Covalent functionalization of decellularized tissues accelerates endothelialization

Sasso, Eleonora Dal; Zamuner, Annj; Filippi, Andrea Riccardo; Romanato, Filippo; Palmosi, Tiziana; Vedovelli, Luca; Gregori, Darío; Ribelles, J.L. Gómez; Russo, Teresa; Gloria, Antonio; Iop, Laura; Gerosa, Gino; Dettin, Monica

doi:10.1016/j.bioactmat.2021.04.003

Cited by 14 publications

(8 citation statements)

References 75 publications

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“…In particular, the proposed surface modification chemistry follows simple protocols which fully avoid the use of organic solvents such as DMF and the strong acids often needed in this type of immobilization [14,16]. In turn, our anchoring strategy is more versatile and could be applied to biological matrices as well [36]. For surface functionalization, we employed aqueous solutions of peptides containing an aldehyde at the C-terminus.…”

Section: Discussionmentioning

confidence: 99%

Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces

et al. 2022

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In orthopedic, dental, and maxillofacial fields, joint prostheses, plates, and screws are widely used in the treatment of problems related to bone tissue. However, the use of these prosthetic systems is not free from complications: the fibrotic encapsulation of endosseous implants often prevents optimal integration of the prostheses with the surrounding bone. To overcome these issues, biomimetic titanium implants have been developed where synthetic peptides have been selectively grafted on titanium surfaces via Schiff base formation. We used the retro-inverted sequence (DHVPX) from [351–359] human Vitronectin and its dimer (D2HVP). Both protease-resistant peptides showed increased human osteoblast adhesion and proliferation, an augmented number of focal adhesions, and cellular spreading with respect to the control. D2HVP-grafted samples significantly enhance Secreted Phosphoprotein 1, Integrin Binding Sialoprotein, and Vitronectin gene expression vs. control. An estimation of peptide surface density was determined by Two-photon microscopy analysis on a silanized glass model surface labeled with a fluorescent analog.

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Section: Discussionmentioning

confidence: 99%

Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces

et al. 2022

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“…The surface containing RGD and SVVYGLR or YIGSR peptides can significantly enhance EC behaviors such as adhesion and migration owing to the synergistic effects of both peptides. 39 This dual-peptide modification method is beneficial for promoting the endothelialization of vascular grafts.…”

Section: Peptides For Enhancing Ec Adhesion Proliferation and Migrationmentioning

confidence: 99%

Recent strategies for improving hemocompatibility and endothelialization of cardiovascular devices and inhibition of intimal hyperplasia

et al. 2022

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“…In this respect, bioreactors and the cellularization procedures, which are used to simulate the native niche as much as possible regarding better endothelialization, are other essential factors in the endothelialization of acellular scaffolds ( Gorman et al, 2018 ). Additionally, surface biofunctionalization ( Dal Sasso et al, 2021 ; Keshi et al, 2021 ) and utilized cells play a crucial role in regulating the cells’ behavior and ultimately increasing the ECs coverage of the acellular vasculature.…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

Mazloomnejad

Babajani

Kasravi

et al. 2023

Front. Bioeng. Biotechnol.

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Decellularization of tissues and organs has recently become a promising approach in tissue engineering and regenerative medicine to circumvent the challenges of organ donation and complications of transplantations. However, one main obstacle to reaching this goal is acellular vasculature angiogenesis and endothelialization. Achieving an intact and functional vascular structure as a vital pathway for supplying oxygen and nutrients remains the decisive challenge in the decellularization/re-endothelialization procedure. In order to better understand and overcome this issue, complete and appropriate knowledge of endothelialization and its determining variables is required. Decellularization methods and their effectiveness, biological and mechanical characteristics of acellular scaffolds, artificial and biological bioreactors, and their possible applications, extracellular matrix surface modification, and different types of utilized cells are factors affecting endothelialization consequences. This review focuses on the characteristics of endothelialization and how to optimize them, as well as discussing recent developments in the process of re-endothelialization.

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Covalent functionalization of decellularized tissues accelerates endothelialization

Cited by 14 publications

References 75 publications

Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces

Selective Grafting of Protease-Resistant Adhesive Peptides on Titanium Surfaces

Recent strategies for improving hemocompatibility and endothelialization of cardiovascular devices and inhibition of intimal hyperplasia

Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

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