Coxiella burnetii associated placental lesions and infection level in parturient cows

Hansen, Mette Sif; Rodolakis, Annie; Cochonneau, Denis; Agger, Jens Frederik; Christoffersen, Anna-Bodil; Jensen, Tim Kåre; Agerholm, Jørgen Steen

doi:10.1016/j.tvjl.2010.12.021

Cited by 53 publications

(72 citation statements)

References 17 publications

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“…FISH has been used in veterinary reports to detect C. burnetii in ruminant placentas using both IS1111 and 16S rRNA probes (40,41). Its use to detect C. burnetii in human lymph nodes has only recently been published (34).…”

Section: Discussionmentioning

confidence: 99%

Mouse Model of Coxiella burnetii Aerosolization

et al. 2016

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cCoxiella burnetii is mainly transmitted by aerosols and is responsible for multiple-organ lesions. Animal models have shown C. burnetii pathogenicity, but long-term outcomes still need to be clarified. We used a whole-body aerosol inhalation exposure system to mimic the natural route of infection in immunocompetent (BALB/c) and severe combined immunodeficient (SCID) mice. After an initial lung inoculum of 10 4 C. burnetii cells/lung, the outcome, serological response, hematological disorders, and deep organ lesions were described up to 3 months postinfection. C. burnetii-specific PCR, anti-C. burnetii immunohistochemistry, and fluorescent in situ hybridization (FISH) targeting C. burnetii-specific 16S rRNA completed the detection of the bacterium in the tissues. In BALB/c mice, a thrombocytopenia and lymphopenia were first observed, prior to evidence of C. burnetii replication. In all SCID mouse organs, DNA copies increased to higher levels over time than in BALB/c ones. Clinical signs of discomfort appeared in SCID mice, so follow-up had to be shortened to 2 months in this group. At this stage, all animals presented bone, cervical, and heart lesions. The presence of C. burnetii could be attested in situ for all organs sampled using immunohistochemistry and FISH. This mouse model described C. burnetii Nine Mile strain spread using aerosolization in a way that corroborates the pathogenicity of Q fever described in humans and completes previously published data in mouse models. C. burnetii infection occurring after aerosolization in mice thus seems to be a useful tool to compare the pathogenicity of different strains of C. burnetii.

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Section: Discussionmentioning

confidence: 99%

Mouse Model of Coxiella burnetii Aerosolization

et al. 2016

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“…Detection of bacterial shedders is important to investigate the spread of C. burnetii among animals and from animals to humans and to assess the potential zoonotic risk. Coxiella burnetii is shed mainly in birth products such as fluids and placenta, but may also be excreted in feces, urine, vaginal mucus, and milk (Rodolakis, 2009;Hansen et al, 2011). In cattle, milk has been shown to be a significant route of shedding (Guatteo et al, 2007a) and indications exist that C. burnetii may induce inflammation of the mammary gland in cattle (Barlow et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle

Angen

Ståhl

Agerholm

et al. 2011

Journal of Dairy Science

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“…The prevalence of infection was 35%, which falls within the range reported in the international literature ( The sensitivity and specificity of the ELISA assay used in the present study are unknown and, hence, the true prevalence of the disease may be different from what is reported herein. Nonetheless, most research groups utilize either ELISA or complement fixation test for bulk milk antibody detection, which appear to be similar in terms of sensitivity and specificity (Hansen et al 2011, Schalch et al 1998). Seropositive animals were found in herds that were characterized as negative on the basis of antibody presence in the bulk milk.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Coxiella burnetii antibodies in bulk milk and blood serum and associations with reproductive indices in cow dairy herds of Central and Northern Greece

Dovolou

Tsiligianni

Βουζαρασ)

et al. 2017

J Hellenic Vet Med Soc

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For the first time in Greece, we investigated the prevalence of Coxiella burnetii antibodies in milk and sera from dairy cattle herds located at central and northern parts of the country. Eighty herds were initially voluntary enrolled in the study and a bulk milk sample from each farm was assayed by ELISA for C burnetii antibodies. According to antibody titre, herds were classified into 5 categories: negative and grades 1,2,3 and 4 (ascending scale). To assess the prevalence within farms, two herds from each category were selected and blood samples were collected for antibody assessment. In these herds, some reproductive indices were compared between farms; in addition, comparisons were made in paired seropositive and seronegative animals from one grade 3 herd. Twenty three herds (35%) were found positive, 21 being in categories 3 and 4. The prevalence of seropositive animals between herds varied from 4.9 to 46.3%, even from farms initially characterized as negative, some positive animals were detected. Between farms, no differences were detected in the abortion rate or in the mean number of artificial inseminations (AI) per pregnancy. Some differences were found in other reproductive indices that were impossible to be biologically interpreted under the light of C. burnetii level of infection. From the results presented here, we infer that C. burnetii infection is likely asymptomatic in dairy cows causing minimal -if any- economic losses to farmers. However, since the disease is a zoonotic one, its spread can easily occur, a systematic surveillance, in all ruminant species, for the restriction or eradication of the disease should be undertaken in national level.

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Coxiella burnetii associated placental lesions and infection level in parturient cows

Cited by 53 publications

References 17 publications

Mouse Model of Coxiella burnetii Aerosolization

Mouse Model of Coxiella burnetii Aerosolization

Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle

Prevalence of Coxiella burnetii antibodies in bulk milk and blood serum and associations with reproductive indices in cow dairy herds of Central and Northern Greece

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