostatin, VDR expression, and alkaline sphingomyelinase. We concluded that pretreatment with the probiotic VSL#3 can attenuate various inflammatory-associated parameters, delaying transition to dysplasia and cancer, thus offering its potential therapeutic use in patients with long-standing colitis.

show abstract

Corticosteroids Modulate Angiogenic Soluble Factors in Ulcerative Colitis Patients

Cited by 8 publications

References 44 publications

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway

Characterization of Serum Cytokine Profile in Predominantly Colonic Inflammatory Bowel Disease to Delineate Ulcerative and Crohn's Colitides

Pretreatment with the probiotic VSL#3 delays transition from inflammation to dysplasia in a rat model of colitis-associated cancer

Contact Info

Product

Resources

About