COVID-19 Diagnostics: Past, Present, and Future

Scholtz, Alexis; Ramoji, Anuradha; Silge, Anja; Jansson, Jakob R.; Moura, Ian G. de; Sram, Jakub P.; Armani, Andrea M.

doi:10.1021/acsphotonics.1c01052

Cited by 15 publications

(14 citation statements)

References 77 publications

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“…Cell-based functional microarrays are often used to gain insight into virus-host interactions by in vitro immune cell-viral interactions studies [135]. For example, the PRR of immune cells for targeted sensing of microbes is well studied using different fluorescence imaging techniques [132].…”

Section: Immune Response Toward Virusmentioning

confidence: 99%

“…However, information on the host's immune status during infection is obtained by comparing the changes in the biochemical profile of the healthy and the disease state [155,156]. An intriguing option for identifying viral infections is to apply vibrational spectroscopic techniques combined with multivariate analysis [157] because this enables the investigation of highly complex samples without the need for extensive sample preparation [135,158]. A viral infection will result in characteristic chemical changes of the affected tissue or body fluid [118].…”

Section: Study Of Host's Immune Status During Viral Infectionmentioning

confidence: 99%

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Understanding viruses and viral infections by biophotonic methods

Ramoji

Pahlow

Pistiki

et al. 2022

Transl Biophotonics

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In the last few decades outbreaks of viral infections have often challenged the world‐wide health infrastructure and caused a significant financial burden as well as human suffering despite progress in diagnostic technologies. The recent outbreaks of the Ebola virus in the African continent, the Zika virus in the American continent, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), influenza A and lately severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) viral infections have repeatedly highlighted the importance of technological advancement enabling a better understanding of virions. In this review, we systematically discuss different aspects of virions and how their properties and functions can be studied using different light‐based technologies. We focus on virion classification, detection and interactions with the host's immune system. Further, the potential of advanced biophotonic methods, for example, Raman, infrared reflection, absorption and fluorescence spectroscopy, advanced microscopic techniques and biosensor‐based approaches for diagnosing viral infections, investigating therapeutics and vaccine development are described. Although significant advancements have already been made in photonic technologies, which even enable visualizing virion‐host interactions on single‐cell level, the continuous evolution of viruses demands further progress in biophotonic solutions for fast, affordable and robust health monitoring devices for screening viral infections.

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Section: Immune Response Toward Virusmentioning

confidence: 99%

Section: Study Of Host's Immune Status During Viral Infectionmentioning

confidence: 99%

Understanding viruses and viral infections by biophotonic methods

Ramoji

Pahlow

Pistiki

et al. 2022

Transl Biophotonics

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“…Different viral dilutions to mimic saliva conditions were been used to detect RNA virus using Raman spectroscopy 26 . Recent studies reported the detection of SARS-CoV-2 using surface-enhanced Raman scattering (SERS) 1 , 27 – 32 . SERS results coupled with PCA analysis is been introduced as a powerful tool to detect the virus 33 .…”

Section: Introductionmentioning

confidence: 99%

Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy

et al. 2022

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SARS-CoV-2 infection remains spread worldwide and requires a better understanding of virus-host interactions. Here, we analyzed biochemical modifications due to SARS-CoV-2 infection in cells by confocal Raman microscopy. Obtained results were compared with the infection with another RNA virus, the measles virus. Our results have demonstrated a virus-specific Raman molecular signature, reflecting intracellular modification during each infection. Advanced data analysis has been used to distinguish non-infected versus infected cells for two RNA viruses. Further, classification between non-infected and SARS-CoV-2 and measles virus-infected cells yielded an accuracy of 98.9 and 97.2 respectively, with a significant increase of the essential amino-acid tryptophan in SARS-CoV-2-infected cells. These results present proof of concept for the application of Raman spectroscopy to study virus-host interaction and to identify factors that contribute to the efficient SARS-CoV-2 infection and may thus provide novel insights on viral pathogenesis, targets of therapeutic intervention and development of new COVID-19 biomarkers.

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“…Viral infection with different incubations time has been monitored in previous studies 26,27 . Recent studies reported the detection of SARS-CoV-2 using Surface Enhanced Raman Scattering (SERS) 2,[28][29][30][31][32][33] . SERS substrate design dictates sensitivity and accuracy of the method and presents an essential key towards a successful result, but is also associated to a rather high cost of analysis [30][31][32]34 .…”

Section: Introductionmentioning

confidence: 99%

Specific Intracellular Signature of SARS-CoV-2 Infection Using Confocal Raman Microscopy

Salehi

Ramoji

Mougari

et al. 2022

Preprint

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SARS-CoV-2 infection remains to spread worldwide and requires a better understanding of virus-host interactions. Here, we analyzed biochemical modifications due to SARS-CoV-2 infection in cells by confocal Raman microscopy. Obtained results were compared with the infection with another RNA virus, the measles virus. Our results have demonstrated a virus-specific Raman hallmark of molecular signature, reflecting intracellular modification during each infection. Advanced data analysis has been used to distinguish non-infected versus infected cells for two RNA viruses. Further, classification between non-infected and SARS-CoV-2 and measles virus-infected cells yielded an accuracy of 98.9 and 97.2 respectively, with a significant increase of the essential amino-acid tryptophan in SARS-CoV-2-infected cells. These results present proof of concept for the application of Raman spectroscopy to study virus-host interaction and to identify factors that contribute to the efficient SARS-CoV-2 infection and may thus provide novel insights on viral pathogenesis, targets of therapeutic intervention and development of new COVID-19 biomarkers.

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COVID-19 Diagnostics: Past, Present, and Future

Cited by 15 publications

References 77 publications

Understanding viruses and viral infections by biophotonic methods

Understanding viruses and viral infections by biophotonic methods

Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy

Specific Intracellular Signature of SARS-CoV-2 Infection Using Confocal Raman Microscopy

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