Cortisol and cortisone analysis in serum and plasma by atmospheric pressure photoionization tandem mass spectrometry

Kushnir, Mark M.; Neilson, R.; Roberts, William L.; Rockwood, Alan L.

doi:10.1016/j.clinbiochem.2004.01.005

Cited by 112 publications

(73 citation statements)

References 24 publications

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“…Cortisol determinations are commonly used for diagnosis of Cushing's Syndrome, congenital adrenal hyperplasia, and adrenal insufficiency (Addison's disease). Increased cortisol secretion, altered cortisol metabolism, and increased tissue sensitivity to cortisol may be related to insulin resistance, obesity, and hypertension [1]. Human growth hormone (somatotropin, GH), secreted by the anterior pituitary, is essential to the processes of growth and metabolism [2].…”

Section: Introductionmentioning

confidence: 99%

Pediatric reference intervals for FSH, LH, estradiol, T3, free T3, cortisol, and growth hormone on the DPC IMMULITE 1000

Soldin

Hoffman

Waring

et al. 2005

Clinica Chimica Acta

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Background-We studied serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), triiodothyronine (T3), free T3 (FT3), cortisol and growth hormone (GH) concentrations in a population of pediatric patients. The reference intervals were determined separately for females and males stratified by age groups to assess age-and sex-related differences. Our objective was to obtain reference intervals for the 7 serum analytes for our pediatric population using the IMMULITE 1000 system. Methods-Serum samples of 800 in-and out-patients, newborn to 19 years old were analyzed using the DPC IMMULITE 1000 chemiluminescent immunoassay system.Results and conclusions-We report pediatric reference intervals for FSH, LH, E2, T3, FT3, cortisol, and GH. These reference intervals provide the basis for clinical interpretation of laboratory results using the IMMULITE 1000 system and the assessment of child development.

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Section: Introductionmentioning

confidence: 99%

Pediatric reference intervals for FSH, LH, estradiol, T3, free T3, cortisol, and growth hormone on the DPC IMMULITE 1000

Soldin

Hoffman

Waring

et al. 2005

Clinica Chimica Acta

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“…Plasma samples were analysed for Pregn, CORT, DHEA, A4, Te, PROG, E1, E2 and E3 using liquid chromatography tandem mass spectrometry methods (LC-MS/MS) as previously described [45][46][47]. Briefly, steroids were extracted from samples and CORT, DHEA, A4, Pregn, and PROG were derivatized with hydroxylamine to form oxime derivatives; E1, E2 and E3 were derivatized with dansyl chloride to form dansyl derivatives.…”

Section: Liquid Chromatography Tandem Mass Spectrometrymentioning

confidence: 99%

Domestication effects on behavioural and hormonal responses to acute stress in chickens

Ericsson

Fallahsharoudi

Bergquist

et al. 2014

Physiology & Behavior

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Comparative studies have shown that alterations in physiology, morphology and behaviour have arisen due to the domestication. A driving factor behind many of the changes could be a shift in stress responses, with modified endocrine and behavioural profiles. In the present study we compared two breeds of chicken (Gallus gallus), the domestic White Leghorn (WL) egg laying breed and its ancestor, the Red Junglefowl (RJF). Birds were exposed to an acute stress event, invoked by three or ten minutes of physical restraint. They were then continuously monitored for the effects on a wide range of behaviours during a 60 min recovery phase. Blood samples were collected from the chicken at baseline, and after 10 and 60 min following a similar restraint stress, and the samples were analyzed for nine endogenous steroids of the HPA and HPG axis. Concentration of the steroids was determined using validated liquid chromatography tandem mass spectrometry methods. In RJF, an immediate behavioural response was observed after release from restraint in several behaviours, with a relatively fast return to baseline within one hour. In WL, some behaviours were affected for a longer period of time, and others not at all. Concentrations of corticosterone increased more in RJF, but returned faster to baseline compared to WL. A range of baseline levels for HPG-related steroids differed between the breeds, and they were generally more affected by the stress in WL than in RJF. In conclusion, RJF reacted stronger both behaviourally and physiologically to the restraint stress, but also recovered faster. This would appear to be adaptive under natural conditions, whereas the stress recovery of domesticated birds has been altered by domestication and breeding for increased reproductive output.

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“…Despite structural similarities among steroids, the efficiency of soft ionization methods for their detection is very different. The ionization efficiency of each steroid must be studied and can be different using different ion sources and instruments (Janzen et al, 2008;Kushnir et al, 2004;Kushnir et al, 2006b;Vogeser et al, 2001a). Kushnir, for example, observed the highest absolute intensity for 17-hydroxyprogesterone with APPI followed by APCI and ESI.…”

Section: Methodological Aspects Of Lc-ms/msmentioning

confidence: 99%

“…highest specificity and precision from a single serum sample (Kushnir et al, 2004;Tenhola et al, 2005;Vogeser et al, 2001b). This allows the determination of cortisol cortisone ratios as a marker for 11β-HSD activity within a short turn-around time.…”

Section: Lc-ms/ms Enables the Simultaneous Determination Of Cortisol mentioning

confidence: 99%

Steroid measurement with LC–MS/MS in pediatric endocrinology

Rauh

2009

Molecular and Cellular Endocrinology

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Please cite this article as: Rauh, M., Steroid measurement with LC-MS/MS in pediatric endocrinology, Molecular and Cellular Endocrinology (2008), doi:10.1016/j.mce.2008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Cortisol and cortisone analysis in serum and plasma by atmospheric pressure photoionization tandem mass spectrometry

Cited by 112 publications

References 24 publications

Pediatric reference intervals for FSH, LH, estradiol, T3, free T3, cortisol, and growth hormone on the DPC IMMULITE 1000

Pediatric reference intervals for FSH, LH, estradiol, T3, free T3, cortisol, and growth hormone on the DPC IMMULITE 1000

Domestication effects on behavioural and hormonal responses to acute stress in chickens

Steroid measurement with LC–MS/MS in pediatric endocrinology

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