Covalently Assembled Bifunctional Copolymer Layers as a Matrix for Immobilization of Oligonucleotides

Kim, Ji Soo; Yoo, Sangwook; Kim, Jung-Ahn; Kim, Joo-Hoon

doi:10.5012/bkcs.2012.33.4.1401

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…In an effort to extend the detection technology to more cost-effective and portable devices, the development of various functional surfaces immobilized with probe nucleic acids has been reported. − In one example, Zhang et al immobilized DNA capture probes on paper substrates to detect viral RNAs . Polymer-grafted surfaces have also been utilized for nucleic acid immobilization. − Aied et al reported surface-initiated atom transfer radical polymerization (ATRP) to prepare cationic polymer brushes . Negatively charged probe DNAs were then captured on the polymer brushes and used in combination with fluorescent molecules to detect DNA target.…”

Section: Introductionmentioning

confidence: 99%

“…Other reports included the use of functional polymers to immobilize nucleic acids. For example, Kim et al prepared a random copolymer containing bioinert poly(ethylene glycol) methyl ether methacrylate and bioactive p -formylphenyl methacrylate for the immobilization of DNAs on glass substrates . Thomson et al prepared azide-functionalized polymer brushes and used CuAAC click reaction to conjugate alkyne-terminated DNA probes .…”

Section: Introductionmentioning

confidence: 99%

“…For example, Kim et al prepared a random copolymer containing bioinert poly(ethylene glycol) methyl ether methacrylate and bioactive p-formylphenyl methacrylate for the immobilization of DNAs on glass substrates. 37 Thomson et al prepared azidefunctionalized polymer brushes and used CuAAC click reaction to conjugate alkyne-terminated DNA probes. 36 In combination with the use of magnetic particles, detection of DNA target with improved sensitivity was reported.…”

Section: ■ Introductionmentioning

confidence: 99%

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Reactive Polymer Targeting dsRNA as Universal Virus Detection Platform with Enhanced Sensitivity

Kim

Park

et al. 2020

Biomacromolecules

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Reactive poly(pentafluorophenyl acrylate) (PPFPA)-grafted surfaces offer a versatile platform to immobilize biomolecules. Here, we utilize PPFPA-grafted surface and double-stranded RNA (dsRNA) recognizing J2 antibody to construct a universal virus detection platform with enhanced sensitivity. PPFPA on silicon substrates is prepared, and surface hydrophilicity is modulated by partial substitution of the pentafluorophenyl units with poly(ethylene glycol). Following dsRNA antibody immobilization, the prepared surfaces can distinguish long dsRNAs from single-stranded RNAs of the same length and short dsRNAs. As long dsRNAs are common byproducts of viral transcription/replication, these surfaces can detect the presence of different kinds of viruses without prior knowledge of their genomic sequences. To increase dsRNA detection sensitivity, a two-step method is devised where the captured dsRNAs are visualized with multiple fluorophore-tagged J2 antibodies. We show that the developed platform can differentiate foreign long dsRNAs from cellular dsRNAs and other biomolecules present in the cell lysate. Moreover, when tested against cells infected with hepatitis A or C viruses, both viruses are successfully detected using a single platform. Our study shows that the developed PPFPA platform immobilized with J2 antibody can serve as a primary diagnostic tool to determine the infection status for a wide range of viruses.

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