Coulometric High-Performance Liquid Chromatographic Analysis of Morphine in Biological Fluids

Simultaneous Measurement of Extracellular Morphine and Serotonin in Brain Tissue and CSF by Microdialysis in Awake Rats

“…In a recent review, Tagliaro et al (1989) concluded that separation by reversed-phase HPLC with ECD, because of its specificity, reliability, and sensitivity, is the method of choice for assaying morphine. A coulometric three-electrode arrangement is frequently used because it allows the removal of interfering substances from mobile phase and sample, by selective oxidation at the first two electrodes ( h n d o r f and Kaltenbach, 1986;Svensson, 1986;Kouishi and Hashimoto, 1990;McLean et al, 1990). However, because 5-HT and 5-HIAA are easily oxidized at low potentials at the first electrode and no longer detected at the analytical cell, we in fact could not fully exploit the advantages of the coulometric tluee&xtmde array in our assay.…”

Tribute to Professor Hartmut Derendorf ‐ 1953 to 2020: Driving force in Clinical Pharmacology and Mentor Extraordinaire

“…In the early 1980s, Derendorf et al measured the drug concentrations in saliva as a proxy of free tissue concentrations and analgesic efficacy 1 before expanding their work to other target phases, such as joints, 2 the brain, 3 and various biological fluids. 4 His group also became interested in local treatment strategies Schreier. [5][6][7] A technological breakthrough was achieved in Hartmut's laboratory in the mid 1990s 8 by adopting the microdialysis technique, as it provided access to unbound interstitial space fluid concentrations.…”

“…In the early 1980s, Derendorf et al . measured the drug concentrations in saliva as a proxy of free tissue concentrations and analgesic efficacy 1 before expanding their work to other target phases, such as joints, 2 the brain, 3 and various biological fluids 4 . His group also became interested in local treatment strategies Schreier 5–7 …”

Improved method for morphine determination in biological fluids and tissues: rapid, sensitive and selective

“…Correspondence to: C. F. McLean, Department of Anaesthesia and Intensive Care, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia. upon gas-liquid chromatography following derivatization (Dahlstrom et al 1977;Felby 1979;Jones et al 1984), reverse phase HPLC following solid phase or solvent extraction with electrochemical (Bolander et al 1983;Moore et a1 1984;Derendorf & Kaltenbach 1986;Svensson 1986), UV-VIS (Svensson et al 1982;Sawe et al 1985) or fluorescence detection (Nelson et al 1982;Tagliaro et a1 1985), or radioimmunoassay (RIA) methodology (Morris 1975;Hahn et a1 1979;Stanski et al 1982;Edwards et a1 1986). Most of these methods require several timeconsuming sample preparation steps before quantitation.…”