Cortisol modulates inflammatory responses in LPS-stimulated RAW264.7 cells via the NF-κB and MAPK pathways

Dong, Junsheng; Li, Jianji; Cui, Luying; Wang, Yefan; Lin, Jiaqi; Qu, Yang; Wang, Heng

doi:10.1186/s12917-018-1360-0

Cited by 54 publications

(45 citation statements)

References 37 publications

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“…Stress hormones serve as beacons that make the immune system ready for potential challenges perceived by the brain. Nevertheless, the homeostasis of this system may break down if the enhanced immune response is dysregulated after long activation, as seen during chronic stress [ 32 , 33 , 41 , 42 ].…”

Section: Neuroendocrine Responses To Stressmentioning

confidence: 99%

Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review

Alhussien

Dang

2020

Vet World

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Dairy cows are exposed to various stressors during their production cycle that makes them more susceptible to various diseases. Phagocytes (neutrophils and macrophages) are important soldiers of the innate immune system. Neutrophils are the first responders to an inflammatory response and stress and kill pathogens by generating reactive oxygen species and by the release of various antimicrobial peptides, enzymes, neutrophil extracellular trap formation, etc. Macrophages, the other phagocytes, are also the cleanup crew for the innate immune system that removes debris, pathogens, and dead neutrophils later on after an inflammatory response. The neuroendocrine system along with phagocytes exhibits an immunomodulatory potential during stressful conditions. Neuroendocrine system directly affects the activity of phagocytes by communicating bidirectionally through shared receptors and messenger molecules such as hormones, neurotransmitters, or cytokines. Different immune cells may show variable responses to each hormone. Short time exposure to stress can be beneficial, but repeated or extended exposure to stress may be detrimental to the overall health and well-being of an animal. Although some stresses associated with farming practices in dairy cows are unavoidable, better understanding of the interactions occurring between various stress hormones and phagocytic cells can help to reduce stress, improve productivity and animal welfare. This review highlights the role played by various stress hormones in modulating phagocytic cell performance of dairy cattle under inflammatory conditions.

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Section: Neuroendocrine Responses To Stressmentioning

confidence: 99%

Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review

Alhussien

Dang

2020

Vet World

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“…Others have shown that the extracellular receptor kinase (ERK) and Janus kinase (JNK) pathways in membranes produce inflammation at term and preterm parturition [ 21 ]. Regardless of signaling pathways, NF-kB activation and an increase in proinflammatory mediators are the final effectors of parturition [ 22 , 23 , 24 , 25 ]. This is a natural and physiological response at term, but is pathologic and prematurely activated in preterm parturitions.…”

Section: Introductionmentioning

confidence: 99%

Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane Inflammation

Kammala

Sheller‐Miller

Radnaa

et al. 2020

IJMS

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The fetal inflammatory response, a key contributor of infection-associated preterm birth (PTB), is mediated by nuclear factor kappa B (NF-kB) activation. Na+/H+ exchanger regulatory factor-1 (NHERF1) is an adapter protein that can regulate intracellular signal transduction and thus influence NF-kB activation. Accordingly, NHERF1 has been reported to enhance proinflammatory cytokine release and amplify inflammation in a NF-kB-dependent fashion in different cell types. The objective of this study was to examine the role of NHERF1 in regulating fetal membrane inflammation during PTB. We evaluated the levels of NHERF1 in human fetal membranes from term labor (TL), term not in labor (TNIL), and PTB and in a CD1 mouse model of PTB induced by lipopolysaccharide (LPS). Additionally, primary cultures of fetal membrane cells were treated with LPS, and NHERF1 expression and cytokine production were evaluated. Gene silencing methods using small interfering RNA targeting NHERF1 were used to determine the functional relevance of NHERF1 in primary cultures. NHERF1 expression was significantly (p < 0.001) higher in TL and PTB membranes compared to TNIL membranes, and this coincided with enhanced (p < 0.01) interleukin (IL)-6 and IL-8 expression levels. LPS-treated animals delivering PTB had increased levels of NHERF1, IL-6, and IL-8 compared to phosphate-buffered saline (PBS; control) animals. Silencing of NHERF1 expression resulted in a significant reduction in NF-kB activation and IL-6 and IL-8 production as well as increased IL-10 production. In conclusion, downregulation of NHERF1 increased anti-inflammatory IL-10, and reducing NHERF1 expression could be a potential therapeutic strategy to reduce the risk of infection/inflammation associated with PTB.

