Corto and DSP1 interact and bind to a maintenance element of the Scr Hox gene: understanding the role of Enhancers of trithorax and Polycomb

Salvaing, Juliette; Decoville, Martine; Mouchel-Vielh, Emmanuèle; Bussière, Marianne; Daulny, Anne; Boldyreva, Lidiya V.; Жимулев, И. Ф.; Locker, Daniel; Peronnet, Frédérique

doi:10.1186/1741-7007-4-9

Cited by 21 publications

(20 citation statements)

References 51 publications

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“…Dsp1 has been shown to coimmunoprecipitate with the chromo domain containing protein, Corto (Salvaing et al, 2006). These authors suggest that Dsp1 binds to the Scr PRE only when Scr is being transcribed, thus Dsp1 plays a critical role in TRE activity.…”

Section: Pres In Drosophilamentioning

confidence: 99%

Polycomb Group Response Elements in Drosophila and Vertebrates

Kassis¹,

Brown²

2013

Advances in Genetics

202

218

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Polycomb group genes (PcG) encode a group of about 16 proteins that were first identified in Drosophila as repressors of homeotic genes. PcG proteins are present in all metazoans and are best characterized as transcriptional repressors. In Drosophila, these proteins are known as epigenetic regulators because they remember, but do not establish, the patterned expression state of homeotic genes throughout development. PcG proteins, in general, are not DNA binding proteins, but act in protein complexes to repress transcription at specific target genes. How are PcG proteins recruited to the DNA? In Drosophila, there are specific regulatory DNA elements called Polycomb group response elements (PREs) that bring PcG protein complexes to the DNA. Drosophila PREs are made up of binding sites for a complex array of DNA binding proteins. Functional PRE assays in transgenes have shown that PREs act in the context of other regulatory DNA and PRE activity is highly dependent on genomic context. Drosophila PREs tend to regulate genes with a complex array of regulatory DNA in a cell or tissue-specific fashion and it is the interplay between regulatory DNA that dictates PRE function. In mammals, PcG proteins are more diverse and there are multiple ways to recruit PcG complexes, including RNA-mediated recruitment. In this review, we discuss evidence for PREs in vertebrates and explore similarities and differences between Drosophila and vertebrate PREs.

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Section: Pres In Drosophilamentioning

confidence: 99%

Polycomb Group Response Elements in Drosophila and Vertebrates

Kassis¹,

Brown²

2013

Advances in Genetics

202

218

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“…5-Bromo-2Ј-deoxyuridine (BrdU) labeling was for 45 min and standard protocols were followed thereafter (de Nooij et al, 1996). Immunostaining of polytene chromosomes was performed according to Salvaing et al (2003), by using mouse anti-DREF (1:50) and guinea pig anti-Pzg (1:1000). Secondary antibodies coupled to fluorescein, Cy3, or Cy5 were purchased from Jackson Laboratories.…”

Section: Antibody Stainings Of Tissues and Documentation Of Phenotypesmentioning

confidence: 99%

putzigIs Required for Cell Proliferation and Regulates Notch Activity inDrosophila

Kugler

Nagel

2007

MBoC

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We have identified the gene putzig (pzg) as a key regulator of cell proliferation and of Notch signaling in Drosophila. pzg encodes a Zn-finger protein that was found earlier within a macromolecular complex, including TATA-binding protein-related factor 2 (TRF2)/DNA replication-related element factor (DREF). This complex is involved in core promoter selection, where DREF functions as a transcriptional activator of replication-related genes. Here, we provide the first in vivo evidence that pzg is required for the expression of cell cycle and replication-related genes, and hence for normal developmental growth. Independent of its role in the TRF2/DREF complex, pzg acts as a positive regulator of Notch signaling that may occur by chromatin activation. Down-regulation of pzg activity inhibits Notch target gene activation, whereas Hedgehog (Hh) signal transduction and growth regulation are unaffected. Our findings uncover different modes of operation of pzg during imaginal development of Drosophila, and they provide a novel mechanism of Notch regulation.

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“…ETPs also comprise the evolutionary conserved GAGA factor that interacts with both BAP and PRC complexes in the regulation of Hox genes [ 35 ] and the proteins Corto and dorsal switch protein 1 (DSP1) in Drosophila [ 36 , 37 ]. Corto interacts physically with GAGA and DSP1 which are both involved in the recruitment of PcG complexes to Polycomb Response Elements (PRE) [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

Drosophila Cyclin G and epigenetic maintenance of gene expression during development

Dupont

Dardalhon-Cuménal

Kyba

et al. 2015

Epigenetics & Chromatin

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BackgroundCyclins and cyclin-dependent kinases (CDKs) are essential for cell cycle regulation and are functionally associated with proteins involved in epigenetic maintenance of transcriptional patterns in various developmental or cellular contexts. Epigenetic maintenance of transcription patterns, notably of Hox genes, requires the conserved Polycomb-group (PcG), Trithorax-group (TrxG), and Enhancer of Trithorax and Polycomb (ETP) proteins, particularly well studied in Drosophila. These proteins form large multimeric complexes that bind chromatin and appose or recognize histone post-translational modifications. PcG genes act as repressors, counteracted by trxG genes that maintain gene activation, while ETPs interact with both, behaving alternatively as repressors or activators. Drosophila Cyclin G negatively regulates cell growth and cell cycle progression, binds and co-localizes with the ETP Corto on chromatin, and participates with Corto in Abdominal-B Hox gene regulation. Here, we address further implications of Cyclin G in epigenetic maintenance of gene expression.ResultsWe show that Cyclin G physically interacts and extensively co-localizes on chromatin with the conserved ETP Additional sex combs (ASX), belonging to the repressive PR-DUB complex that participates in H2A deubiquitination and Hox gene silencing. Furthermore, Cyclin G mainly co-localizes with RNA polymerase II phosphorylated on serine 2 that is specific to productive transcription. CycG interacts with Asx, PcG, and trxG genes in Hox gene maintenance, and behaves as a PcG gene. These interactions correlate with modified ectopic Hox protein domains in imaginal discs, consistent with a role for Cyclin G in PcG-mediated Hox gene repression.ConclusionsWe show here that Drosophila CycG is a Polycomb-group gene enhancer, acting in epigenetic maintenance of the Hox genes Sex combs reduced (Scr) and Ultrabithorax (Ubx). However, our data suggest that Cyclin G acts alternatively as a transcriptional activator or repressor depending on the developmental stage, the tissue or the target gene. Interestingly, since Cyclin G interacts with several CDKs, Cyclin G binding to the ETPs ASX or Corto suggests that their activity could depend on Cyclin G-mediated phosphorylation. We discuss whether Cyclin G fine-tunes transcription by controlling H2A ubiquitination and transcriptional elongation via interaction with the ASX subunit of PR-DUB.Electronic supplementary materialThe online version of this article (doi:10.1186/s13072-015-0008-6) contains supplementary material, which is available to authorized users.

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Corto and DSP1 interact and bind to a maintenance element of the Scr Hox gene: understanding the role of Enhancers of trithorax and Polycomb

Cited by 21 publications

References 51 publications

Polycomb Group Response Elements in Drosophila and Vertebrates

Polycomb Group Response Elements in Drosophila and Vertebrates

putzigIs Required for Cell Proliferation and Regulates Notch Activity inDrosophila

Drosophila Cyclin G and epigenetic maintenance of gene expression during development

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