2003
DOI: 10.1074/jbc.m212473200
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CpG Oligodeoxynucleotides Modulate the Osteoclastogenic Activity of Osteoblasts via Toll-like Receptor 9

Abstract: Regulation of osteoclastogenesis by lipopolysaccharide (LPS) is mediated via its interactions with toll-like

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Cited by 91 publications
(91 citation statements)
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“…Consistently higher levels of TNF-␣ were released from BALB/c-derived cells in response to CpG-ODN challenge. We have shown that in cultures containing both osteoclast and osteoblast-lineage cells CpG-ODN induces TNF-␣ in both cell types (9). It was therefore important for the present study that the cultures did not contain osteoblastic or mesenchymal cells.…”
Section: Discussionmentioning
confidence: 99%
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“…Consistently higher levels of TNF-␣ were released from BALB/c-derived cells in response to CpG-ODN challenge. We have shown that in cultures containing both osteoclast and osteoblast-lineage cells CpG-ODN induces TNF-␣ in both cell types (9). It was therefore important for the present study that the cultures did not contain osteoblastic or mesenchymal cells.…”
Section: Discussionmentioning
confidence: 99%
“…Bacterial factors have been studied extensively for their ability to activate the innate immune system via Toll-like receptors (TLRs) 1 present on the host immune cells (6,7). Bone cells also express TLRs enabling the bacteria-derived factors to exert their effects on the skeleton (8,9). The most studied bacterial factor in this regard is lipopolysaccharide, recognized by both osteoclast and osteoblast lineage cells via TLR4 (8,9).…”
mentioning
confidence: 99%
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“…Cell activation Neutrophils (5610 6 cells?mL -1 ) were incubated for 20-30 min with L-NAME (0.1-10 mM) or NAC (0.1-10 mM); chloroquine (1-50 mg?mL -1 ) (enhances endosomal pH and thereby blocks lysomal pathways and used here as a blocker of TLRs signalling) [26] or blocking anti-sense ODN (1-10 mM) and then stimulated with CSE (1.5%), CpG ODN type A (3 mM) or control ODN (5 mM) for 30 min for western blot analysis. For CXCL8 determination 3610 6 cells?mL -1 were incubated for 9 h according to the conditions for Western blot analysis.…”
Section: Isolation Of Neutrophilsmentioning
confidence: 99%
“…Similar to LPS, CpG DNA modulates osteoclastogenesis in bone marrow cell/osteoblast cocultures. Upon TLR-9 ligation, CpG DNA interacts with osteoblastic TLR-9 and elicits intracellular events leading to the increased expression of molecules regulating osteoclastogenesis (59).…”
Section: Bacterial Dnamentioning
confidence: 99%