Covalent grafting of titanium with a cathelicidin peptide produces an osteoblast compatible surface with antistaphylococcal activity

Boix‐Lemonche, Gerard; Guillem-Marti, Jordi; D'Este, Francesca; Manero, José María; Skerlavaj, Barbara

doi:10.1016/j.colsurfb.2019.110586

Cited by 23 publications

(16 citation statements)

References 73 publications

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“…[ 44 , 45 ]). The modifications include, for example, the use of coatings with antibacterial properties due to the coating material itself [ 46 , 47 ], with polymers for local drug release [ 30 , 48 ], the “grafting” of the surface through the use of anchorage molecules [ 49 , 50 ], and the structuring of the surface with and without post coatings [ 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-loaded amphora-shaped pores on a titanium implant surface enhance osteointegration and prevent infections

Ständert

Borcherding

Bormann

et al. 2021

Bioactive Materials

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Artificial prostheses for joint replacement are indispensable in orthopedic surgery. Unfortunately, the implanted surface is attractive to not only host cells but also bacteria. To enable better osteointegration, a mechanically stable porous structure was created on a titanium surface using laser treatment and metallic silver particles were embedded in a hydrophilic titanium oxide layer on top. The laser structuring resulted in unique amphora-shaped pores. Due to their hydrophilic surface conditions and capillary forces, the pores can be loaded preoperative with the antibiotic of choice/need, such as gentamicin. Cytotoxicity and differentiation assays with primary human osteoblast-like cells revealed no negative effect of the surface modification with or without gentamicin loading. An in vivo biocompatibility study showed significantly enhanced osteointegration as measured by push-out testing and histomorphometry 56 days after the implantation of the K-wires into rat femora. Using a S. aureus infection model, the porous, silver-coated K-wires slightly reduced the signs of bone destruction, while the wires were still colonized after 28 days. Loading the amphora-shaped pores with gentamicin significantly reduced the histopathological signs of bone destruction and no bacteria were detected on the wires. Taken together, this novel surface modification can be applied to new or established orthopedic implants. It enables preoperative loading with the antibiotic of choice/need without further equipment or post-coating, and supports osteointegration without a negative effect of the released dug, such as gentamicin.

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Section: Discussionmentioning

confidence: 99%

Antibiotic-loaded amphora-shaped pores on a titanium implant surface enhance osteointegration and prevent infections

Ständert

Borcherding

Bormann

et al. 2021

Bioactive Materials

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“…LL-37 peptide-loaded nanopore structures onto Ti-based surface exhibits excellent bactericidal properties toward S. aureus and MRSA and bone-promoting capabilities in vitro and in non-infected and infected in vivo models [ 109 ]. α-helical cathelicidin-derived peptide BMAP27(1–18) grafted Ti disks considerably reduced S. epidermidis adhesion upon 2 h, and induced morphological alterations, exerting a rapid contact-killing effect [ 110 ]. Recently, Ti surfaces functionalized with a fusion peptide (FP), containing HHC36 antimicrobial and QK angiogenic peptides, exhibited over 96.8% in vitro antimicrobial activity against S. aureus , E. coli , P. aeruginosa and MRSA, while upregulating expressions of angiogenesis-related genes/proteins (VEGF and VEGFR-2) of human umbilical vein endothelial cell (HUVECs) and osteogenesis-related genes/proteins (ALP, COL-1, RUNX-2, OPN, and OCN) of hBMSCs [ 111 ].…”

