“…The single-cell (comet) gel assay is an excellent, reliable method for evaluating DNA strand breakage, including DNA adducts, single-and double-strand breaks, and deficient repair sites. This technique is a simple method that allows the proper investigation of DNA strand breaks that can originate from many contexts and paradigms 16 . In this review, the comet assay was the preferred method for evaluating genetic damage by sleep deprivation as the majority of papers (three studies) have demonstrated positive genotoxicity induced by sleep deprivation in multiple organs of rodents by comet assay.…”