Counterflow Isotachophoresis As a Method of Concentration and Isolation of DNA from Biological Fluids

Кондратова, В. Н.; Serdyuk, O. I.; Шелепов, В. П.; Потапова, Г. И.; Лихтенштейн, А. В.

doi:10.1007/s10628-005-0070-2

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…The studies of Kondratova et al 383 and Kondratova et al 92 in 2005 were the earliest to report the extraction of DNA from biological samples such as plasma and serum using ITP. They demonstrated their method using fairly standard laboratory equipment (not microfluidics) and agarose gels as a downstream step following a series of purification steps.…”

Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

Isotachophoresis: Theory and Microfluidic Applications

Ramachandran

Santiago

2022

Chem. Rev.

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Isotachophoresis (ITP) is a versatile electrophoretic technique that can be used for sample preconcentration, separation, purification, and mixing, and to control and accelerate chemical reactions. Although the basic technique is nearly a century old and widely used, there is a persistent need for an easily approachable, succinct, and rigorous review of ITP theory and analysis. This is important because the interest and adoption of the technique has grown over the last two decades, especially with its implementation in microfluidics and integration with on-chip chemical and biochemical assays. We here provide a review of ITP theory starting from physicochemical first-principles, including conservation of species, conservation of current, approximation of charge neutrality, pH equilibrium of weak electrolytes, and so-called regulating functions that govern transport dynamics, with a strong emphasis on steady and unsteady transport. We combine these generally applicable (to all types of ITP) theoretical discussions with applications of ITP in the field of microfluidic systems, particularly on-chip biochemical analyses. Our discussion includes principles that govern the ITP focusing of weak and strong electrolytes; ITP dynamics in peak and plateau modes; a review of simulation tools, experimental tools, and detection methods; applications of ITP for on-chip separations and trace analyte manipulation; and design considerations and challenges for microfluidic ITP systems. We conclude with remarks on possible future research directions. The intent of this review is to help make ITP analysis and design principles more accessible to the scientific and engineering communities and to provide a rigorous basis for the increased adoption of ITP in microfluidics.

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Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

Isotachophoresis: Theory and Microfluidic Applications

Ramachandran

Santiago

2022

Chem. Rev.

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“…In 1975 Brocek et al [ 83 ] demonstrated the first on-chip ITP separation of six carboxylic acids, and ITP extraction of nucleic acid extraction was first demonstrated by a group of cancer researchers in Moscow [ 84 , 85 ]. ITP has been integrated with a variety of lysis and amplification methods and applied to various types of pathogens.…”

Section: Nucleic Acid Extractionmentioning

confidence: 99%

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Basha

Yousuff

et al. 2017

Micromachines

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Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.

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“…However, the application of counterflow in an open capillary will inevitably introduce significant analyte dispersion due to the parabolic‐flow profile . To reduce dispersion in counterflow ITP, EOF has been used as counterflow to form a stationary ITP zone in porous media, such as polyacrylamide and agarose gel slabs . Breadmore et al also used EOF as counterflow to generate a stationary ITP stack followed by in‐line CZE separation, resulting in a detection limit 10 000 times lower than that using the normal hydrodynamic injection alone for CZE.…”

Section: Introductionmentioning

confidence: 99%

Counterflow isotachophoresis in a monolithic column

Liu

Cong

Ivory

2014

J of Separation Science

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This study describes stationary counterflow isotachophoresis (ITP) in a poly(acrylamide-co-N,N'-methylenebisacrylamide) monolithic column as a means for improving ITP processing capacity and reducing dispersion. The flow profile in the monolith was predicted using COMSOL's Brinkman Equation application mode, which revealed that the flow profile was mainly determined by monolith permeability. As monolith permeability decreases, the flow profile changes from a parabolic shape to a plug shape. An experimental monolithic column was prepared in a fused-silica capillary using an ultraviolet-initiated polymerization method. A monolithic column made from 8% (wt.) monomer was chosen for the stationary counterflow ITP experiments. Counterflow ITP in the monolithic column showed undistorted analyte zones with significantly reduced dispersion compared to the severe dispersion observed in an open capillary. Particularly, for r-phycoerythrin focused by counterflow ITP, its zone width in the monolithic column was only one-third that observed in an open capillary. These experiments demonstrate that stationary counterflow ITP in monoliths can be a robust and practical electrofocusing method.

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Counterflow Isotachophoresis As a Method of Concentration and Isolation of DNA from Biological Fluids

Cited by 6 publications

References 11 publications

Isotachophoresis: Theory and Microfluidic Applications

Isotachophoresis: Theory and Microfluidic Applications

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Counterflow isotachophoresis in a monolithic column

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