2012
DOI: 10.1016/j.ab.2012.02.012
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Coupling flash liquid chromatography with mass spectrometry for enrichment and isolation of milk oligosaccharides for functional studies

Abstract: Mass spectrometry has been coupled with flash liquid chromatography to yield new capabilities for isolating non-chromophoric material from complicated biological mixtures. A flash LC/MS/MS method enabled fraction collection of milk oligosaccharides from biological mixtures based on composition and structure. The method is compatible with traditional gas-pressure driven flow flash chromatography, widely employed in organic chemistry laboratories. The on-line mass detector enabled real-time optimization of chrom… Show more

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Cited by 16 publications
(14 citation statements)
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“…The chip’s highly reproducible retention times allowed assignment of several chemical structures ( e.g ., 3′- and 6′-sialyl-lactose) based on identical retention times or accurate mass; these assignments were based on commercial OS standards and data from earlier publications on milk OS (Table 1) (Strum et al, 2012; Wu et al, 2011, 2010). In addition to the structures reported in the literature, 29 GMO were identified by MS/MS, 11 of which were novel as indicated in the footnote at Table 1.…”
Section: Resultsmentioning
confidence: 99%
“…The chip’s highly reproducible retention times allowed assignment of several chemical structures ( e.g ., 3′- and 6′-sialyl-lactose) based on identical retention times or accurate mass; these assignments were based on commercial OS standards and data from earlier publications on milk OS (Table 1) (Strum et al, 2012; Wu et al, 2011, 2010). In addition to the structures reported in the literature, 29 GMO were identified by MS/MS, 11 of which were novel as indicated in the footnote at Table 1.…”
Section: Resultsmentioning
confidence: 99%
“…1,167 A number of methods for the development of SL and sialylated oligosaccharides have been studied, among which are isolation and microbial and enzymatic methods. [167][168][169] As soon as larger amounts of sialylated oligosaccharides become available, human studies can be started to confirm whether SL and sialylated oligosaccharides indeed contribute to the various health aspects described in this review. With the availability of larger amounts of these structures, supplementation of infant formula can become feasible as well.…”
Section: Application Of Oligosaccharides To Fortify Infant Formulasmentioning
confidence: 99%
“…Because of the important biological functions of SA, and considering that most of the effects of HMOs are highly structure specific, routes are being explored to develop HMO structures, such as SL and other sialylated oligosaccharides, for application in infant formula . A number of methods for the development of SL and sialylated oligosaccharides have been studied, among which are isolation and microbial and enzymatic methods . As soon as larger amounts of sialylated oligosaccharides become available, human studies can be started to confirm whether SL and sialylated oligosaccharides indeed contribute to the various health aspects described in this review.…”
Section: Application Of Oligosaccharides To Fortify Infant Formulasmentioning
confidence: 99%
“…To overcome these problems, different approaches have been proposed, such as the development of rapid methods using different materials, for example Superdex-type columns (GE Healthcare) based on highly cross-linked porous agarose beds to which dextran has been covalently bonded (Knutsen et al 2001), or the use of flash liquid chromatography (Flash LC), which uses glass columns and gas pressure-driven flow between 0 and 2 bar (Strum et al 2012). To overcome these problems, different approaches have been proposed, such as the development of rapid methods using different materials, for example Superdex-type columns (GE Healthcare) based on highly cross-linked porous agarose beds to which dextran has been covalently bonded (Knutsen et al 2001), or the use of flash liquid chromatography (Flash LC), which uses glass columns and gas pressure-driven flow between 0 and 2 bar (Strum et al 2012).…”
Section: Size-exclusion Chromatography (Sec)mentioning
confidence: 99%
“…This treatment was revealed to be a rapid method to obtain considerable amounts of GOS at gram scale. More recently, GCC Flash LC combined with real-time mass spectrometry detection has shown great potential to enrich and separate milligram quantities of specific human and bovine milk oligosaccharides, as well as to eliminate abundant saccharide components in milk, such as lactose and lacto-N-tetraose, and fractionate isomeric forms for more detailed biological assays (Strum et al 2012). This was the case reported by Hernández et al (2009) who obtained GOS of high purity with no presence of monosaccharides and lactose with 10% of ethanol, although the recovery of trisaccharides was very low.…”
Section: Activated Charcoalmentioning
confidence: 99%