Cosmid-derived markers anchoring the bovine genetic map to the physical map

Ferretti, Luca; Urquhart, B G D; Eggen, Andre A.; Olsaker, I.; Harlizius, B.; Castiglioni, Bianca; Mezzelani, Alessandra; Toldo, S. Solinas; Thieven, U.; Zhang, Y.; Morgan, A. L. G.; Teres, V. M.; Schwerin, Manfred; Martín-Burriel, Inmaculada; Chowdhary, Bhanu P.; Erhardt, G.; Nijman, Isaäc J; Cribiu, Edmond; Barendse, W.; Levéziel, Hubert; Fries, R.; Williams, J. L.

doi:10.1007/s003359900341

Cited by 27 publications

(11 citation statements)

References 52 publications

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“…Cosmid clone JAB8 (cJAB8; Ferretti et al, 1997) was used as a cattle chromosome 7 (BTA7)-specific DNA probe, since it produces a strong FISH signal at the sub-centromeric region of BTA7. The application of this probe, however, required suppression hybridisation of repetitive sequences, which precluded its combinatorial use with pericentromeric or telomeric DNA probes.…”

Section: Dna Probes and Labelingmentioning

confidence: 99%

Centromere and telomere redistribution precedes homologue pairing and terminal synapsis initiation during prophase I of cattle spermatogenesis

Pfeifer¹,

Thomsen²,

Scherthan

2001

Cytogenet Genome Res

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Alterations in nuclear topology associated with meiotic chromosome pairing were studied in premeiotic cells and spermatocytes I of adult bovine males. To this end, we performed FISH with chromosome, pericentromeric satellite-DNA and telomere-specific probes in combination with immunostaining of synaptonemal complex proteins (SCP3, SCP1) on testis tissue sections. Nuclei of premeiotic cells (spermatogonia) exhibited a scattered telomere distribution while pericentromeres formed a few intranuclear clusters. We observed that the chromosome pairing process in cattle prophase I is preceded by repositioning of centromeres and telomeres to the nuclear periphery during preleptotene. Clustering of chromosome ends (bouquet formation) was observed during the transition from leptonema to zygonema and coincided with pairing of a sub-centromeric marker of bovine chromosomes 7. Dissolution of bouquet topology during zygonema left perinuclear telomeres scattered over the nuclear periphery at pachynema. SCP3 staining in frozen tissue sections revealed the appearance of this axial element protein in intranuclear aggregates during preleptotene, followed by extensive axial element formation during leptotene. Synapsis as revealed by SCP1 staining initiated peripherally at earliest zygotene, at this stage nuclei still contained numerous SCP3 clusters. Our observations reveal prominent non-homologous satellite-DNA associations in spermatogonia and indicate the conservation of topological features of the meiotic chromosome pairing process among mammals. The comparison of telomere dynamics in mouse and cattle prophase I suggests that a larger number of chromosomes prolongs the duration of the bouquet stage.

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Section: Dna Probes and Labelingmentioning

confidence: 99%

Centromere and telomere redistribution precedes homologue pairing and terminal synapsis initiation during prophase I of cattle spermatogenesis

Pfeifer¹,

Thomsen²,

Scherthan

2001

Cytogenet Genome Res

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“…'n the TEXAS NoMENCLATURE (1996) and both river buffalo stand" ,.J ' 'ryot · ~ (CsKBB 1994) and recent FISH-mapping data (IAN-NUZZI et at !d) which followed the IscNDA89 (1990) system. EGGEN andFRIEs (1995) andFERRETTI et al (1997).…”

Section: Resultsmentioning

confidence: 99%

A genetic physical map in river buffalo (Bubalus bubalis, 2n=50)

Iannuzzi¹

1998

Caryologia

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SUMMARY-This study reports the first entire genetic physical map in river buffalo chromosomes by ISH-techniques. Thirty-six loci (mostly mapped by FISH), of which 19 were expressed genes and 17 were DNA-segments (cosmid derived-microsatellites), were localized in specific chromosomes. At least one molecular marker from all 31 bovine syntenic groups was assigned to each river buffalo chromosome. This allowed tentative assignment of all bovine syntetic groups to river buffalo chromosomes, also in consideration of high degree of chromosome banding homologies between the two species and that all mapped loci in river buffalo chromosomes were at the same cattle homoeologous chromosomes and chromosome bands. By adding markers from bovine U1, U3, U7, UlO, U13, U16, U17, U25, U28 and U29 assigned to the river buffalo genome by the somatic cell hybrid technique, a total of 54 markers is assigned to river buffalo genome.

