Coumarin-Conjugated Macromolecular Probe for Sequential Stimuli-Mediated Activation
Yu Jiang,
Runqi Li,
Fei Ren
et al.
Abstract:Protein bioconjugation has emerged as one of the most valuable tools for the development of protein-based biochemical assays. Here, we report a fluorescent macromolecular material, RF16_Halo, in which the coumarin derivative RF16 is specifically conjugated onto HaloTag protein to achieve a dual-stimuli-mediated fluorescence response. RF16 is first obtained by installing a H 2 O 2 -sensitive boron cage onto the C7 hydroxy moiety of the coumarin fluorophore with a HaloTag ligand attaching to the pH-labile 1,3dio… Show more
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