Cranberry phytochemical extracts induce cell cycle arrest and apoptosis in human MCF-7 breast cancer cells

Sun, Jie; Liu, Rui Hai

doi:10.1016/j.canlet.2005.10.027

Cited by 142 publications

(124 citation statements)

References 48 publications

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“…The fluorescence intensity of sub G 0 cell fraction represented the apoptotic cell population. Previous studies revealed that anticancer agents arrest the cell cycle at the G 0 / G 1 , S or G 2 /M phase and then induce apoptotic cell death [15,24,29] . The cell cycle arrest has become an appreciated target for management and treatment of tumor cells with cytotoxic agents.…”

Section: Resultsmentioning

confidence: 99%

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

Chaudhary¹,

Choudhary²,

Sharma³

et al. 2016

IJPS

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Chaudhary, et al.: In vitro Evaluation of Brassica SproutsThe present study was conducted on sprouts (5 and 7 days) of turnip (Brassica rapa), cauliflower (Brassica oleracea) and mustard (Brassica juncea) for bioactivity. Their antioxidant potential was assessed by 2,2-diphenyl-1-picrylhydrazyl, superoxide anion radical scavenging assays at 0.125-2 mg/ml concentration. The extract of turnip, cauliflower and mustard (5 and 7 days) showed a potent antioxidant effect and significant cytotoxic effect at 100 µg/ml concentration of extract. The antiproliferative potential was also evaluated by applying cell cycle and intracellular reactive oxygen species generation assay at IC 50 value. Various phytochemicals and hydrolytic products of glucosinolates were observed in a different extract of turnip, cauliflower, and mustard sprouts. Flow cytometric analysis showed that all the extracts caused an increase in a G 0 population of the PC-3 cells in cell cycle analysis and an increase in intracellular reactive oxygen species generation as compared to untreated cells. Confocal imaging of the cells stained with 4',6-diamidino-2-phenylindole and 2,7-dichlorodihydrofluorescein diacetate showed DNA fragmentation and increase of fluorescence which supports apoptosis and intracellular generation of the reactive oxygen species as the possible cause of cell death. The reported activity was correlated with the presence of different organosulfur compounds identified by gas chromatography-mass spectroscopy. Key words: Brassica sprout, antioxidant assay, cell cycle, ROS, confocal microscopy, MTT assayIn recent years, health protection by natural products or plant derived foods has received a considerable attention [1] . Specific groups of vegetables are particularly rich in potentially protective phytochemicals, especially Brassica contains a high concentration of constituents with antioxidant properties (e.g. carotenoids, vitamin C and folates) as well as glucosinolates precursors of isothiocyanates (ITCs) and indoles that modulate the activity of xenobiotic biotransformation [2] . A diet rich in fruits, cruciferous vegetables has been linked to reduce the risk of many chronic diseases, including cancer. The importance of this family for food crops has led to its selective breeding throughout the history. Cruciferous sprouts of broccoli, alfalfa, buckwheat and bean etc. have received considerable attention due to their rich content of health-promoting phytochemical constituents such as glucosinolates phenolic compounds and ascorbic acid related to cancer prevention as well as having antioxidant properties [3][4][5][6][7] .During sprouting generally, phytonutrient content increases as compared to seeds and consumption of these sprouts is the best way to gain all the health benefits [8,9] . Naturally occurring ITCs, found abundantly in cruciferous vegetables, inhibit tumorigenesis by inducing apoptosis and arresting cell cycle progression. In addition, induction of detoxification enzymes suggested the mechanism through which cruciferous v...

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Section: Resultsmentioning

confidence: 99%

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

Chaudhary¹,

Choudhary²,

Sharma³

et al. 2016

IJPS

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“…Chemoprevention has been successfully achieved in numerous in vitro and as well as in vivo studies and has been validated in several human intervention trials (Lee et al, 2005). Chemopreventive agents have low side effects and toxicity and are involved in neutralization of carcinogenic agents as well as their effects on cells (Sun et al, 2006). In recent years, increasing attention has been focused on to identify the naturally occurring chemopreventive agents, particularly those present in dietary and medicinal plants due to their bioactive substances (Sebastian et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Fenugreek Induced Apoptosis in Breast Cancer MCF-7 Cells Mediated Independently by Fas Receptor Change

Alshatwi

Shafi²,

Hasan³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Trigonella foenum in graecum (Fenugreek) is a traditional herbal plant used to treat disorders like diabetes, high cholesterol, wounds, inflammation, gastrointestinal ailments, and it is believed to have anti-tumor properties, although the mechanisms for the activity remain to be elucidated. In this study, we prepared a methanol extract from Fenugreek whole plants and investigated the mechanism involved in its growth-inhibitory effect on MCF-7 human breast cancer cells. Apoptosis of MCF-7 cells was evidenced by investigating trypan blue exclusion, TUNEL and Caspase 3, 8, 9, p53, FADD, Bax and Bak by real-time PCR assays inducing activities, in the presence of FME at 65 µg/mL for 24 and 48 hours. FME induced apoptosis was mediated by the death receptor pathway as demonstrated by the increased level of Fas receptor expression after FME treatment. However, such change was found to be absent in Caspase 3, 8, 9, p53, FADD, Bax and Bak, which was confirmed by a time-dependent and dose-dependent manner. In summary, these data demonstrate that at least 90% of FME induced apoptosis in breast cell is mediated by Fas receptor-independently of either FADD, Caspase 8 or 3, as well as p53 interdependently.

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“…An organic soluble cranberry extract induces apoptosis in stomach (5.0 mg/mL) and breast (15.0 mg/mL) cancer cell lines at high concentrations, whereas cancer cell death was induced at much lower concentrations (range of 50.0-70.0 µg/mL) in SCC25 and CAL27 oral cancer cell lines [23,46,53]. Treatment of esophageal adenocarcinoma, lung, colon, glioblastoma, neuroblastoma and ovarian cancer cells with cranberry proanthocyanidins results in apoptosis induction [35][36][37]39,42,44,48,49].…”

Section: Cranberry Derived Extracts and Constituents Induce Cell Deatmentioning

confidence: 99%

Cranberries and Cancer: An Update of Preclinical Studies Evaluating the Cancer Inhibitory Potential of Cranberry and Cranberry Derived Constituents

Weh

Clarke

Kresty

2016

Preprint

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Abstract:Cranberries are rich in bioactive constituents reported to influence a variety of health benefits, ranging from improved immune function and decreased infections to reduced cardiovascular disease and more recently cancer inhibition. A review of cranberry research targeting cancer revealed positive effects of cranberries or cranberry derived constituents against 17 different cancers utilizing a variety of in vitro techniques, whereas in vivo studies supported the inhibitory action of cranberries toward cancers of the esophagus, stomach, colon, bladder, prostate, glioblastoma and lymphoma. Mechanisms of cranberry-linked cancer inhibition include cellular death induction via apoptosis, necrosis and autophagy; reduction of cellular proliferation; alterations in reactive oxygen species; and modification of cytokine and signal transduction pathways. Given the emerging positive preclinical effects of cranberries, future clinical directions targeting cancer or premalignancy in high risk cohorts should be considered.

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Cranberry phytochemical extracts induce cell cycle arrest and apoptosis in human MCF-7 breast cancer cells

Cited by 142 publications

References 48 publications

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential

Fenugreek Induced Apoptosis in Breast Cancer MCF-7 Cells Mediated Independently by Fas Receptor Change

Cranberries and Cancer: An Update of Preclinical Studies Evaluating the Cancer Inhibitory Potential of Cranberry and Cranberry Derived Constituents

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