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“…Lipopolysaccharide (LPS) was used to model inflammation as it stimulates inflammatory cells of the immune system . LPS is a pathogenic endotoxic component in the outer membrane of Gram‐negative bacteria and can disturb the balance between immunity and inflammatory response . LPS stimulation triggers production of proinflammatory cytokines such as tumor necrosis factor alpha (TNFα) and interleukin 6 (IL‐6), which mediate septic shock and acute lung inflammation .…”

Section: Introductionmentioning

confidence: 99%

Modulating Inflammation in Monocytes Using Capillary Fiber Organic Electronic Ion Pumps

Seitanidou

Blomgran

Pushpamithran

et al. 2019

Adv Healthcare Materials

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techniques. [5,6] This technology can serve as an efficient tool for an application such as biosensing, [7][8][9] electrophysiological recording, [10] and drug delivery. [11][12][13] Organic electronic ion pumps (OEIPs) are a primary example of organic bioelectronics combining electronic and ionic properties of organic electronics materials [14][15][16] to enable release, via electronic addressing, of ionic-biochemical signals for biological applications. OEIPs operate as an "iontronic" resistors [17][18][19] and can be used to electrophoretically deliver charged species through a cation or anion exchange membrane (AEM), resulting in high spatiotemporal delivery resolution and high dosage precision (one electron per delivered monovalent ion). [14] In recent years, these electrophoretic delivery devices have been used to trigger cell signaling in vitro, [1,20] to control epileptiform activity in brain slice models, [21][22][23] to effect sensory function in vivo, [19] to suppress pain sensation in awake animals, [17] and to modulate plant physiology. [24,25] OEIP devices based on glass capillary fibers offer several design advantages for use in freestanding or implantable geometries. OEIPs fiber capillaries, in contrast to planar OEIPs devices, provide less water uptake inside the channel providing a large ion-transport cross-section with high ionic conductivity, which allows transport of relatively large ions such as drugs and neurotransmitters. [26,27] These devices can furthermore be more easily implanted or located in proximity to targeted cells, tissues, or organs.In the present work, we demonstrate OEIPs based on glass capillary fibers that are filled with a polyelectrolyte (polycationic AEM). The AEM is characterized by a high concentration of fixed positive charges that allows for the selective transport of negative ions while blocking coions from drifting in the opposite direction. Nonfixed mobile ions with the same charge with the fixed charges of ion exchange membrane are referred to as coions while ions with opposite charge are referred to as counterions. The permselectivity, according to Donnan exclusion, holds if the ionic concentrations in the adjacent electrolytes are considerably lower than the fixed charge concentration of the AEM. [28] The potential gradient, and associated current through the OEIPs fiber capillaries, is established by applying a potential difference between electrodes (Ag/AgCl) applied in the source and target solutions. [27] The polarizable electrodes in this An organic electronic ion pump (OEIP) delivers ions and drugs from a source, through a charge selective membrane, to a target upon an electric bias. Miniaturization of this technology is crucial and will provide several advantages, ranging from better spatiotemporal control of delivery to reduced invasiveness for implanted OEIPs. To miniaturize OEIPs, new configurations have been developed based on glass capillary fibers filled with an anion exchange membrane (AEM). Fiber capillary OEIPs can be easily implanted in proximi...

show abstract

Cortisol modulates inflammatory responses in LPS-stimulated RAW264.7 cells via the NF-κB and MAPK pathways

Cited by 54 publications

References 37 publications

Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review

Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review

Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane Inflammation

Modulating Inflammation in Monocytes Using Capillary Fiber Organic Electronic Ion Pumps

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