Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations

Konášová, Renáta; Butnariu, Maria; Šolínová, Veronika; Kašička, Václav; Koval, Dušan

doi:10.1016/j.aca.2021.338789

Cited by 26 publications

(14 citation statements)

References 35 publications

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“…The mean value of EOF mobility was m eof = 24.3 ± 0.3 × 10 −9 m 2 V −1 s −1 (standard deviation [SD]) and relative SD (RSD) = 1.1% for n = 225 runs. For comparison, the inter‐batch repeatability of EOF in cationic 20% PAMAPTAC copolymer coatings was similar with RSD = 0.48% for n = 180 runs [18]. This underscore a solid performance of the coating procedure, in which framework is similar for either anionic PAMAMPS or cationic PAMAPTAC surface modifications.…”

Section: Resultsmentioning

confidence: 90%

“…EOF in sulfonate‐based PAMAMPS capillaries is virtually independent of pH of the BGE in the ∼2–10 interval, see Figure 3A. For comparison, similar plot for cationic PAMAPTAC coatings was more scattered than Figure 3A but still acceptable [18]. Typically, EOF rate drops with increasing ionic strength, which is documented in Figure 3B or S1.…”

Section: Resultsmentioning

confidence: 91%

“…The anionic PAMAMPS coatings are a complement to cationic PAMAPTAC coatings [18], which were so far used in several applications in clinical analysis [11][12][13][14].…”

Section: Discussionmentioning

confidence: 99%

“…In order to customize the EOF level for particular separation problem, we reported recently a system of covalent polymer coatings using cationic acrylamide (AM)‐based copolymer PAMAPTAC [18]. This coating strategy allows for reversed (i.e., anodic) EOF with a mobility spanning the 0–40 × 10 −9 m 2 V −1 s −1 interval that exhibits low pH dependence in the 2–10 pH range.…”

Section: Introductionmentioning

confidence: 99%

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Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

et al. 2022

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We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2‐acrylamido‐2‐methyl‐1‐propanesulfonate (AMPS), that is, poly(AM‐co‐AMPS) or PAMAMPS. Coatings were formed by the in‐capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to ∼40 × 10−9 m2 V−1 s−1 interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS‐treated capillaries, we observed (i) a negligible dependence on pH in the 2–10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter‐EOF mode.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 91%

“…The anionic PAMAMPS coatings are a complement to cationic PAMAPTAC coatings [18], which were so far used in several applications in clinical analysis [11][12][13][14].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

et al. 2022

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“…However, the use of non-uniform charge density along the capillary surface is generating peak broadening due to Taylor dispersion. [71] Another approach for the modulation of the EOF is to use an homogeneous coating of controlled charged density, [72,73] as exemplified in the case of peptide separations for a (PDADMAC/PAMAMPS) 1 in phosphate buffer at pH 2.5. The charge density was controlled by the molar content in AMPS in the PAMAMPS statistical copolymer.…”

Section: Using Smil Coatings For Cec Separationsmentioning

confidence: 99%

Polyelectrolyte Multilayers in Capillary Electrophoresis

et al. 2022

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Capillary electrophoresis (CE) has been proven to be a performant analytical method to analyze both small and macro molecules. Indeed, it is capable of separating compounds of the same nature according to differences in their charge to size ratios, particularly proteins, monoclonal antibodies and peptides. However, one of the major obstacles to reach high separation efficiency remains the adsorption of solutes on the capillary wall. Among the different coating approaches used to control and minimize solute adsorption, polyelectrolyte multi-layers can be applied to CE as a versatile approach. These coatings are made up of alternating layers of polycations and polyanions, and may be used in acidic, neutral or basic conditions depending on the solutes to be analyzed. This Review provides an overview of Successive Multiple Ionicpolymer Layer (SMIL) coatings used in CE, looking at how different parameters induce variations on the electro-osmotic flow (EOF), separation efficiency and coating stability, as well as their promising applications in the biopharmaceutical field.

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CE–MSApproaches for Peptidomics

Štěpánová¹,

Kašička²

2022

Capillary Electrophoresis‐Mass Spectrometry for Proteomics and Metabolomics

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Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations

Cited by 26 publications

References 35 publications

Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

Polyelectrolyte Multilayers in Capillary Electrophoresis

CE–MSApproaches for Peptidomics

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