Cost Effective Paper-Based Colorimetric Microfluidic Devices and Mobile Phone Camera Readers for the Classroom

Koesdjojo, Myra T.; Pengpumkiat, Sumate; Wu, Yuanyuan; Boonloed, Anukul; Huynh, Danny; Remcho, Thomas P.; Remcho, Vincent T.

doi:10.1021/ed500401d

“…Ford etection by naked eye,w eu sed SYBR green dye [38] and ab lue LED flashlight to get an explicit fluorescence signal when virus RNAi sp resent. [39] ZIKV canb ed etectedi nb lood,s aliva, urine,a nd semen, rangingfromdaystomonthsafter onsetofsymptoms. Thet est result was further captured using as martphone camera and confirmed using gel electrophoresis.I ti sc onceivable to develop as martphone app to extract semiquantitative results from these color pictures as demonstrated in the literature.…”

Section: Angewandte Chemiementioning

confidence: 99%

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

Jiang

¹

,

Loeb

²

,

Manzanas

³

et al. 2018

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The recent outbreaks of Zika virus (ZIKV) infection represent ap ublic health challenge.R apid, cost-effective,a nd reliable diagnostic tools for ZIKV detection at the point of care (POC) are highly desirable,e specially for resource-limited nations.T oaddress the need, we have developed an integrated device to achieve sample-to-answer ZIKV detection. The device features innovative ball-based valves enabling the storage and sequential delivery of reagents for virus lysis and apaper-based unit for RNAenrichmentand purification. The paper unit is placed in ac ommercially available coffee mug that provides ac onstant temperature for reverse transcription loop-mediated isothermal amplification (RT-LAMP), followed by colorimetric detection by naked eye or ac ellphone camera. Using the device,w ed emonstrated the reproducible detection of ZIKV in human urine and saliva samples.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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“…Further incorporation of microfluidic devices to science education in combination with smartphones is not far from becoming a reality. Toward this direction, MS 2 is anticipated to become a common science educational tool at all levels of schools and institutions in the near future 108 .…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Novel developments in mobile sensing based on the integration of microfluidic devices and smartphones

Yang

¹

,

Peretz‐Soroka

²

,

Liu

³

et al. 2016

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Portable electronic devices and wireless communication systems enable a broad range of applications such as environmental and food safety monitoring, personalized medicine and healthcare management. Particularly, hybrid smartphone and microfluidic devices provide an integrated solution for the new generation of mobile sensing applications. Such mobile sensing based on microfluidic devices (broadly defined) and smartphones (MS(2)) offers a mobile laboratory for performing a wide range of bio-chemical detection and analysis functions such as water and food quality analysis, routine health tests and disease diagnosis. MS(2) offers significant advantages over traditional platforms in terms of test speed and control, low cost, mobility, ease-of-operation and data management. These improvements put MS(2) in a promising position in the fields of interdisciplinary basic and applied research. In particular, MS(2) enables applications to remote in-field testing, homecare, and healthcare in low-resource areas. The marriage of smartphones and microfluidic devices offers a powerful on-chip operating platform to enable various bio-chemical tests, remote sensing, data analysis and management in a mobile fashion. The implications of such integration are beyond telecommunication and microfluidic-related research and technology development. In this review, we will first provide the general background of microfluidic-based sensing, smartphone-based sensing, and their integration. Then, we will focus on several key application areas of MS(2) by systematically reviewing the important literature in each area. We will conclude by discussing our perspectives on the opportunities, issues and future directions of this emerging novel field.

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“…The test result was further captured using a smartphone camera and confirmed using gel electrophoresis. It is conceivable to develop a smartphone app to extract semi‐quantitative results from these color pictures as demonstrated in the literature …”

Section: Figurementioning

confidence: 99%

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

Jiang

¹

,

Loeb

²

,

Manzanas

³

et al. 2018

View full text Add to dashboard Cite

The recent outbreaks of Zika virus (ZIKV) infection represent ap ublic health challenge.R apid, cost-effective,a nd reliable diagnostic tools for ZIKV detection at the point of care (POC) are highly desirable,e specially for resource-limited nations.T oaddress the need, we have developed an integrated device to achieve sample-to-answer ZIKV detection. The device features innovative ball-based valves enabling the storage and sequential delivery of reagents for virus lysis and apaper-based unit for RNAenrichmentand purification. The paper unit is placed in ac ommercially available coffee mug that provides ac onstant temperature for reverse transcription loop-mediated isothermal amplification (RT-LAMP), followed by colorimetric detection by naked eye or ac ellphone camera. Using the device,w ed emonstrated the reproducible detection of ZIKV in human urine and saliva samples.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

show abstract

Cost Effective Paper-Based Colorimetric Microfluidic Devices and Mobile Phone Camera Readers for the Classroom

Cited by 93 publications

References 17 publications

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

Novel developments in mobile sensing based on the integration of microfluidic devices and smartphones

Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection

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