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“…All loci mapped on homoeologous chromosomes and chromosome regions in the two species (Table 1), confirming the high degree of similarity between the autosomes of bovids (Hayes et al, 1991;Gallagher and Womack et al, 1992;Iannuzzi and Di Meo, 1995), and further supporting the hypothesis that banding pattern similarities indicate conservation of genome organization. Small discrepancies in the localization of some loci on different chromosome bands of sheep, river buffalo and cattle may result from technical problems such as different banding techniques applied in cattle (Q-banding, Mezzelani et al, 1995;Ferretti et al, 1997) and in the present study (R-banding), as well as from different banded ideograms adopted in cattle (Mezzelani et al, 1995;Ferretti et al, 1997), in sheep (ISCNDB 2000) and in river buffalo (CSKBB 1994).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, 28 bovine cosmids from 19 bovine syntenic groups (U), three of which contain type I loci and 25 which contain microsatellites, previously assigned to cattle chromosomes (Mezzelani et al, 1995;Ferretti et al 1997), were com- paratively FISH-mapped to sheep and river buffalo R-banded chromosomes (13 loci for the first time), thus significantly extending the physical maps of these two species.…”

mentioning

confidence: 99%

Comparative mapping of twenty-eight bovine loci in sheep (<i>Ovis aries</i>, 2n = 54) and river buffalo (<i>Bubalus bubalis</i>, 2n = 50) by FISH

Meo

Perucatti²,

Incarnato³

et al. 2002

Cytogenet Genome Res

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Peripheral blood cell cultures were treated for late incorporation of both BrdU and Hoechst-33258 to obtain R-banding pattern preparations. Twenty-eight bovine cosmids from 19 bovine syntenic groups (U), three of which contain type I loci and 25 which contain microsatellite loci and have previously been assigned to cattle chromosomes, were comparatively FISH-mapped to sheep and river buffalo chromosomes according to the standard karyotypes (13 loci for the first time in the latter species). The results enrich the physical maps of both species with information relative to the following loci and to the corresponding syntenic groups: IDVGA35 and IDVGA53 (U6), IDVGA61 and IDVGA84 (U13), JAB10 (U5), IDVGA41 and IDVGA57 (U27), IDVGA87 (U11), IDVGA32 and IDVGA10 (U19), IDVGA49, IDVGA66 and IDVGA68 (U1), ZNF164 (U23), IDVGA74 and IDVGA70 (U9), IDVGA47, IDVGA46 and IDVGA58 (U21), MAP1B (U14), IDVGA79 (U4), CATHL (U12), IDVGA71 (U8), IDVGA59 (U26), IDVGA29 (U29), IDVGA7 (U7), IDVGA82 (X), IDVGA50 (Y). All mapped loci were localized on homoeologous chromosomes and chromosome regions of the two species, confirming the high degree of chromosome homoeologies between the subfamilies Bovinae and Caprinae.

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Cosmid-derived markers anchoring the bovine genetic map to the physical map

Abstract: Abstract. The mapping strategy for the bovine genome described

Cited by 27 publications

References 52 publications

Centromere and telomere redistribution precedes homologue pairing and terminal synapsis initiation during prophase I of cattle spermatogenesis

Centromere and telomere redistribution precedes homologue pairing and terminal synapsis initiation during prophase I of cattle spermatogenesis

A genetic physical map in river buffalo (Bubalus bubalis, 2n=50)

Comparative mapping of twenty-eight bovine loci in sheep (<i>Ovis aries</i>, 2n = 54) and river buffalo (<i>Bubalus bubalis</i>, 2n = 50) by FISH

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