Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: fundamentals and applications

Liu, Xiuxia; Li, Ye; Bai, Zhonghu

doi:10.1016/b978-0-12-821477-0.00006-4

Cited by 9 publications

(6 citation statements)

References 300 publications

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“…No carbon catabolite repression, versatile Cmetabolism GRAS-status (Gopinath and Nampoothiri, 2014;Lin et al, 2022;Liu et al, 2021) Clostridium spp. Specialty metabolism: Solventogenic, natural polyketide production, versatile metabolism Versatile use of feedstock: CO 2 , H 2 , complex biomasses such as lignocellulose (Bosma et al, 2013;Cheng et al, 2019;Herman et al, 2017;Liberato et al, 2019) Bacillus subtilis Specialty metabolism: Allows high protein expression levels, industrially relevant metabolism Versatile use of feedstock: Cheap carbon sources GRAS-status (Gu et al, 2018;Su et al, 2020)…”

Section: Universal Gene Expression Machinerymentioning

confidence: 99%

Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools

Wannemaeker

Bervoets²,

Mey³

2022

Biotechnology Advances

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Section: Universal Gene Expression Machinerymentioning

confidence: 99%

Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools

Wannemaeker

Bervoets²,

Mey³

2022

Biotechnology Advances

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“…Later, the same group increased tryptophan production by fine-tuning of the TrpE fbr D, aroG fbr , and serA fb , yielding a titer of 1.31 mmol/l in flask culture [ 105 ]. VioE was found to be the rate-limiting enzyme and overexpression of VioE led to the highest production of violacein at 12.96 mmol/l without the exogenous addition of tryptophan [ 106 ]. Finally, the E. coli BL21 (DE3) with heterologous violacein pathway was screened in a high-throughput manner with the plasmid-based genome-scale sRNA library, which targets 1,858 E. coli genes.…”

Section: Natural Violacein Producersmentioning

confidence: 99%

Recent Advances in Synthetic, Industrial and Biological Applications of Violacein and Its Heterologous Production

Ahmed

Ahmad²,

et al. 2021

J. Microbiol. Biotechnol.

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Violacein, a purple pigment first isolated from a gram-negative coccobacillus Chromobacterium violaceum, has gained extensive research interest in recent years due to its huge potential in the pharmaceutic area and industry. In this review, we summarize the latest research advances concerning this pigment, which include (1) fundamental studies of its biosynthetic pathway, (2) production of violacein by native producers, apart from C. violaceum, (3) metabolic engineering for improved production in heterologous hosts such as Escherichia coli, Citrobacter freundii, Corynebacterium glutamicum, and Yarrowia lipolytica, (4) biological/pharmaceutical and industrial properties, (5) and applications in synthetic biology. Due to the intrinsic properties of violacein and the intermediates during its biosynthesis, the prospective research has huge potential to move this pigment into real clinical and industrial applications.

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“…Although protease-reduced strains exist (Murashima et al 2002;Nguyen et al 2011;Wu et al 1991), other hosts represent promising alternatives, among them the diderm Gram-positive Corynebacterium glutamicum, which exhibits an intrinsically low extracellular proteolytic activity (Vertès 2013). It is long time used in the industry for the large-scale production of amino acids such as l-glutamate and l-lysine (Hermann 2003;Leuchtenberger et al 2005), as well as for the production of other small molecules like l-ornithine as summarized by Liu et al (2021). Recently however, C. glutamicum also became of interest for recombinant protein production and signal peptide-guided protein secretion via the Sec and Tat pathway (Freudl 2017;Lee and Kim 2018).…”

Section: Introductionmentioning

confidence: 99%

Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation

Müller

Bakkes

Lenz

et al. 2022

Appl Microbiol Biotechnol

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Secretion of bacterial proteins into the culture medium simplifies downstream processing by avoiding cell disruption for target protein purification. However, a suitable signal peptide for efficient secretion needs to be identified, and currently, there are no tools available to predict optimal combinations of signal peptides and target proteins. The selection of such a combination is influenced by several factors, including protein biosynthesis efficiency and cultivation conditions, which both can have a significant impact on secretion performance. As a result, a large number of combinations must be tested. Therefore, we have developed automated workflows allowing for targeted strain construction and secretion screening using two platforms. Key advantages of this experimental setup include lowered hands-on time and increased throughput. In this study, the automated workflows were established for the heterologous production of Fusarium solani f. sp. pisi cutinase in Corynebacterium glutamicum. The target protein was monitored in culture supernatants via enzymatic activity and split GFP assay. Varying spacer lengths between the Shine-Dalgarno sequence and the start codon of Bacillus subtilis signal peptides were tested. Consistent with previous work on the secretory cutinase production in B. subtilis, a ribosome binding site with extended spacer length to up to 12 nt, which likely slows down translation initiation, does not necessarily lead to poorer cutinase secretion by C. glutamicum. The best performing signal peptides for cutinase secretion with a standard spacer length were identified in a signal peptide screening. Additional insights into the secretion process were gained by monitoring secretion stress using the C. glutamicum K9 biosensor strain. Key points • Automated workflows for strain construction and screening of protein secretion • Comparison of spacer, signal peptide, and host combinations for cutinase secretion • Signal peptide screening for secretion by C. glutamicum using the split GFP assay

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Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: fundamentals and applications

Cited by 9 publications

References 300 publications

Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools

Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools

Recent Advances in Synthetic, Industrial and Biological Applications of Violacein and Its Heterologous Production

Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation

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