Section: Promising Antimicrobial Compounds For the Treatment Of Cardiac And Orthopaedic Device-associated Infectionsmentioning

confidence: 99%

Trends in Managing Cardiac and Orthopaedic Device-Associated Infections by Using Therapeutic Biomaterials

et al. 2021

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Over the years, there has been an increasing number of cardiac and orthopaedic implanted medical devices, which has caused an increased incidence of device-associated infections. The surfaces of these indwelling devices are preferred sites for the development of biofilms that are potentially lethal for patients. Device-related infections form a large proportion of hospital-acquired infections and have a bearing on both morbidity and mortality. Treatment of these infections is limited to the use of systemic antibiotics with invasive revision surgeries, which had implications on healthcare burdens. The purpose of this review is to describe the main causes that lead to the onset of infection, highlighting both the biological and clinical pathophysiology. Both passive and active surface treatments have been used in the field of biomaterials to reduce the impact of these infections. This includes the use of antimicrobial peptides and ionic liquids in the preventive treatment of antibiotic-resistant biofilms. Thus far, multiple in vivo studies have shown efficacious effects against the antibiotic-resistant biofilm. However, this has yet to materialize in clinical medicine.

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“…It cannot be ignored that, in recent years, covalent grafting technology has gradually matured-usually by covalently linking some high-molecular-weight polymers and functionalized polymers to achieve antibacterial properties, such as polyNaSS polymers, hyaluronic acid, etc. [59]. Pichavant et al reported that they used covalent grafting technology to successfully connect vancomycin to titanium implants, and in vivo experiments It cannot be ignored that, in recent years, covalent grafting technology has gradually matured-usually by covalently linking some high-molecular-weight polymers and functionalized polymers to achieve antibacterial properties, such as polyNaSS polymers, hyaluronic acid, etc.…”

Section: Electrochemical Deposition and Vancomycinmentioning

confidence: 99%

“…Pichavant et al reported that they used covalent grafting technology to successfully connect vancomycin to titanium implants, and in vivo experiments It cannot be ignored that, in recent years, covalent grafting technology has gradually matured-usually by covalently linking some high-molecular-weight polymers and functionalized polymers to achieve antibacterial properties, such as polyNaSS polymers, hyaluronic acid, etc. [59]. Pichavant et al reported that they used covalent grafting technology to successfully connect vancomycin to titanium implants, and in vivo experiments confirmed its superior antibacterial properties [60].…”

Section: Electrochemical Deposition and Vancomycinmentioning

confidence: 99%

Titanium Implants and Local Drug Delivery Systems Become Mutual Promoters in Orthopedic Clinics

Gao

Zhao

et al. 2021

Nanomaterials

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Titanium implants have always been regarded as one of the gold standard treatments for orthopedic applications, but they still face challenges such as pain, bacterial infections, insufficient osseointegration, immune rejection, and difficulty in personalizing treatment in the clinic. These challenges may lead to the patients having to undergo a painful second operation, along with increased economic burden, but the use of drugs is actively solving these problems. The use of systemic drug delivery systems through oral, intravenous, and intramuscular injection of various drugs with different pharmacological properties has effectively reduced the levels of inflammation, lowered the risk of endophytic bacterial infection, and regulated the progress of bone tumor cells, processing and regulating the balance of bone metabolism around the titanium implants. However, due to the limitations of systemic drug delivery systems—such as pharmacokinetics, and the characteristics of bone tissue in the event of different forms of trauma or disease—sometimes the expected effect cannot be achieved. Meanwhile, titanium implants loaded with drugs for local administration have gradually attracted the attention of many researchers. This article reviews the latest developments in local drug delivery systems in recent years, detailing how various types of drugs cooperate with titanium implants to enhance antibacterial, antitumor, and osseointegration effects. Additionally, we summarize the improved technology of titanium implants for drug loading and the control of drug release, along with molecular mechanisms of bone regeneration and vascularization. Finally, we lay out some future prospects in this field.

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Covalent grafting of titanium with a cathelicidin peptide produces an osteoblast compatible surface with antistaphylococcal activity

Cited by 23 publications

References 73 publications

Antibiotic-loaded amphora-shaped pores on a titanium implant surface enhance osteointegration and prevent infections

Antibiotic-loaded amphora-shaped pores on a titanium implant surface enhance osteointegration and prevent infections

Trends in Managing Cardiac and Orthopaedic Device-Associated Infections by Using Therapeutic Biomaterials

Titanium Implants and Local Drug Delivery Systems Become Mutual Promoters in Orthopedic Clinics

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